Krzysztof Jasik

Medical University of Silesia

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Alicja Buczek

Medical University of Lublin

Hubert Okła

University of Silesia in Katowice

Marta Albertyńska

Wojewódzki Szpital Specjalistyczny Nr 2

Beata Rozwadowska

Wojewódzki Szpital Specjalistyczny Nr 2

L Buczek

Medical University of Lublin

Jan Słodki

Medical University of Silesia

Anna Kleczka

Medical University of Silesia

Danuta Urbańska-Jasik

University of Silesia in Katowice

Robert D. Wojtyczka

Medical University of Silesia

Andrzej Swinarew

University of Silesia in Katowice

Cooperative Institutions

Medical University of Silesia

University of Silesia in Katowice

Górnośląskie Centrum Medyczne

Institute of Occupational Medicine and Environmental Health

Silesian Center for Heart Disease

Wojewódzki Szpital Specjalistyczny nr 5 im. św. Barbary w Sosnowcu

Jagiellonian University

University of Economics in Katowice

Polish Academy of Sciences

Institute of Physics

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Snail mucus - a natural origin substance with potential use in medicine

Acta Poloniae Pharmaceutica - Drug Research (2022)

Ewa Waluga Krzysztof Jasik Dominika Wcisło‐Dziadecka P Pol Kornelia Kuźnik‐Trocha

Natural origin substances with healing properties of potential use in the treatment of different diseases, particularly wound management, remain in the scientific and research interest for many years.The substance of natural origin with potentially wide use in medicine and pharmacy is snail mucus.The study aims to evaluate the effect of snail mucus extract on human fibroblasts and evaluate the biological and cytotoxic activity of this substance.In the present study, human NHDF fibroblast cells were exposed to snail slime at two different concentrations: 15 µL/mL and 70 µL/mL.Moreover, in this study, the assessment of the biological activity of cells was conducted using mitochondrial redox activity.At the same time, the analysis of the mucus sample was carried out throughout the infrared spectroscopy implementation.It has been shown that this substance contains compounds such as mucin, achacin, mitomycin, allantoin.The snail mucus affects the viability of fibroblasts -connective tissue cells found in the dermis, responsible for collagen and elastin production.The mechanism of positive action on human fibroblasts is not entirely known and requires further research.

10.32383/appdr/145377

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Citations (8)

Analysis of the polymorphism of Staphylococcus strains isolated from a hospital environment

African Journal of Microbiology Research (2011)

Robert D. Wojtyczka Daniel Krakowian Lukasz Marek Dominik Skiba Adam Kudelski

Nosocomial infections, despite the introduction of the numerous control systems, still constitute a considerable problem in hospitals. They are of great socio-economic importance due to the prolonged stay of patients in hospitals and high treatment costs. They also contribute to an increase in the death rate. For many years staphylococci have remained at the top of the list of microorganisms that cause infections in hospitals. The methicillin-resistant staphylococci (MRSA) strains that occur among both coagulase positive and coagulase negative (MRCNS) species are particularly dangerous. The aim of the presented paper was to determine the usefulness of the 16S rRNA gene sequence analysis and the region between the genes coding 16 S and 23 S rRNA for an examination of the diversity of Staphylococcus spp. isolated from a hospital environment and to perform a polymorphism analysis within and among the species. We also analyzed the polymorphism of 84 Staphylococcus strains and isolated from a hospital environment using PCR-RFLP and ITS PCR methods. A restriction enzyme analysis of the 16 S rRNA gene using TaqI enzyme did not show any considerable power of differentiation in relation to the Staphylococci strains examined. However, the variable region between genes 16 S and 23S rRNA showed a high degree of polymorphisms and allowed for the differentiation of the hospital strains examined. The results demonstrated the highest differentiation inStaphylococcus aureus strains. The examined strains originating from hospital environment are characterised by a high polymorphism. Key words: ITS-PCR, Staphylococcus spp., hospital infections, 16 S rRNA, 23 S rRNA.

