Annekathrin Rumlow

Leibniz University Hannover

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Jutta Papenbrock

Leibniz University Hannover

Lourdes Yabor

Universidad de Ciego de Ávila

Anja Riemenschneider

Leibniz University Hannover

Ariel E. Turcios

Leibniz University Hannover

José Carlos Lorenzo

Universidad de Ciego de Ávila

Johann Hornbacher

Leibniz University Hannover

Philip Pallmann

Cardiff University

Marcos Edel Martínez-Montero

Universidad de Ciego de Ávila

Ann Cuypers

Hasselt University

Florent Engelmann

Agropolis International

Cooperative Institutions

Leibniz University Hannover

Universidad de Ciego de Ávila

Universitat Politècnica de València

Hasselt University

Max Planck Institute of Molecular Plant Physiology

Leibniz University of Applied Sciences

Instituto Valenciano de Investigaciones Agrarias

Universidad de Santiago de Chile

Instituto de Biología Molecular y Celular de Plantas

Belgian Nuclear Research Centre

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Quantitative Expression Analysis in Brassica napus by Northern Blot Analysis and Reverse Transcription-Quantitative PCR in a Complex Experimental Setting

PLoS ONE (2016)

Annekathrin Rumlow Els Keunen Jan Klein Philip Pallmann Anja Riemenschneider

Analysis of gene expression is one of the major ways to better understand plant reactions to changes in environmental conditions. The comparison of many different factors influencing plant growth challenges the gene expression analysis for specific gene-targeted experiments, especially with regard to the choice of suitable reference genes. The aim of this study is to compare expression results obtained by Northern blot, semi-quantitative PCR and RT-qPCR, and to identify a reliable set of reference genes for oilseed rape (Brassica napus L.) suitable for comparing gene expression under complex experimental conditions. We investigated the influence of several factors such as sulfur deficiency, different time points during the day, varying light conditions, and their interaction on gene expression in oilseed rape plants. The expression of selected reference genes was indeed influenced under these conditions in different ways. Therefore, a recently developed algorithm, called GrayNorm, was applied to validate a set of reference genes for normalizing results obtained by Northern blot analysis. After careful comparison of the three methods mentioned above, Northern blot analysis seems to be a reliable and cost-effective alternative for gene expression analysis under a complex growth regime. For using this method in a quantitative way a number of references was validated revealing that for our experiment a set of three references provides an appropriate normalization. Semi-quantitative PCR was prone to many handling errors and difficult to control while RT-qPCR was very sensitive to expression fluctuations of the reference genes.

Reference Genes

Northern blot

Normalization

Quantitative Analysis

Southern blot

10.1371/journal.pone.0163679

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Citations (8)

Mineral composition of a transgenic pineapple clone grown in the field for 8 yr

In Vitro Cellular & Developmental Biology - Plant (2017)

Lourdes Yabor Annekathrin Rumlow Daviel Gómez Christoph C. Tebbe Jutta Papenbrock

clone (Java method)

10.1007/s11627-017-9858-x

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Citations (6)

Salinity Influences Single Glucosinolate Content in the Halophyte Lepidium latifolium

Proceedings of the International Plant Sulfur Workshop (2017)

Christian Boestfleisch Johann Hornbacher Annekathrin Rumlow Jutta Papenbrock

Sinigrin

Brassicaceae

10.1007/978-3-319-56526-2_10

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Exposure of Common Bean Seeds to Liquid Nitrogen Modifies Mineral Composition of Young Plantlet Leaves

American Journal of Plant Sciences (2016)

Inaudis Cejas Annekathrin Rumlow Ariel E. Turcios Florent Engelmann Marcos Edel Martínez-Montero

Many publications describe cryopreservation techniques but only a few studies have focused on the biochemical and physiological changes occurring in plants regenerated from seeds exposed to liquid nitrogen. This paper aims at describing the effect of common bean seed cryostorage on mineral nutrition of young plantlets. The following elements were measured on leaves of 10-day-old plantlets from non-cryopreserved and cryopreserved seeds: Al, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, Se, Sr and Zn. At 10 days after sowing, both treatments (control and cryopreserved seeds) showed 100% seed germination without any visual phenotypic difference. However, contents of several elements in the leaves were different. Exposure of seeds to liquid nitrogen decreased Cu, Cd and Na uptake and increased absorption of B and Al. Further studies are required to understand the mechanisms underlying the relationship between seed exposure to liquid nitrogen and mineral nutrition during the early stages of plantlet growth.

Plantlet

Liquid nitrogen

10.4236/ajps.2016.712152

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Citations (6)

The Levels of Sulfur-containing Metabolites in Brassica napus are Not Influenced by the Circadian Clock but Diurnally

Journal of Plant Biology (2019)

Johann Hornbacher Annekathrin Rumlow Philip Pallmann Ariel E. Turcios Anja Riemenschneider

Sulfur Metabolism

Assimilation (phonology)

10.1007/s12374-019-0143-x

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Citations (6)