Roberta Palumbo

Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana

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Marco E. Bianchi

Vita-Salute San Raffaele University

Maurizio C. Capogrossi

Johns Hopkins Medicine

Eric A. Rose

Christ University

John Dent

University of Adelaide

Dennis Buxton

Jefferson Baer

Emory University

Deborah D. Ascheim

Capricor Therapeutics (United States)

Michael Siegenthaler

Swiss Finance Institute

Roger Swayze

Albert Einstein College of Medicine

Ann Greenberg

Caesars Entertainment (United States)

Cooperative Institutions

Icahn School of Medicine at Mount Sinai

Istituti di Ricovero e Cura a Carattere Scientifico

Istituto Dermopatico dell'Immacolata

National Institutes of Health

Harvard University

Massachusetts General Hospital

Cleveland Clinic

Duke University

University of Toronto

Brigham and Women's Hospital

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High mobility group B2 is secreted by myeloid cells and has mitogenic and chemoattractant activities similar to high mobility group B1

Autoimmunity (2009)

Tobias Pusterla Francesco De Marchis Roberta Palumbo Marco E. Bianchi

High mobility group B box (HMGB) proteins are a family of chromatin proteins made up of two basic DNA binding domains, HMG box A and B, and a C-terminal acidic tail. HMGB have a highly conserved sequence, but different expression pattern: HMGB1 is almost ubiquitous, whereas the others are highly expressed in only a few tissues in adults. We previously demonstrated that HMGB1 is released by necrotic cells and has chemoattractant activity for inflammatory and stem cells, via binding to receptor for advanced glycation endproducts (RAGE). HMGB1 can be actively secreted by inflammatory cells. Here, we report that also HMGB2 can be secreted by THP-1 cells, and promotes proliferation and migration of endothelial cells. These functions of HMGB2 are exerted via engagement of RAGE, whose blockade completely abrogates cell responses. Since extracellular HMGB2 has been detected in the blood and other biological fluids, it might be necessary to target HMGB2 at the same time as HMGB1 for therapeutical efficacy.

HMGB1

High-mobility group

RAGE

10.1080/08916930902831845

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Citations (49)

Exercise Training and Cardiac Rehabilitation in COVID-19 Patients with Cardiovascular Complications: State of Art

Life (2021)

Mariaconsiglia Calabrese Marina Garofano Roberta Palumbo Paola Di Pietro Carmine Izzo

Recent scientific literature has investigated the cardiovascular implications of COVID-19. The mechanisms of cardiovascular damage seem to involve the protein angiotensin-converting enzyme 2 (ACE2), to which severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) binds to penetrate cells and other mechanisms, most of which are still under study. Cardiovascular sequelae of COVID-19 include heart failure, cardiomyopathy, acute coronary syndrome, arrhythmias, and venous thromboembolism. This article aims to collect scientific evidence by exploiting PubMed, Scopus, and Pedro databases to highlight the cardiovascular complications of COVID-19 and to define the physiotherapy treatment recommended for these patients. Exercise training (ET), an important part of cardiac rehabilitation, is a powerful tool in physiotherapy, capable of inducing significant changes in the cardiovascular system and functional in the recovery of endothelial dysfunction and for the containment of thromboembolic complications. In conclusion, due to the wide variety of possible exercise programs that can be obtained by combining intensity, duration, and speed in various ways, and by adjusting the program based on continuous patient monitoring, exercise training is well suited to the treatment of post-COVID patients with an impaired cardiovascular system of various degrees.

Aerobic Exercise

10.3390/life11030259

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Citations (68)

Rehabilitation need for migrant populations: physiotherapy treatment of the PostTraumatic Stress Disorder and chronic pain from torture results

Journal of Advanced Health Care (2020)

Marina Garofano Matteo Maria Ascoli Roberta Palumbo

The migration phenomenon in recent years is assuming considerable proportions, so it is necessary to consider theneed for health of migrant populations. The present work investigate, in particular, the rehabilitation need of themigrant populations. From the analysis of the literature about the incidence and prevalence of the deseases, two conditions of physiotherapic interest come to light: Post Traumatic Stress Disorder (PTSD) and the rehabilitation fromtorture outcomes. The analysis of the literature was carried out on the most important databases: PubMed, Scopusand PEDro.In PTSD relaxation techniques like BBAT appear to be essential In chronic pain from torture, manual therapy, progressive exercises, balance training are recommended. Unfortunately, the rehabilitative intervention in no study iswell clarified in terms of duration, frequency and type of exercise, so repeatability is definitely invalidated and theeffectiveness of it continues to be uncertain.

