Jędrzej Szymański

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

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Jerzy Duszyński

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Robert Hołyst

Institute of Physical Chemistry

Bernadeta Michalska

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Paulina Patalas-Krawczyk

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Mariusz R. Więckowski

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Tomasz Kalwarczyk

Institute of Physical Chemistry

Natalia Nowak

Polish Academy of Sciences

Joanna Szczepanowska

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Agnieszka Wilk

AGH University of Krakow

Jarosław Walczak

Institute of Fundamental Technological Research

Cooperative Institutions

Polish Academy of Sciences

University of Warsaw

Institute of Physical Chemistry

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Institute of Physics

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Philip Morris International (Switzerland)

European Molecular Biology Laboratory

University of Ferrara

Warsaw University of Technology

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Comparative Analysis of Viscosity of Complex Liquids and Cytoplasm of Mammalian Cells at the Nanoscale

Nano Letters (2011)

Tomasz Kalwarczyk Natalia Ziębacz Anna Bielejewska Ewa Zaboklicka Kaloian Koynov

We present a scaling formula for size-dependent viscosity coefficients for proteins, polymers, and fluorescent dyes diffusing in complex liquids. The formula was used to analyze the mobilities of probes of different sizes in HeLa and Swiss 3T3 mammalian cells. This analysis unveils in the cytoplasm two length scales: (i) the correlation length ξ (approximately 5 nm in HeLa and 7 nm in Swiss 3T3 cells) and (ii) the limiting length scale that marks the crossover between nano- and macroscale viscosity (approximately 86 nm in HeLa and 30 nm in Swiss 3T3 cells). During motion, probes smaller than ξ experienced matrix viscosity: η(matrix) ≈ 2.0 mPa·s for HeLa and 0.88 mPa·s for Swiss 3T3 cells. Probes much larger than the limiting length scale experienced macroscopic viscosity, η(macro) ≈ 4.4 × 10(-2) and 2.4 × 10(-2) Pa·s for HeLa and Swiss 3T3 cells, respectively. Our results are persistent for the lengths scales from 0.14 nm to a few hundred nanometers.

HeLa

Nanometre

Length scale

Limiting

3T3 cells

10.1021/nl2008218

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Citations (235)

Distinction of sporadic and familial forms of ALS based on mitochondrial characteristics

The FASEB Journal (2018)

Jarosław Walczak Grażyna Dębska‐Vielhaber Stefan Vielhaber Jędrzej Szymański Agata Charzyńska

Bioenergetic failure, oxidative stress, and changes in mitochondrial morphology are common pathologic hallmarks of amyotrophic lateral sclerosis (ALS) in several cellular and animal models. Disturbed mitochondrial physiology has serious consequences for proper functioning of the cell, leading to the chronic mitochondrial stress. Mitochondria, being in the center of cellular metabolism, play a pivotal role in adaptation to stress conditions. We found that mitochondrial dysfunction and adaptation processes differ in primary fibroblasts derived from patients diagnosed with either sporadic or familial forms of ALS. The evaluation of mitochondrial parameters such as the mitochondrial membrane potential, the oxygen consumption rate, the activity and levels of respiratory chain complexes, and the levels of ATP, reactive oxygen species, and Ca2+ show that the bioenergetic properties of mitochondria are different in sporadic ALS, familial ALS, and control groups. Comparative statistical analysis of the data set (with use of principal component analysis and support vector machine) identifies and distinguishes 3 separate groups despite the small number of investigated cell lines and high variability in measured parameters. These findings could be a first step in development of a new tool for predicting sporadic and familial forms of ALS and could contribute to knowledge of its pathophysiology.—Walczak, J., Dębska-Vielhaber, G., Vielhaber, S., Szymański, J., Charzyńska, A., Duszyński, J., Szczepanowska, J. Distinction of sporadic and familial forms of ALS based on mitochondrial characteristics. FASEB J. 33, 4388–4403 (2019). www.fasebj.org

Bioenergetics

Mitochondrial disease

10.1096/fj.201801843r

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Citations (28)

Eine Methode zur Untersuchung der Ruhe-und Aktivitätsperioden bei Tieren

Pflügers Archiv - European Journal of Physiology (1914)

Jędrzej Szymański

10.1007/bf01703428

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Citations (76)

Aggregation of aqueous lysozyme solutions followed by dynamic light scattering and 1H NMR spectroscopy

Journal of Molecular Liquids (2004)

Jarosław Poznański Jędrzej Szymański Teresa Basiǹska Stanisław Słomkowski Wojciech Zielenkiewicz

Rotational diffusion

10.1016/j.molliq.2004.08.022

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Citations (26)