10.5897/ajmr11.595

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Citations (2)

Expression of Genes Associated with H Factor in Fibroblasts Infected with Borrelia Spirochaetes

Scandinavian Journal of Immunology (2012)

Aleksandra Gęca Joanna Gola Sławomir Dudek Krzysztof Jasik Małgorzata Muc‐Wierzgoń

Abstract Infection with Borrelia spirochaetes leads to the launch of specific and non‐specific immunological response in humans. Activation of the complement system is one of the first defence mechanisms against penetrating pathogens. The aim of this study was to select genes related to the alternative pathway of the complement system [including complement factor H (CFH)], differentiating the type of infection in the system model, that is, a culture of normal human diploid fibroblasts infected with three different spirochaete genospecies: Borrelia afzelii, Borrelia garinii and Borrelia burgdorferii sensu stricto, by comparing the infected fibroblast culture with the control fibroblast one. With the use of oligonucleotide microarrays HGU‐133A, the differences in the expression of genes selected on the basis of a scientific database Affymetrix were studied by comparing transcriptomes from the four cultures of fibroblasts. In the result of infection of fibroblast cultivation with a specific Borrelia genospecies, a variable expression of certain CFH and complement system‐associated genes, specific for one genospecies only, B. afzelii – C1QBP, CD59, C2, CD46 and FHL1; B. garinii – C1S and CLU; Borrelia burgeforii – CFB, A2M and VSIG4, was observed. CFH differentiates infections induced by B. afzelii and B. garinii from infections induced by B. burgdorferii sensu stricto .

Borrelia afzelii

Borrelia garinii

Spirochaete

10.1111/j.1365-3083.2012.02741.x

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Citations (3)

NiTi Shape Memory Clamps with Modified Surface for Bone Fracture Treatment

Materials (2023)

Tomasz Goryczka Tomasz Szponder Karolina Dudek T. Wierzchoń Jarosław Paluch

Using NiTi alloys with shape memory for long-term medical implants requires modification of their surface due to the possible occurrence of corrosion. Hence, the surface of the staples used to join fractured bone within the craniofacial region was modified by applying a titanium oxy-nitrogen layer and a hydroxyapatite coating. Surface-modified clamps were tested in vivo using New Zealand white rabbits. After determining the mechanical characteristics of the bone and considering the initial state and surface modification, the diameter of the wire (used to make the clamps with the appropriate compression force) was selected. Implantation was performed on two groups of rabbits: experimental and control. In the experimental group, an intentionally induced bone fracture was treated in one tibia. On the second tibia, two additional clamps were applied to increase the possibility of a negative impact of the NiTi alloy on a living organism. After 6 weeks of application, a proper joining of the broken bone fragments was stated. Whereas after twelve weeks, no negative impact of the clamp material on a living organism, i.e., a rabbit, was found. Hence, the clamp with the modified surface can connect bone fragments in humans as well as small and medium-sized animals, with an extended range of use up to 12 weeks.

Nickel titanium

Clamp

Titanium alloy

Bone fracture

10.3390/ma16165575

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Citations (4)

Ultrastructural investigations of Haller’s organ in Dermacentor reticulatus (Fabr.) (Acari: Ixodida: Ixodidae)

Springer eBooks (2002)

Alicja Buczek L Buczek A. Kusmierz Krzysztof Olszewski Krzysztof Jasik

Dermacentor reticulatus

Dermacentor

10.1007/978-94-017-0611-7_23

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Citations (3)

The epidemiological situation of chickenpox in the Silesian voivodeship in the years 2011-2015 in view of data of the situation in Poland

PubMed (2017)

Renata Cieślik-Tarkota Zbigniew Lorenc Marta Albertyńska Beata Rozwadowska Krzysztof Jasik

