Sylwia Budniak

National Veterinary Research Institute

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Agnieszka Kędrak-Jabłońska

National Veterinary Research Institute

M. Reksa

National Veterinary Research Institute

Anna Szczawińska

National Veterinary Research Institute

Marek Krupa

National Veterinary Research Institute

Krzysztof Szulowski

National Veterinary Research Institute

K. Szulowski

National Veterinary Research Institute

Viviana Manzulli

Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata

Erika Matuschek

Växjö Kommun

Mandy C. Elschner

Friedrich-Loeffler-Institut

Daniela Jacob

Robert Koch Institute

Cooperative Institutions

National Veterinary Research Institute

Universität der Bundeswehr München

Institut für Mikrobiologie der Bundeswehr

National Veterinary Research Institute

Czech Academy of Sciences, Institute of Microbiology

German Center for Infection Research

Norwegian Institute of Public Health

Bundeswehr Medical Service Academy

Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata

Public Health Agency of Sweden

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Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method

Microorganisms (2022)

Alina Tscherne Enrico Mantel Talar Boskani Sylwia Budniak Mandy C. Elschner

Brucellosis, mainly caused by

10.3390/microorganisms10071470

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Citations (9)

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Journal of Veterinary Research (2016)

Sylwia Budniak Agnieszka Kędrak-Jabłońska Anna Szczawińska M. Reksa Marek Krupa

Abstract Introduction: The aim of the study was to optimise and compare two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples including the liver, brain, and blood. Material and Methods: Three strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used. Additionally, five other species of bacterium were used to evaluate the specificity of the tests. Results: Specific amplification products were obtained for both multiplex PCR assays, which confirmed the tested strains as Listeria spp. and L. monocytogenes, respectively. Isolates of other species did not yield PCR products. Conclusion: Both multiplex PCR assays proved to be significantly sensitive and highly-specific methods for the detection of Listeria strains.

Multiplex

10.1515/jvetres-2016-0061

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Citations (5)

Isolation and identification of Pasteurella multocida from European bison in Poland

Postępy Mikrobiologii. Suplement (2017)

Agnieszka Kędrak-Jabłońska Sylwia Budniak Anna Szczawińska M. Reksa Marek Krupa

Isolation

Identification

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Citations (3)

Evaluation of Real-Time Pcr Based on Sybr Green I Fluorescent Dye for Detection of Bacillus Anthracis Strains in Biological Samples

Journal of Veterinary Research (2018)

Agnieszka Kędrak-Jabłońska Sylwia Budniak Anna Szczawińska M. Reksa Marek Krupa

Infection of goats with caprine arthritis encephalitis virus (CAEV) has been detected in variable proportions in many countries all over the world. Here, we investigated the seroprevalence of CAEV in goats raised in Algeria.A serological survey was performed on serum samples from 1,313 goats, including the local breeds (Arabia and Dwarf of Kabylia) and imported European breeds (Alpine and Saanen). Blood samples were taken from goats on 38 farms distributed across four different geographical regions of Algeria. Serum samples were tested for CAEV antibodies using a commercial ELISA.A total of 390 serum samples were found to be positive for CAEV, giving an overall seropositivity rate of 29.7% in individual animals and 97.37% (37/38) at the goat farm level.These results provide the first large-scale serological evidence for the presence of CAEV infection in both the local and imported breeds of goats raised in Algeria, indicating that the virus infection is widespread.

Seroprevalence

SYBR Green I

10.2478/jvetres-2019-0001

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Citations (6)

Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

Journal of Veterinary Research (2017)

Agnieszka Kędrak-Jabłońska Sylwia Budniak Marek Krupa Anna Szczawińska M. Reksa

Abstract Introduction: The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hly A gene of Listeria monocytogenes in biological samples of the liver, brain, and blood. Material and Methods: Five strains of L. monocytogenes and single strains of each species L. ivanovii , L. innocua , L. grayi , L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of tests. In the first stage of the study SYBR Green I real-time PCRs, one allowing detection of the 23S rDNA gene and two based on the amplification the hly A gene, were performed. In the next part, three TaqMan probe-based real-time PCRs allowing confirmation of belonging to Listeria spp. and L. monocytogenes were conducted. Results: The observation of amplification curves in real-time PCRs enabled the detection of both genes. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hly A genes which confirm their belonging to Listeria spp. and L. monocytogenes , respectively. Other microbial species did not reveal real-time PCR products. Conclusion: Both real-time PCR methods for the detection of Listeria spp. and L. monocytogenes in biological samples demonstrated a significant sensitivity and high specificity.

TaqMan

SYBR Green I

10.1515/jvetres-2017-0069

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Citations (10)

Application of the Real-time PCR method with TaqMan probes for the detection of Bacillus anthracis virulence marker – gene pag and specific chromosomal sequence rpo B

Postępy Nauk Medycznych (2011)

Sylwia Budniak Agnieszka Kędrak-Jabłońska M. Reksa Anna Szczawińska Marek Krupa

TaqMan

Bacillus anthracis

Sequence (biology)

Source

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Citations (0)

Studies on the application of the western-blotting method for serological diagnostics of glanders.

Medycyna Weterynaryjna (2013)

Agnieszka Kędrak-Jabłońska Sylwia Budniak Anna Szczawińska M. Reksa Marek Krupa

Source

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European multi-center study to establish MIC and zone diameter epidemiological cut-off (ECOFF) values for Bacillus anthracis

Clinical Microbiology and Infection (2024)

Flavia Dematheis Viviana Manzulli Gregor Grass Erika Matuschek Daniela Jacob

Bacillus anthracis clinical breakpoints, representing a systematic approach to guide clinicians in selecting the most appropriate antimicrobial treatments, are not part of the guidance from the European Committee on Antimicrobial Susceptibility Testing (EUCAST). This is because defined distributions of MIC values and of epidemiological cut-off values (ECOFFs) have been lacking. In this study, a Europe-wide network of laboratories in collaboration with EUCAST, aimed at establishing standardized antimicrobial susceptibility testing methods, wild-type MIC distributions, and ECOFFs for ten therapeutically relevant antimicrobials.

Broth microdilution

Bacillus anthracis

10.1016/j.cmi.2024.05.019

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Citations (0)

Pastereloza królików - aktualny stan wiedzy

Medycyna Weterynaryjna (2012)

Sylwia Budniak Agnieszka Kędrak-Jabłońska K. Szulowski Anna Szczawińska M. Reksa

Summary Pasteurellosis in rabbits is caused by the bacterium Pasteurella multocida. The serotypes 3 or 12 strains of P. multocida, representing diverse virulence and pathogenicity, are most frequently separated from rabbits. P. multocida causes a variety of clinical syndromes, including acute and chronic rhinitis, pneumonia, otitis media, infections of the genital tract, localized abscesses and septicemia. The fight against pasteurellosis is based on two strategies: elimination of carriers and sick animals from breeding and the improvement of environmental conditions. As the disease is caused by various serotypes, vaccination becomes difficult. Immunity against one serotype does not necessarily result in protection against a heterologous serotype. A detailed description of the ethiopatology, clinical and anatomopathological manifestations, diagnosis, therapy and control of the disease has been presented in the paper.

Pasteurellosis

Heterologous

Pasteurella

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Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

Bulletin of the Veterinary Institute in Pulawy (2015)

Agnieszka Kędrak-Jabłońska Sylwia Budniak Anna Szczawińska M. Reksa Marek Krupa

Abstract The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.

TaqMan

SYBR Green I

10.1515/bvip-2015-0073

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Citations (3)