Katarína Kozics

Slovak Academy of Sciences

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Alena Gábelová

Cancer Research Institute of the Slovak Academy of Sciences

Eva Horváthová

Faculty of Public Health

Darina Slameňová

Slovak Academy of Sciences

MŠ

Monika Šrámková

Slovak Academy of Sciences

Annamária Srančíková

Slovak Academy of Sciences

Martina Melušová

Comenius University Bratislava

Mária Bučková

Institute of Molecular Biology of the Slovak Academy of Sciences

Víctor Puntes

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

PŠ

Peter Šimon

Slovak University of Technology in Bratislava

Tibor Dubaj

Slovak University of Technology in Bratislava

Cooperative Institutions

Slovak Academy of Sciences

101

Comenius University Bratislava

Cancer Research Institute of the Slovak Academy of Sciences

Biomedical Research Center of the Slovak Academy of Sciences

Slovak Medical University

Czech Academy of Sciences

Institute of Chemistry of the Slovak Academy of Sciences

Slovak University of Technology in Bratislava

Charles University

Cancer Institute (WIA)

Author Statistics

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Rapid identification of in vitro cell toxicity using an electrochemical membrane screening platform

Bioelectrochemistry (2023)

Yvonne Kohl Nicola William Elisabeth Elje Nadine Backes Mario Rothbauer

This study compares the performance and output of an electrochemical phospholipid membrane platform against respective in vitro cell-based toxicity testing methods using three toxicants of different biological action (chlorpromazine (CPZ), colchicine (COL) and methyl methanesulphonate (MMS)). Human cell lines from seven different tissues (lung, liver, kidney, placenta, intestine, immune system) were used to validate this physicochemical testing system. For the cell-based systems, the effective concentration at 50 % cell death (EC50) values are calculated. For the membrane sensor, a limit of detection (LoD) value was extracted as a quantitative parameter describing the minimum concentration of toxicant which significantly affects the structure of the phospholipid sensor membrane layer. LoD values were found to align well with the EC50 values when acute cell viability was used as an end-point and showed a similar toxicity ranking of the tested toxicants. Using the colony forming efficiency (CFE) or DNA damage as end-point, a different order of toxicity ranking was observed. The results of this study showed that the electrochemical membrane sensor generates a parameter relating to biomembrane damage, which is the predominant factor in decreasing cell viability when in vitro models are acutely exposed to toxicants. These results lead the way to using electrochemical membrane-based sensors for rapid relevant preliminary toxicity screens.

Toxicant

Viability assay

EC50

Cell membrane

10.1016/j.bioelechem.2023.108467

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Citations (9)

Assessment of Antioxidative, Chelating, and DNA-Protective Effects of Selected Essential Oil Components (Eugenol, Carvacrol, Thymol, Borneol, Eucalyptol) of Plants and Intact Rosmarinus officinalis Oil

Journal of Agricultural and Food Chemistry (2014)

Eva Horváthová Jana Navarová Eliška Gálová Andrea Ševčovičová Lenka Chodáková

Selected components of plant essential oils and intact Rosmarinus officinalis oil (RO) were investigated for their antioxidant, iron-chelating, and DNA-protective effects. Antioxidant activities were assessed using four different techniques. DNA-protective effects on human hepatoma HepG2 cells and plasmid DNA were evaluated with the help of the comet assay and the DNA topology test, respectively. It was observed that whereas eugenol, carvacrol, and thymol showed high antioxidative effectiveness in all assays used, RO manifested only antiradical effect and borneol and eucalyptol did not express antioxidant activity at all. DNA-protective ability against hydrogen peroxide (H2O2)-induced DNA lesions was manifested by two antioxidants (carvacrol and thymol) and two compounds that do not show antioxidant effects (RO and borneol). Borneol was able to preserve not only DNA of HepG2 cells but also plasmid DNA against Fe2+-induced damage. This paper evaluates the results in the light of experiences of other scientists.

Thymol

Borneol

Carvacrol

Rosmarinus

Camphor

Eucalyptol

10.1021/jf501006y

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Citations (105)

Antioxidant activity of resveratrol, piceatannol and 3,3',4,4',5,5'-hexahydroxy-trans-stilbene in three leukemia cell lines

Oncology Reports (2006)

Z Ovesná Katarína Kozics Yvonne Bader Philipp Saiko Norbert Handler

Piceatannol

K562 cells

10.3892/or.16.3.617

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Citations (94)

The antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human HEL 12469 cells

Scientific Reports (2017)