Torture

10.36017/jahc20202149

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Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-κB activation

The Journal of Cell Biology (2007)

Roberta Palumbo Beatriz G. Gálvez Tobias Pusterla Francesco De Marchis Giulio Cossu

Tissue damage is usually followed by healing, as both differentiated and stem cells migrate to replace dead or damaged cells. Mesoangioblasts (vessel-associated stem cells that can repair muscles) and fibroblasts migrate toward soluble factors released by damaged tissue. Two such factors are high mobility group box 1 (HMGB1), a nuclear protein that is released by cells undergoing unscheduled death (necrosis) but not by apoptotic cells, and stromal derived factor (SDF)–1/CXCL12. We find that HMGB1 activates the canonical nuclear factor κB (NF-κB) pathway via extracellular signal-regulated kinase phosphorylation. NF-κB signaling is necessary for chemotaxis toward HMGB1 and SDF-1/CXCL12, but not toward growth factor platelet-derived growth factor, formyl-met-leu-phe (a peptide that mimics bacterial invasion), or the archetypal NF-κB–activating signal tumor necrosis factor α. In dystrophic mice, mesoangioblasts injected into the general circulation ingress inefficiently into muscles if their NF-κB signaling pathway is disabled. These findings suggest that NF-κB signaling controls tissue regeneration in addition to early events in inflammation.

HMGB1

10.1083/jcb.200704015

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Citations (247)

Experimental tutoring project: from guidelines to clinical practice

Journal of Advanced Health Care (2020)

Mariaconsiglia Calabrese Roberta Palumbo Marina Garofano Maria Chiara Lauro Eros De Simone

Promote interdisciplinary tutoring activities in order to teach students how to work in team developing trans-skill competences. Actively involve patients in the construction and management of the assistential and rehabilitative activities to improve adherence and compliance. At Salerno University an experimental pedagogy project involved third-year students attending the Degree Course in Physiotherapy, Nursing, and the fifth year of Medicine, who performed a professionalized clinical internship at the Complex Operative Unit (UOC) of Cardiac Rehabilitation (CR), University Hospital “San Giovanni di Dio Ruggi d’Aragona” in Salerno. We divided students (60) and patients (100) in two groups, experimental and control one, each of them composed by 30 students and 50 cardio-operated patients. Patients are entrusted to an interdisciplinary team of tutors and students, guided by a team-leader chosen in rotation between students. After defining the patient’s need for care and rehabilitation, customized programs were designed, encouraging an active participation of the patient. We gave a self-assessment questionnaire to students for learned skill assessment and a rating scale for adherence and compliance evaluation to patients. Clinical internship has favoured the construction of relationship between students of the various disciplines who learned to dialogue together about the centrality of the patient. This experience has favoured a real change in the lifestyle of patients. We hope that this modality can be used also in other UOC involving a raising number of students, tutors, and patients since the modern literature has also concluded that integrated didactic is an inescapable necessity.

10.36017/jahc2005-007

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Citations (0)

Toxoplasma gondii in sheep: Serological occurrence at slaughterhouse level in Italy and environmental risk factors

Frontiers in Veterinary Science (2023)

Roberto Condoleo Pasquale Rombolà Roberta Palumbo Davide Santori Salvatore Serra

Toxoplasmosis is a parasitic disease affecting a wide range of species, including humans, and can be responsible for important clinical manifestations such as abortion and neurological signs. Sheep show a remarkable susceptibility to its causative agent, Toxoplasma gondii, and zoonotic transmission may occur in case of consumption of undercooked meat obtained from infected animals. Toxoplasma gondii seroprevalence in sheep can significantly vary on a geographical basis, as shown by numerous surveys conducted worldwide. To investigate environmental and climate conditions that may affect the likelihood of ovine infection, 405 serum samples from selected sheep raised in 91 farms were collected from two abattoirs, with each abattoir receiving animals from two regions (1/Tuscany-Latium and 2/Campania-Basilicata). The seroprevalence of infection in all examined animals was 53.8%. Young animals (n = 165) had a lower likelihood of being T. gondii positive compared to the adults (OR = 0.21), and the seropositive rate of animals slaughtered in abattoir 2 was significantly higher than that of animals slaughtered in abattoir 1 (60.5 vs. 43.2%, p < 0.01). The significant bioclimatic variables (p < 0.05) associated with the presence of T. gondii antibodies were related to areas with a lower range of temperature and higher precipitation. In conclusion, this study expands on the interpretation of serological data, with the inclusion of environmental and climatic variables, as possible risk factors in the spread of toxoplasmosis in the study area. These findings provide novel insights to support public health measures, such as risk-based control plan, and contribute to a "One Health" approach, taking into account the environmental and climatic perspectives.

