In order to prepare the targeting drugloaded system,magnetic chitosan-5-Fu nanoparticles(MCN-Fu) were prepared by using 5-Fu as a model drug,Fe_3O_4 nanoparticles as the core and glutaraldehyde as cross-linking agent under ultrasonic treatment.TEM images show that MCN-Fu are spherical shape with diameters ranged from 50 nm~ 60 nm.The IR results indicate there is a strong molecular interaction between 5-Fu and chitosan.The drug content,in vitro release and magnetic properties of MCN-Fu were investigated by UV-vis spectrum.The results indicate that the drug content is(21.3%),and accumulative release percentage in phosphate buffer(pH=(7.2)) solution is(67.6%) in 30 h,the drug release rate is found decreased and they can in some degree reduce initial burst.MCN-Fu have good magnetic properties and target effect.
In order to prepare the targeting drug-loaded system,chitosan(CS) 5-fluorouracil((5-Fu))microspheres were prepared in emulsion by using ultrasonic treatment,glutaraldehyde as cross linking agent,span-80 as emulsifer and paraffin oil as oil phase.The condition of preparation has been optimized.The optimum conditions for preparation were as follows: ultrasonic frequency of(40 kHz),2 mL glutaraldehyde,1.0% chitosan and 1.0% 5-fluorouracil.At these conditions,The CS-5-Fu microspheres were characterized by IR,UV-Vis and SEM.The IR results indicate that 5-Fu was loaded.SEM images show that the CS-5-Fu microspheres are spherical shape with diameter's range from 1~ 4 μm.The encapsulated capacity and drug releasing properties were investigated by UVvis spectrum.The results indicate that the drug encapsulation efficiency of Chitosan(CS) 5-fluorouracil(5-Fu) microspheres was 46.7%,and cumulative release percentage in the phosphate buffer(pH = 7.2) solutions was 60.4% in 30 h.
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