Andrea Puškárová Mária Bučková Lucia Kraková Domenico Pangallo Katarína Kozics

Abstract Six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. Antimicrobial activity was shown against pathogenic ( Escherichia coli , Salmonella typhimurium , Yersinia enterocolitica , Staphylococcus aureus , Listeria monocytogenes , and Enterococcus faecalis ) and environmental bacteria ( Bacillus cereus , Arthrobacter protophormiae , Pseudomonas fragi ) and fungi ( Chaetomium globosum, Penicillium chrysogenum , Cladosporium cladosporoides , Alternaria alternata , and Aspergillus fumigatus) . Oregano, thyme, clove and arborvitae showed very strong antibacterial activity against all tested strains at both full strength and reduced concentrations. These essential oils showed different fungistatic and fungicidal activities when tested by direct application and in the vapor phase. The genotoxic effects of these oils on HEL 12469 human embryo lung cells were evaluated using an alkaline comet assay for the first time, revealing that none of the oils induced significant DNA damage in vitro after 24 h. This study provides novel approaches for assessing the antimicrobial potential of essential oils in both direct contact and the vapor phase and also demonstrates the valuable properties of the phenol-free arborvitae oil. These results suggest that all the tested essential oils might be used as broad-spectrum anti-microbial agents for decontaminating an indoor environment.

Comet Assay

10.1038/s41598-017-08673-9

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Citations (207)

Pharmacokinetics of PEGylated Gold Nanoparticles: In Vitro—In Vivo Correlation

Nanomaterials (2022)

Tibor Dubaj Katarína Kozics Monika Šrámková Alena Manová Neus G. Bastús

Data suitable for assembling a physiologically-based pharmacokinetic (PBPK) model for nanoparticles (NPs) remain relatively scarce. Therefore, there is a trend in extrapolating the results of in vitro and in silico studies to in vivo nanoparticle hazard and risk assessment. To evaluate the reliability of such approach, a pharmacokinetic study was performed using the same polyethylene glycol-coated gold nanoparticles (PEG-AuNPs) in vitro and in vivo. As in vitro models, human cell lines TH1, A549, Hep G2, and 16HBE were employed. The in vivo PEG-AuNP biodistribution was assessed in rats. The internalization and exclusion of PEG-AuNPs in vitro were modeled as first-order rate processes with the partition coefficient describing the equilibrium distribution. The pharmacokinetic parameters were obtained by fitting the model to the in vitro data and subsequently used for PBPK simulation in vivo. Notable differences were observed in the internalized amount of Au in individual cell lines compared to the corresponding tissues in vivo, with the highest found for renal TH1 cells and kidneys. The main reason for these discrepancies is the absence of natural barriers in the in vitro conditions. Therefore, caution should be exercised when extrapolating in vitro data to predict the in vivo NP burden and response to exposure.

Biodistribution

10.3390/nano12030511

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Citations (27)

Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells

Molecules (2017)

Petronela Imreová Jana Feruszová Stanislav Kyzek Kristína Bodnárová Martina Zdurienčíková

Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John’s wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity of HF has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. The present work is designed to investigate the potential antigenotoxic effects of HF using three different experimental test systems. The antigenotoxic effect of HF leading to the decrease of primary/transient promutagenic genetic changes was detected by the alkaline comet assay on human lymphocytes. The HF antimutagenic effect leading to the reduction of gene mutations was assessed using the Ames test on the standard Salmonella typhimurium (TA97, TA98, and TA100) bacterial strains, and the anticlastogenic effect of HF leading to the reduction of chromosome aberrations was evaluated by the in vitro mammalian chromosome aberration test on the human tumor cell line HepG2 and the non-carcinogenic cell line VH10. Our findings provided evidence that HF showed antigenotoxic effects towards oxidative mutagen zeocin in the comet assay and diagnostic mutagen (4-nitroquinoline-1-oxide) in the Ames test. Moreover, HF exhibited an anticlastogenic effect towards benzo(a)pyrene and cisplatin in the chromosome aberration test.

Ames test

Chromosome aberration

Comet Assay

Clastogen

Phloroglucinol

Hyperforin

10.3390/molecules22010167

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Citations (16)

Protective effects of ursolic acid and oleanolic acid in leukemic cells

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis (2006)

Z Ovesná Katarína Kozics Darina Slameňová

Ursolic acid

10.1016/j.mrfmmm.2006.03.008

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Citations (119)

Electrochemical behavior and determination of tumor inhibiting or promoting activities of flavonoids.

