Mohammad Azhar Aziz

Govind Ballabh Pant University of Agriculture and Technology

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Sameer Mohammad

King Saud bin Abdulaziz University for Health Sciences

Ahmad Deeb

Memorial University of Newfoundland

Zeyad Yousef

King Saud bin Abdulaziz University for Health Sciences

Rakesh Bhatnagar

Jawaharlal Nehru University

Ayman Saleh

Karabük University

A Rizvi

Aligarh Muslim University

Ahmad Aljada

Alfaisal University

Mohammed H AlMuhanna

King Saud bin Abdulaziz University for Health Sciences

Sathish Periyasamy

University of Queensland

M. G. H. Zaidi

Govind Ballabh Pant University of Agriculture and Technology

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King Abdulaziz University

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King Saud bin Abdulaziz University for Health Sciences

King Abdullah International Medical Research Center

National Guard Health Affairs

King Abdulaziz Medical City

King Abdulaziz Hospital

King Saud University

King Abdul Aziz University Hospital

King Faisal Specialist Hospital & Research Centre

Aligarh Muslim University

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Electrochemical remediation of methyl orange over reusable biochar ferrite coated photocatalytic plates

Materials Today Communications (2024)

Diksha Palariya Sameena Mehtab Mohammad Azhar Aziz M. G. H. Zaidi Yasser Vasseghian

Methyl orange

BET theory

10.1016/j.mtcomm.2024.110103

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Multiomics approach towards characterization of tumor cell plasticity and its significance in precision and personalized medicine

Cancer and Metastasis Reviews (2024)

Mohammad Azhar Aziz

Characterization

Personalized Medicine

10.1007/s10555-024-10190-x

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The Role of Improper Use of Artificial Insemination on Infertility and Abortion in Cows in Basrah Province, Iraq: Microbiological Study

H. Mousa Mohammad Azhar Aziz S. S. Bakr N. M. Jamaladdin

دراسة استشرافية أجريت لإظهار المسببات المعدية وطرق انتقالها في حالات العقم والإسقاط في الأبقار. لقد أجرى التلقيح الاصطناعي إلى 434 بقرة ، وقد فشل الحمل في 155 (%35.7 ) حالة منها بعدة عدة محاولات من وكذلك وجد تأخر الدورة التروية بعد الولادة الطبيعية في 398 (99%) من مجموع 402 بقرة. لقد تم عزل عدة أحياء مجهرية معروفة بتسببها للعقم والإسقاط في الأبقار من عينات الرحم والسائل المنوي المجمد ومن أجهزة التلقيح الاصطناعي. تم عزل جرثومة باستوريلا ايروجيتر لمفردها من عينة رحم لبقرة واحدة بعد الإجهاض مباشرة. وكذلك فان هذه الجرثومة تم عزلها من ثلاث عينات من السوائل المنوية المجمدة ومن احدى أجهزة التلقيح الاصطناعي. يستنتج من هذه إن هذه الجرثومة غير المسجلة سابقا بكونها تسبب الإسقاط أو العقم . الدراسة بأن الجراثيم المرضية ممكن أن تنتقل إلى الأبقار السليمة بواسطة أجهزة التلقيح الاصطناعي الملوثة أو بواسطة السائل المنوي الملوث. لذلك فانه يوصي بإجراء زرع جرثومي روتيني وفحص مجهري مباشر للسائل المنوي الذي يستخدم للتلقيح الاصطناعي مع استثناء أو علاج الثيران التي تظهر فيها الإصابة بالأمراض. وكذلك تعقيم أجهزة التلقيح الاصطناعي بضورة روتينية قبل كل استعمال .

Intrauterine insemination

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Presence of Circulatory Autoantibodies Against ROS-Modified Histone H1 Protein in Lymphoma Patients

Frontiers in Genetics (2022)

Naif K. Binsaleh Reem Eltayeb Husam Qanash Mohammad Azhar Aziz Raid Saleem Al-Baradie

Lymphoma is a chronic inflammatory disease in which the immune system is highly affected. Increased oxidative stress is one of the common conditions of cancer and affects macromolecules. Histone modifications affect the chromatin structure and functions. In this study, histone H1 (His-H1) protein was modified by reactive oxygen species (ROS), and structural and chemical changes were studied. Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL) patients were selected, and oxidative stress markers, inflammatory cytokines, and serum autoantibodies were analyzed using biochemical and immunological assays. Furthermore, the formation of antigen-antibody immune complexes was assessed by the Langmuir plot. ROS-modified His-H1 (ROS-His-H1) showed substantial structural perturbation in protein (UV-hyperchromicity and increased intrinsic fluorescence) compared to the native His-H1 protein. A possible explanation for the changes is suggested by the exposure of the aromatic chromophore to the solvent. In-depth structural analysis by circular dichroism (CD) exhibited major changes in α-helix (-21.43%) and turns (+33%), reflecting changes in the secondary structure of histone H1 protein after ROS exposure. ELISA and competitive ELISA findings revealed high recognitions of serum autoantibodies to ROS-His-H1 from NHL, followed by HL subjects. Healthy controls showed negligible binding. Non-modified His-H1 did not show any binding with serum samples from either cohort. High apparent association constants (ACCs) were calculated for ROS-His-H1 using purified IgGs from NHL (1.46 × 10-6 M) compared to HL (1.33 × 10-6 M) patients. Non-modified His-H1 exhibited a hundred times less ACC for NHL (2.38 × 10-8 M) and HL (2.46 × 10-8 M) patients. Thus, ROS modifications of histone H1 cause structural changes and expose cryptic neo-epitopes on the protein against which autoantibodies were generated. These perturbations might affect the histone DNA interaction dynamics and potentially be correlated with gene dysregulation. These subtle molecular changes with an immune imbalance might further aggravate the disease.