10.3389/fvets.2023.1057277

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Citations (5)

Shear Stress Downregulation of Platelet-Derived Growth Factor Receptor-β and Matrix Metalloprotease-2 Is Associated With Inhibition of Smooth Muscle Cell Invasion and Migration

Circulation (2000)

Roberta Palumbo Carlo Gaetano G Melillo Elena Toschi Andrea Remuzzi

After endovascular injury, smooth muscle cells (SMCs) may be exposed to hemodynamic shear stress (SS), and these forces modulate neointima accumulation. The effect of SS on SMC migration and invasion is unknown, and it was examined in the present study.Bovine aortic SMCs were exposed to laminar SS of 12 dyne/cm(2) for 3 (SS3) or 15 (SS15) hours; control (C3 and C15) SMCs were kept under static conditions. Platelet-derived growth factor (PDGF)-BB-directed SMC migration and invasion were evaluated by a modified Boyden chamber assay with filters coated with either gelatin or reconstituted basement membrane proteins (Matrigel), respectively. SS15 inhibited both SMC migration and invasion (P<0.0001). There was no significant difference between SS3 and C3 cells. Media conditioned with SS15 cells exhibited a reduction in matrix metalloprotease-2 (MMP-2) by zymography and Western analysis. Northern blot analysis revealed no effect of SS15 on MMP-2 mRNA. In contrast, SS15 decreased MMP-2 activator and membrane-type MMP (MT-MMP or MMP-14) mRNA and protein. Furthermore, SS15 decreased PDGF receptor-beta (PDGF-Rbeta) mRNA and protein (P<0.05), and the SS-dependent decrease in PDGF-BB-directed cell migration was rescued by overexpressing PDGF-Rbeta.SS inhibits SMC migration and invasion via diminished PDGF-Rbeta expression. This effect of SS is associated with decreased MMP-2 secretion and MT-MMP downregulation.

Platelet-derived growth factor

Matrigel

Neointima

10.1161/01.cir.102.2.225

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Citations (98)

Pacemaker Implantation After Mitral Valve Surgery With Atrial Fibrillation Ablation

Journal of the American College of Cardiology (2019)

Joseph J. DeRose Donna Mancini Helena Chang Michael Argenziano François Dagenais

10.1016/j.jacc.2019.02.062

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Citations (46)

Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-κB activation

The Journal of Experimental Medicine (2007)

Roberta Palumbo Beatriz G. Gálvez Tobias Pusterla Francesco De Marchis Giulio Cossu

HMGB1

10.1084/jem20411oia24

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Citations (2)

Different Effects of High and Low Shear Stress on Platelet-Derived Growth Factor Isoform Release by Endothelial Cells

Arteriosclerosis Thrombosis and Vascular Biology (2002)

Roberta Palumbo Carlo Gaetano Annalisa Antonini Giulio Pompilio Enrico Bracco

In the present study, we analyzed the effect of conditioned media (CM) from bovine aortic endothelial cells exposed to laminar shear stress (SS) of 5 dyne/cm2 (SS5) or 15 dyne/cm2 (SS15) for 16 hours on smooth muscle cell (SMC) migration. In response to CM from bovine aortic endothelial cells exposed to SS5 (CMSS5) and SS15 (CMSS15), migration was 45 +/- 5.5 and 30 +/- 1.5 cells per field, respectively (P<0.05). Similar results were obtained with SS of 2 versus 20 dyne/cm2 and also when SS of 5 and 15 dyne/cm2 lasted 24 hours. Platelet-derived growth factor (PDGF)-AA levels in CMSS5 and CMSS15 were 9 +/- 7 and 18 +/- 5 ng/10(6) cells for 16 hours, respectively (P<0.05); PDGF-BB levels in CMSS5 and CMSS15 were 38 +/- 10 and 53 +/- 10 ng/10(6) cells for 16 hours, respectively (P<0.05). PDGF receptor alpha (PDGFRalpha) and PDGF receptor beta (PDGFRbeta) in SMCs were phosphorylated by CMSS15>CMSS5. In response to CMSS15, a neutralizing antibody against PDGF-AA enhanced SMC migration to a level comparable to that of CMSS5; in contrast, antibodies against PDGF-BB abolished SMC migration. Transfection of SMCs with a dominant-negative PDGFRalpha or PDGFRbeta increased or inhibited, respectively, SMC migration in response to CMSS15. Overexpression of wild-type PDGFRalpha inhibited SMC migration in response to CMSS5, CMSS15, or recombinant PDGF-BB (P<0.001). These results suggest that the ability of high SS to inhibit arterial wall thickening in vivo may be related to enhanced activation of PDGFRalpha in SMCs by PDGF isoforms secreted by the endothelium.

10.1161/hq0302.104528

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Citations (65)