Cuiqing Zhong

UPMC Hillman Cancer Center

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Jeremy N. Rich

University of Pittsburgh Medical Center

Ryan C. Gimple

Washington University in St. Louis

Briana C. Prager

Cleveland Clinic Lerner College of Medicine

Graham McVicker

Salk Institute for Biological Studies

Alex E. Clark

University of California, San Diego

Arko Sen

University of Alabama at Birmingham

Zhe Zhu

Columbia University Irving Medical Center

Jair L. Siqueira-Neto

University of California, San Diego

Sungjun Beck

University of California, San Diego

Jean Bernatchez

Center for Discovery

Cooperative Institutions

Chinese Academy of Sciences

Salk Institute for Biological Studies

University of Chinese Academy of Sciences

Shanghai Jiao Tong University

University of California, San Diego

Center for Excellence in Molecular Cell Science

Peking University

Fudan University

Altos Labs

Chinese Academy of Medical Sciences & Peking Union Medical College

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Efficient Generation of Gene-Modified Mice by Haploid Embryonic Stem Cell-Mediated Semi-cloned Technology

Methods in molecular biology (2016)

Cuiqing Zhong Jinsong Li

Pronucleus

Gene targeting

Cloning (programming)

Gene knockout

10.1007/978-1-4939-6472-7_8

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Citations (17)

Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells

Cell (2012)

Hui Yang Linyu Shi Bang-An Wang Dan Liang Cuiqing Zhong

Parthenogenesis

Gamete

10.1016/j.cell.2012.04.002

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Citations (182)

Supplementary Table from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Arko Sen Briana C. Prager Cuiqing Zhong Donglim Park Zhe Zhu

Supplementary Table from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Table (database)

Expression (computer science)

10.1158/0008-5472.22431454

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Supplementary Table from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Arko Sen Briana C. Prager Cuiqing Zhong Donglim Park Zhe Zhu

Supplementary Table from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Table (database)

10.1158/0008-5472.22431442

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Transient exposure to miR‐203 enhances the differentiation capacity of established pluripotent stem cells

The EMBO Journal (2020)

María Salazar‐Roa Marianna Trakala Mónica Álvarez‐Fernández Fátima Valdés‐Mora Cuiqing Zhong

10.15252/embj.2019104324

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Citations (20)

A novel microRNA-based strategy to expand the differentiation potency of stem cells

bioRxiv (Cold Spring Harbor Laboratory) (2019)

María Salazar‐Roa Marianna Trakala Mónica Álvarez‐Fernández Fátima Valdés‐Mora Cuiqing Zhong

SUMMARY Full differentiation potential along with self-renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro. We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already-established murine and human PSCs. Short exposure to miR-203 in PSCs ( mi PSCs) results in expanded differentiation potency as well as improved efficiency in stringent assays such as tetraploid complementation and human-mouse interspecies chimerism. Mechanistically, these effects are mediated by direct repression of de novo DNA methyltransferases Dnmt3a and Dnmt3b, leading to transient and reversible erasing of DNA methylation. As a proof of concept, miR-203 improves differentiation and maturation of PSCs into cardiomyocytes in vitro as well as cardiac regeneration in vivo, after cardiac injury. These data support the use of transient exposure to miR-203 as a general and single method to reset the epigenetic memory in PSCs, and improve their use in regenerative medicine.

10.1101/826446

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Supplementary Table from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Arko Sen Briana C. Prager Cuiqing Zhong Donglim Park Zhe Zhu

Supplementary Table from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Table (database)

Expression (computer science)

10.1158/0008-5472.22431451.v1

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CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library

Cell stem cell (2015)

Cuiqing Zhong Yin Qi Zhenfei Xie Meizhu Bai Rui Dong

10.1016/j.stem.2015.07.015

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Citations (6)

αKLOTHO and sTGFβR2 treatment counteract the osteoarthritic phenotype developed in a rat model

Protein & Cell (2020)

Paloma Martínez‐Redondo Isabel Guillen-Guillen Noah Davidsohn Chao Wang Javier Prieto

Klotho

Human genetics

Developmental Biology

10.1007/s13238-019-00685-7

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Citations (18)

Data from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma

Arko Sen Briana C. Prager Cuiqing Zhong Donglim Park Zhe Zhu

<div>AbstractGlioblastoma is the most prevalent primary malignant brain tumor in adults and is characterized by poor prognosis and universal tumor recurrence. Effective glioblastoma treatments are lacking, in part due to somatic mutations and epigenetic reprogramming that alter gene expression and confer drug resistance. To investigate recurrently dysregulated genes in glioblastoma, we interrogated allele-specific expression (ASE), the difference in expression between two alleles of a gene, in glioblastoma stem cells (GSC) derived from 43 patients. A total of 118 genes were found with recurrent ASE preferentially in GSCs compared with normal tissues. These genes were enriched for apoptotic regulators, including schlafen family member 11 (SLFN11). Loss of SLFN11 gene expression was associated with aberrant promoter methylation and conferred resistance to chemotherapy and PARP inhibition. Conversely, low SLFN11 expression rendered GSCs susceptible to the oncolytic flavivirus Zika. This discovery effort based upon ASE revealed novel points of vulnerability in GSCs, suggesting a potential alternative treatment strategy for chemotherapy-resistant glioblastoma.Significance:Assessing allele-specific expression reveals genes with recurrent cis-regulatory changes that are enriched in glioblastoma stem cells, including SLFN11, which modulates chemotherapy resistance and susceptibility to the oncolytic Zika virus.</div>

10.1158/0008-5472.c.6513907.v1

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