10.3389/fgene.2022.909903

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A Model of Exposure to Extreme Environmental Heat Uncovers the Human Transcriptome to Heat Stress

Scientific Reports (2017)

Abderrezak Bouchama Mohammad Azhar Aziz Saeed Al Mahri Musa Gabere Meshan Al Dlamy

The molecular mechanisms by which individuals subjected to environmental heat stress either recover or develop heat-related complications are not well understood. We analysed the changes in blood mononuclear gene expression patterns in human volunteers exposed to extreme heat in a sauna (temperature of 75.7 ± 0.86 °C). Our analysis reveals that expression changes occur rapidly with no significant increase in core temperature and continue to amplify one hour after the end of heat stress. The reprogramed transcriptome was predominantly inhibitory, as more than two-thirds of the expressed genes were suppressed. The differentially expressed genes encoded proteins that function in stress-associated pathways; including proteostasis, energy metabolism, cell growth and proliferation, and cell death, and survival. The transcriptome also included mitochondrial dysfunction, altered protein synthesis, and reduced expression of genes -related to immune function. The findings reveal the human transcriptomic response to heat and highlight changes that might underlie the health outcomes observed during heat waves.

Proteostasis

Extreme heat

10.1038/s41598-017-09819-5

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Towards sustainable and selective detection of lead Ions: Selaginella bryopteris assisted green synthesis of gold nanoparticles with tunable properties

Journal of environmental chemical engineering (2023)

A Rizvi Mohammad Azhar Aziz Alim H. Naqvi Absar Ahmad

10.1016/j.jece.2023.110757

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Modulation of ATP8B1 Gene expression in Colorectal Cancer Cells Suggest its Role as a Tumor Suppressor

Saleh Althenayyan Amal AlGhamdi Mohammed H AlMuhanna Esraa Hawsa Dalal Aldeghaither

Sporadic colorectal cancer (CRC) develops through distinct molecular events. Loss of 18q chromosome is a conspicuous event in the progression of adenoma to carcinoma. There is limited information regarding the molecular effectors of this event. Earlier, we had reported ATP8B1 as a novel gene associated with CRC. ATP8B1 belongs to the family of P-type ATPases (P4 ATPase) that primarily function to facilitate the translocation of phospholipids. In this study, we attempt to implicate ATP8B1 gene located on chromosome 18q as a tumor suppressor gene. We studied indigenous patient data and confirmed the reduced expression of ATP8B1 in tumor samples. CRC cell lines were engineered with reduced and enhanced levels of ATP8B1 which provided a tool to study its role on cancer progression. Forced reduction of ATP8B1 expression either by CRISPR/Cas9 or shRNA was associated with increased growth and proliferation of CRC cell line - HT29. In contrast, overexpression of ATP8B1 resulted in reduced growth and proliferation of SW480 cell line. We generated a network of genes that are downstream of ATP8B1. Further, we provide predicted effect of modulation of ATP8B1 levels on this network and possible effect on fatty acid metabolism related genes. These results provide evidence in support of ATP8B1 being a tumor suppressor that may affect fatty acid metabolism in CRC.

10.20944/preprints202011.0437.v1

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Correction: Novel Genes Associated with Colorectal Cancer Are Revealed by High Resolution Cytogenetic Analysis in a Patient Specific Manner

PLoS ONE (2013)

Hisham Eldai Sathish Periyasamy Saeed Al Qarni Maha Al Rodayyan Sabeena Mustafa

Genomic abnormalities leading to colorectal cancer (CRC) include somatic events causing copy number aberrations (CNAs) as well as copy neutral manifestations such as loss of heterozygosity (LOH) and uniparental disomy (UPD).We studied the causal effect of these events by analyzing high resolution cytogenetic microarray data of 15 tumor-normal paired samples.We detected 144 genes affected by CNAs.A subset of 91 genes are known to be CRC related yet high GISTIC scores indicate 24 genes on chromosomes 7, 8, 18 and 20 to be strongly relevant.Combining GISTIC ranking with functional analyses and degree of loss/gain we identify three genes in regions of significant loss (ATP8B1, NARS, and ATP5A1) and eight in regions of gain (CTCFL, SPO11, ZNF217, PLEKHA8, HOXA3, GPNMB, IGF2BP3 and PCAT1) as novel in their association with CRC.Pathway and target prediction analysis of CNA affected genes and microRNAs, respectively indicates TGF-b signaling pathway to be involved in causing CRC.Finally, LOH and UPD collectively affected nine cancer related genes.Transcription factor binding sites on regions of .35%copy number loss/gain influenced 16 CRC genes.Our analysis shows patient specific CRC manifestations at the genomic level and that these different events affect individual CRC patients differently.