Neoplasma (2006)

Katarína Kozics Zdenka Krajčovičová A Vachálková

This paper deals with determination of the tumor inhibiting or promoting activities of 11 flavonoids performed by DC polarography. Flavonoids· were tested in the presence of polyaromatic carcinogen 7,12-dimethylbenz(a)anthracene (DMBA) or in combination with DMBA and 12-O-tetradecanoylphorbol-13-acetate (TPA), which is known as a specific tumor promoter for epidermal carcinogenesis. We found that in this experimental system the promotory or inhibitory activities of studied flavonoids depend on the number and position of hydroxyl groups in their chemical structures and are related to the polarographic behavior of these compounds. Flavonoids, which are hydroxylated at the ring B, are reduced in anhydrous DMF on a mercury dropping electrode in two one-electron steps. Absence of the hydroxyl groups in these positions caused their reduction in three one-electron steps. Similarly, flavonoids with hydroxyl groups at the ring B have been shown to inhibit the activities of DMBA (2.7-45.9%) and of DMBA + TPA (47.2-78.2%). Missing hydroxyl groups caused weaker inhibitory activity against DMBA + TPA (19.01-38.74%) and the enhancement of DMBA activity (31.08-66.21%). Presented data demonstrated that the electrochemical method - DC polarography is very sensitive, simple technique for determination of the tumor inhibiting or promoting activities of the studied compounds.

Polarography

Source

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Citations (1)

Kidney nanotoxicity studied in human renal proximal tubule epithelial cell line TH1

Mutation Research/Genetic Toxicology and Environmental Mutagenesis (2019)

Monika Šrámková Katarína Kozics Vlasta Mašánová Iveta Uhnáková Filip Rázga

Progressive expansion of nanomaterials in our everyday life raises concerns about their safety for human health. Although kidneys are the primary organs of xenobiotic elimination, little attention has been paid to the kidneys in terms of nanotoxicological studies up to now. Here we investigate the cytotoxic and genotoxic potential of four solid-core uncoated inorganic nanoparticles (TiO2NPs, SiO2NPs, Fe3O4NPs and AuNPs) using the human renal proximal tubule epithelial TH1 cells. To mimic the in vivo conditions more realistic, TH1 cells were exposed in vitro to inorganic NPs under static as well as dynamic conditions for 3 h and 24 h. The medium throughput alkaline comet assay (12 minigels per slide) was employed to evaluate the impact of these NPs on genome integrity and their capacity to produce oxidative lesions to DNA. The accumulation and localization of studied inorganic NPs inside the cells was monitored by transmission electron microscopy (TEM) and the efficacy of internalization of particular NPs was determined by atomic absorption spectroscopy (AAS) and inductively coupled plasma mass spectrometry (ICP-MS). From all the tested NPs, only Fe3O4NPs induced a slight cytotoxicity in TH1 cells exposed to high concentrations (>700 μg/ml) for 24 h. On the other hand, the inorganic NPs did not increase significantly the level of DNA strand breaks or oxidative DNA damage regardless of the treatment mode (static vs. dynamic conditions). Interestingly, substantial differences were observed in the internalized amount of inorganic NPs in TH1 cells exposed to equivalent (2.2 μg/ml) concentration. Fe3O4NPs were most efficiently taken up while the lowest quantity of particles was determined in TiO2NPs-treated cells. As the particle size and shape of individual inorganic NPs in culture medium was nearly identical, it is reasonable to suppose that the chemical composition may contribute to the differences in the efficacy of NPs uptake.

Nanotoxicology

Comet Assay

Internalization

Nanomaterials

10.1016/j.mrgentox.2019.01.012

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Citations (25)

Electrospun Poly(ethylene Terephthalate)/Silk Fibroin Composite for Filtration Application

Polymers (2021)

Alena Opálková Šišková Katarína Mosnáčková Jakub Hrůza Jaroslava Frajová Andrej Opálek

In this study, fibrous membranes from recycled-poly(ethylene terephthalate)/silk fibroin (r-PSF) were prepared by electrospinning for filtration applications. The effect of silk fibroin on morphology, fibers diameters, pores size, wettability, chemical structure, thermo-mechanical properties, filtration efficiency, filtration performance, and comfort properties such as air and water vapor permeability was investigated. The filtration efficiency (FE) and quality factor (Qf), which represents filtration performance, were calculated from penetration through the membranes using aerosol particles ranging from 120 nm to 2.46 μm. The fiber diameter influenced both FE and Qf. However, the basis weight of the membranes has an effect, especially on the FE. The prepared membranes were classified according to EN149, and the most effective was assigned to the class FFP1 and according to EN1822 to the class H13. The impact of silk fibroin on the air permeability was assessed. Furthermore, the antibacterial activity against bacteria S. aureus and E. coli and biocompatibility were evaluated. It is discussed that antibacterial activity depends not only on the type of used materials but also on fibrous membranes’ surface wettability. In vitro biocompatibility of the selected samples was studied, and it was proven to be of the non-cytotoxic effect of the keratinocytes (HaCaT) after 48 h of incubation.

Fibroin

Biocompatibility

Filtration (mathematics)

Electrospinning

10.3390/polym13152499

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Citations (34)