M. Porkodi

Central Institute of Fisheries Education

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Manoj P. Brahmane

Central Institute of Fisheries Education

N. S. Nagpure

Central Institute of Fisheries Education

Kiran D. Rasal

Central Institute of Fisheries Education

Balakrishnan Vaitheeswari

Ganapathy Sindhu

Annamalai University

Gowhar Iqbal

Central Institute of Fisheries Education

Nalini Poojary

Central Institute of Fisheries Education

Pachaiappan Pugalendi

Annamalai University

Arvind Sonwane

Central Institute of Fisheries Education

M Harshavarthini

Central Institute of Fisheries Education

Cooperative Institutions

Central Institute of Fisheries Education

Indian Council of Agricultural Research

National Institute of Abiotic Stress Management

Annamalai University

Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir

Central Institute of Freshwater Aquaculture

Nizam's Institute of Medical Sciences

National Institute for Interdisciplinary Science and Technology

University of Kerala

Central Inland Fisheries Research Institute

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Indigo dyes: Toxicity, teratogenicity, and genotoxicity studies in zebrafish embryos

Mutation Research/Genetic Toxicology and Environmental Mutagenesis (2024)

M. Porkodi Manoj P. Brahmane Mujahidkhan A. Pathan Nalini Poojary Shubra Singh

Comet Assay

10.1016/j.mrgentox.2024.503752

Cite

Citations (2)

Identification of Gin-α Hyperactivated Recombinase-based RNA-Guided Recombinase Platform Target Sites in the Zebrafish Genome

Indian Journal of Animal Research (2025)

Nahida Quyoom M. Porkodi Gowhar Iqbal Kiran D. Rasal Manoj P. Brahmane

Background: RNA-guided recombinase (RGR) platform is recombinase action-based genome engineering platform. It is a fusion of dead Cas9 (dCas9) and evolved recombinase with relaxed target specificity. RGR is facile, programmable and has high genome targeting capacity and a safer alternative to conventional nuclease-based methods, which carry a risk of genotoxicity. The present study was conducted to map the entire zebrafish genome for the locations of Gin-α hyperactivated recombinase-based RGR platform target sites with varying spacer lengths. Methods: RGR platform target site DNA patterns with varying spacer lengths (5/6/7-base pair) were searched in all zebrafish chromosomes using the NCBI Genome Data Viewer (GDV) genome browser. Genomic contexts of the located RGR platform target sites were further documented using NCBI Blast. Result: The study identified 262 RGR platform target sites with 5-bp spacer, 307 sites with 6-bp spacer and 326 sites with 7-bp spacer. It also analyzed the genomic context of these sites, identifying zebrafish genes located near the identified target sites. This work contributes to the development of genome editing techniques that are highly specific and safer, as well as programmable and simple and provides tools to utilize them in model organisms like zebrafish, which are commonly used in various genetic and biomedical studies.

Identification

10.18805/ijar.b-5495

Cite

Citations (0)

Trianthema decandra Root Extract and Cisplatin Action on Biochemical and Histological Characterization of Oral Cancer in DMBA-induced Preclinical Hamster Model

Asian Journal of Biological and Life Sciences (2023)

M. Porkodi Ganapathy Sindhu Kumarasamypillai Radha Thayammal Asha Veerasamy Vinothkumar Pachaiappan Pugalendi

Aim: Chemoprevention, an emerging promising technique for experimental oncology, focuses on the use of natural creates to inhibit or prevent the progression of malignancy. In this study, the chemopreventive ability of TD (Trianthema decandra) root extract was studied in regard to DMBAinduced HBPs. Materials and Methods: Painted the left Buccal pouches mixture of 0.5% DMBA in liquid paraffin weekly thrice for 14 weeks, and the result was the development of buccal cancer. In hamsters given in two ways, DMBA alone or DMBA+TD root extract treatment, tumour development measurement, detoxification agents, Lipid Peroxidation (LPO), antioxidant status, renal function markers and histological alterations were evaluated. Results: Hamsters provided the treatment demonstrated 100% tumour happenings with a DMBA alone causes an imbalance in the Enzymes to break up carcinogens renal function identifiers and cellular redox state. When administered orally to hamsters undergoing DMBA medication, TD root extract, entirely prevented the formation of tumors and Cellular redox state and enzymes that break down carcinogens. Conclusion: TD root extract will be a conventional medicine for rejuvenating and nephrogenic remedies, and shows promising chemopreventive efficacy in DMBA-induced HBPs Cancer.

10.5530/ajbls.2023.12.35

Cite

Citations (0)