Uniparental disomy

10.1371/annotation/3f97e271-6926-4766-8430-1b4a009e80c6

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Computational identification of PDL1 inhibitors and their cytotoxic effects with silver and gold nanoparticles

Research Square (Research Square) (2024)

Syed Hammad Ali Hiba Ali Mohammad Azhar Aziz

Abstract Background Immunotherapy is a promising treatment for cancer that aims to boost the immune system's response to cancer cells. This can be achieved by blocking PD1(Programmed cell death 1)/PDL1(Programmed death-ligand 1), which activates T cells. In this work, the aim was to find high-affinity drugs against PDL1 using computational tools and conjugate them with nanoparticles. The cytotoxic activity of the drug-conjugated nanoparticles was then tested. Methods The screening of one hundred thousand drugs from the ZINC database and FDA-approved drugs was done computationally. The physicochemical properties and toxicity of the drugs were analyzed using SwissADME and ProTox-II respectively. AgNPs and AuNPs were synthesized using extracts of Catharanthus roseus flowers and Juglans regia shells, respectively. The characterization of AgNPs and AuNPs was performed using UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR). Their conjugation with the drugs Irinotecan, Imatinib, and Methotrexate was also confirmed using UV-Vis, FTIR, and Dynamic light scattering (DLS). Results The top screened drugs were ZINC1098661 and three FDA-approved drugs (Irinotecan, Imatinib, and Methotrexate). Docking studies revealed that Irinotecan had the highest binding affinity towards PDL1 when conjugated with silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs). The Irinotecan-PDL1 complex was confirmed as the most stable through molecular dynamics simulations. The result of methylthiazol tetrazolium (MTT) assay showed that conjugated AgNPs and AuNPs with Irinotecan had a high toxic effect on A549 cancer cell line than Imatinib conjugated with AgNPs and AuNPs. Conclusion: This study provides a promising avenue for further investigation and development of nanoparticle-drug conjugates as a potential cancer immunotherapy strategy.

Conjugate

MTT assay

10.21203/rs.3.rs-4704476/v1

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<p>Transcriptomics-Based Characterization of the Toxicity of ZnO Nanoparticles Against Chronic Myeloid Leukemia Cells</p>

International Journal of Nanomedicine (2020)

Suliman A. Alsagaby Rajendran Vijayakumar Mariappan Premanathan Suresh Mickymaray Wael Alturaiki

Introduction: Zinc oxide nanoparticles (ZnO NPs) have recently attracted attention as potential anti-cancer agents. To the best of our knowledge, the toxicity of ZnO NPs against human chronic myeloid leukemia cells (K562 cell line) has not been studied using transcriptomics approach. Objective: The goals of this study were to evaluate the capability of ZnO NPs to induce apoptosis in human chronic myeloid leukemia cells (K562 cells) and to investigate the putative mechanisms of action. Methods: We used viability assay and flowcytometry coupled with Annexin V-FITC and propidium iodide to investigate the toxicity of ZnO NPs on K562 cells and normal peripheral blood mononuclear cells. Next we utilized a DNA microarray-based transcriptomics approach to characterize the ZnO NPs-induced changes in the transcriptome of K562 cells. Results: ZnO NPs exerted a selective toxicity (mainly by apoptosis) on the leukemic cells ( p ≤ 0.005) and altered their transcriptome; 429 differentially expressed genes (DEGs) with fold change (FC)≥ 4 and p ≤ 0.008 with corrected p ≤ 0.05 were identified in K562 cells post treatment with ZnO NPs. The over-expressed genes were implicated in "response to zinc", "response to toxic substance" and "negative regulation of growth" (corrected p ≤ 0.05). In contrast, the repressed genes positively regulated "cell proliferation", "cell migration", "cell adhesion", "receptor signaling pathway via JAK-STAT" and "phosphatidylinositol 3-kinase signaling" (corrected p ≤ 0.05). Lowering the FC to ≥ 1.5 with p ≤ 0.05 and corrected p ≤ 0.1 showed that ZnO NPs over-expressed the anti-oxidant defense system, drove K562 cells to undergo mitochondrial-dependent apoptosis, and targeted NF-κB pathway. Conclusion: Taken together, our findings support the earlier studies that reported anti-cancer activity of ZnO NPs and revealed possible molecular mechanisms employed by ZnO NPs to induce apoptosis in K562 cells. Keywords: transcriptomics, ZnO NPs, CML, apoptosis

K562 cells

Propidium iodide

10.2147/ijn.s261636

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Citations (32)