VI. Die Denkformen.

Key issues in mental health (Online)/Key issues in mental health (Print) (2015)

Jędrzej Szymański

10.1159/000396457

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Minimal Tags for Rapid Dual‐Color Live‐Cell Labeling and Super‐Resolution Microscopy

Angewandte Chemie International Edition (2014)

Ivana Nikić Tilman Plass Oliver Schraidt Jędrzej Szymański John A. G. Briggs

Abstract The growing demands of advanced fluorescence and super‐resolution microscopy benefit from the development of small and highly photostable fluorescent probes. Techniques developed to expand the genetic code permit the residue‐specific encoding of unnatural amino acids (UAAs) armed with novel clickable chemical handles into proteins in living cells. Here we present the design of new UAAs bearing strained alkene side chains that have improved biocompatibility and stability for the attachment of tetrazine‐functionalized organic dyes by the inverse‐electron‐demand Diels–Alder cycloaddition (SPIEDAC). Furthermore, we fine‐tuned the SPIEDAC click reaction to obtain an orthogonal variant for rapid protein labeling which we termed selectivity enhanced (se) SPIEDAC. seSPIEDAC and SPIEDAC were combined for the rapid labeling of live mammalian cells with two different fluorescent probes. We demonstrate the strength of our method by visualizing insulin receptors (IRs) and virus‐like particles (VLPs) with dual‐color super‐resolution microscopy.

Tetrazine

Bioorthogonal Chemistry

Protein tag

Genetic Code

Live cell imaging

10.1002/anie.201309847

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Citations (288)

Title Page / Vorwort / Inhaltsverzeichnis

Key issues in mental health (Online)/Key issues in mental health (Print) (2015)

Jędrzej Szymański

10.1159/000396452

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Citations (0)

Scaling form of viscosity at all length-scales in poly(ethylene glycol) solutions studied by fluorescence correlation spectroscopy and capillary electrophoresis

Physical Chemistry Chemical Physics (2009)

Robert Hołyst Anna Bielejewska Jędrzej Szymański Agnieszka Wilk A. Patkowski

We measured the viscosity of poly(ethylene glycol) (PEG 6000, 12 000, 20 000) in water using capillary electrophoresis and fluorescence correlation spectroscopy with nanoscopic probes of different diameters (from 1.7 to 114 nm). For a probe of diameter smaller than the radius of gyration of PEG (e.g.rhodamine B or lyzozyme) the measured nanoviscosity was orders of magnitude smaller than the macroviscosity. For sizes equal to (or larger than) the polymer radius of gyration, macroscopic value of viscosity was measured. A mathematical relation for macro and nanoviscosity was found as a function of PEG radius of gyration, Rg, correlation length in semi-dilute solution, ξ, and probe size, R. For R < Rg, the nanoviscosity (normalized by water viscosity) is given by exp(b(R/ξ)a), and for R > Rg, both nano and macroviscosity follow the same curve, exp(b(R/ξ)a), where a and b are two constants close to unity. This mathematical relation was shown to equally well describe rhodamine (of size 1.7 nm) in PEG 20 000 and the macroviscosity of PEG 8 000 000, whose radius of gyration exceeds 200 nm. Additionally, for the smallest probes (rhodamine B and lysozyme) we have verified, using capillary electrophoresis and fluorescence correlation spectroscopy, that the Stokes–Einstein (SE) relation holds, providing that we use a size-dependent viscosity in the formula. The SE relation is correct even in PEG solutions of very high viscosity (three orders of magnitude larger than that of water).

Radius of gyration

Fluorescence Correlation Spectroscopy

Hydrodynamic radius

Rhodamine B

Rhodamine

Gyration

10.1039/b908386c

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Citations (184)

Herbal compounds influence on refractive index of neuroblastoma cells based on holographic tomography measurements

Digital Holography and 3-D Imaging 2022 (2022)

Maria Baczewska Natalia Nowak Jędrzej Szymański Chau-Jern Cheng Małgorzata Kujawińska

Yokukansan (SNY) is a traditional Japanese herbal medicine that is becoming more widely researched for effects on neurodegenerative diseases. In our research we extend the knowledge about the effects of SNY on neuronal cells and we demonstrated for the first time that the effect of different doses of SNY on cell culture can be observed by holographic tomography.

10.1364/dh.2022.m2a.4

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Micro- and macro-shear viscosity in dispersed lamellar phases

Journal of Non-Newtonian Fluid Mechanics (2007)

Jędrzej Szymański Agnieszka Wilk Robert Hołyst G.P. Roberts Kirsty Sinclair

Lamellar phase

Reduced viscosity

Fluorescence Correlation Spectroscopy

10.1016/j.jnnfm.2007.04.008

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Citations (12)