Abstract Accurate geospatial information about the causes and consequences of climate change, including energy systems infrastructure, is critical to planning climate change mitigation and adaptation strategies. When up-to-date spatial data on infrastructure is lacking, one approach to fill this gap is to learn from overhead imagery using deep-learning-based object detection algorithms. However, the performance of these algorithms can suffer when applied to diverse geographies, which is a common case. We propose a technique to generate realistic synthetic overhead images of an object (e.g., a generator) to enhance the ability of these techniques to transfer across diverse geographic domains. Our technique blends example objects into unlabeled images from the target domain using generative adversarial networks. This requires minimal labeled examples of the target object and is computationally efficient such that it can be used to generate a large corpus of synthetic imagery. We show that including these synthetic images in the training of an object detection model improves its ability to generalize to new domains (measured in terms of average precision) when compared to a baseline model and other relevant domain adaptation techniques.
Despite the success of antiretroviral therapy, human immunodeficiency virus (HIV) cannot be cured because of a reservoir of latently infected cells that evades therapy. To understand the mechanisms of HIV latency, we employed an integrated single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin with sequencing (scATAC-seq) approach to simultaneously profile the transcriptomic and epigenomic characteristics of ∼ 125,000 latently infected primary CD4+ T cells after reactivation using three different latency reversing agents. Differentially expressed genes and differentially accessible motifs were used to examine transcriptional pathways and transcription factor (TF) activities across the cell population. We identified cellular transcripts and TFs whose expression/activity was correlated with viral reactivation and demonstrated that a machine learning model trained on these data was 75%-79% accurate at predicting viral reactivation. Finally, we validated the role of two candidate HIV-regulating factors, FOXP1 and GATA3, in viral transcription. These data demonstrate the power of integrated multimodal single-cell analysis to uncover novel relationships between host cell factors and HIV latency.