Despite the availability of preventive vaccination against chickenpox, this form of prevention is rarely used and is not available to the entire population of children. In order to evaluate an acquired immunity against the virus Varicella-Zoster examining of the presence of specific IgG antibodies to VZV in serum or plasma is required. The aim of this study was to assess the epidemiological situation of chickenpox in Śląskie Voivodeship in 2011-2015.The evaluation of the epidemiological situation of chickenpox in the past five years was based on analysis of data from the bulletin “Infectious diseases and poisonings in Poland” for the period 2011-2014 and 2015 data, received from the Provincial Sanitary and Epidemiological Stations. Analysis of the vaccinated population was made on the basis of data available in the bulletin “Vaccinations in Poland” for the period 2011-2014 and 2015 data obtained from the NIPH-PZH. Samples of patients from Śląskie were tested with the use of Novalisa Varicella-Zoster Virus (VZV) IgG - ELISA (Novatec Immunodiagnostic GMBH, Germany). Samples were delivered to the Laboratory of the Provincial Sanitary and Epidemiological Station in the course of 2011-2015.Between 2011 and 2015, in Śląskie, 136 094 chickenpox cases were registered (14% of all occurring in Poland). Based on the number of cases, Śląskie is ranked second place, just after Mazowieckie, in which during the same period of time 143 392 illness were registered. The average annual incidence in Śląskie was 591 per 100 thousand residents. Between 2011 and 2015, a total of 360 serum samples were examined. The percentage of positive IgG in each year ranged between 59.6 and 75.7%.Promoting vaccination and preventing the sick children to contact the healthy ones as well as the protection of adults susceptible to infection can improve the epidemiological situation regarding incidences of chickenpox. Thanks to vaccines the risk of incidence of chickenpox can be reduced or even the incidences can be prevented. Information about acquired immunity, acquired before the pregnancy, allows to take the action in order to protect the mother from getting chickenpox in form of a preventive vaccination.

Chicken Pox

Chickenpox Vaccine

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Comparative Study on Human Cartilage in Laryngological Transplantation Surgery Purposes by Means Confocal Fluorescence Microscope – Primary Study

IFMBE proceedings (2014)

Wojciech Ciszek Z. Drzazga Jarosław Paluch Krzysztof Jasik Grzegorz Bajor

Meniscus

10.1007/978-3-319-11128-5_43

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First attempts to 3D printing spine implants : human spine biomechanics

M. Gaweł A. Guia Krzysztof Jasik Beata Swinarew Hubert Okła

Biomechanics

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Origin of Alimentary Tract in Embryogenesis of Ixodes ricinus (Acari: Ixodidae)

Journal of Medical Entomology (2005)

Krzysztof Jasik Alicja Buczek

We studied the embryos of Ixodes ricinus (L.) in the second and third trimester of embryonic development, by using light and transmission electron microscopy. At the beginning of the second trimester, the formation of the foregut and rectal sac, by a process of invagination, was observed. The invagination, which develops into the primordium of the hindgut, forms only in the third trimester. The rectum forms in the last phase of embryogenesis. The development of the midgut is incomplete during embryogenesis. The yolk is surrounded by a wall, formed of an amorphous basal lamina and flattened cells, that gradually accumulate deutoplasmic material. These cells do not acquire the typical features of the gut epithelium until after larval hatching. These features are, however, found in the cells forming the rectal sac.

Hindgut

Foregut

Primordium

Embryology

10.1603/0022-2585(2005)042[0541:ooatie]2.0.co;2

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Citations (5)

Hepatic Tissue Changes in Rats Due to Chronic Invasion of <I>Babesia microti</I>

Folia Biologica (2014)

Hubert Okła Krzysztof Jasik Jan Słodki Beata Rozwadowska Aleksandra Słodki

The etiological agents of babesiosis are intraerythrocytic parasites of the genus Babesia, which are transmitted by ticks. The course of disease is characterized by variable severity. The risk of a complicated course of babesiosis occurs in premature infants, the elderly, splenectomized patients and other immunocompromised patients. Severe cases of this disease can lead to multiple organ dysfunction. The study focuses on the impact assessment of chronic Babesia microti invasion on the morphology and ultrastructure of rat liver. The analyzed material was comprised of liver samples collected from Wistar rats infected with a reference strain of B. microti (ATCC 30221). None of the livers collected from rats with babesiosis was enlarged. The histopathological analyses showed signs of intensive inflammatory processes, especially in the perivascular areas. The hepatic mononuclear phagocyte system was characterized by increased activity. The ultrastructral analyses confirmed disintegration of hepatocytes with vacuolization in the perivascular areas. In addition, the perisinusoidal space (space of Disse) had irregular structure. In some areas, the space of Disse was enlarged or compressed. The morphological and ultrastructural analyses of rat liver with chronic babesiosis caused by B. microti showed significant pathological changes in perivascular areas which may be the cause of hepatic dysfunction.

Perisinusoidal space

Histopathology

Vacuolization

10.3409/fb62_4.353

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Citations (11)