Pierre Chaminade

Université Paris-Saclay

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A. Baillet

Université Paris-Sud

Karen Gaudin

Université de Bordeaux

Sonia Abreu

Université Paris-Saclay

Danielle Libong

Inserm

P. Prognon

Assistance Publique – Hôpitaux de Paris

Audrey Solgadi

Inserm

D. Ferrier

Université Paris-Saclay

S. Roy

Hôpital Antoine-Béclère

Dominique P. Germain

Assistance Publique – Hôpitaux de Paris

Olivier Laprévôte

Centre National de la Recherche Scientifique

Cooperative Institutions

Centre National de la Recherche Scientifique

187

Université Paris-Saclay

150

Université Paris-Sud

146

Institut Galien Paris-Saclay

Université Paris Cité

Inserm

Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion

Assistance Publique – Hôpitaux de Paris

Sorbonne Université

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

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Rapid analysis of glutamate, glutamine and GABA in mice frontal cortex microdialysis samples using HPLC coupled to electrospray tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis (2018)

Céline Defaix Audrey Solgadi Thu Ha Pham Alain M. Gardier Pierre Chaminade

Microdialysis

Glutamic acid

10.1016/j.jpba.2018.01.039

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Citations (43)

QUANTITATIVE STUDY OF THE STRATUM CORNEUM LIPID CLASSES BY NORMAL PHASE LIQUID CHROMATOGRAPHY: COMPARISON BETWEEN TWO UNIVERSAL DETECTORS

Journal of Liquid Chromatography &amp Related Technologies (2010)

Chloé Merle Cécile Laugel Pierre Chaminade Arlette Baillet‐Guffroy

The stratum corneum owns a barrier function, which can be altered by a modification of its lipid composition. In this work, a new simple method was developed in normal phase liquid chromatography to separate the three major lipid classes of the stratum Corneum, fatty acids, ceramides, and cholesterol. A quantitative investigation was carried out, comparing an internal normalisation and an external standardisation with two universal detectors: an evaporating light scattering detector and a charged aerosol detector, the Corona. The study points out the fact that the external standardisation is suitable for a quantitative study of the different lipid classes, using a representative standard for each class. Moreover, the use of the Corona is more adapted for further investigation, due to a better repeatability, accuracy, and precision. A test made on a lipid extract from the forearm of volunteers shows that the present method is appropriate for biological extract analysis.

Repeatability

Quantitative Analysis

10.1080/10826071003608520

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Citations (15)

Atmospheric Pressure Photoionization as a Powerful Tool for Large-Scale Lipidomic Studies

Journal of the American Society for Mass Spectrometry (2012)

Mathieu Gaudin Laurent Imbert Danielle Libong Pierre Chaminade Alain Brunelle

Lipidomic studies often use liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) for separation, identification, and quantification. However, due to the wide structural diversity of lipids, the most apolar part of the lipidome is often detected with low sensitivity in ESI. Atmospheric pressure (APPI) can be an alternative ionization source since normal-phase solvents are known to enhance photoionization of these classes. In this paper, we intend to show the efficiency of APPI to identify different lipid classes, with a special interest on sphingolipids. In-source APPI fragmentation appears to be an added value for the structural analysis of lipids. It provides a detailed characterization of both the polar head and the non polar moiety of most lipid classes, and it makes possible the detection of all lipids in both polarities, which is not always possible with ESI.

Lipidome

Fragmentation

Atmospheric-pressure chemical ionization

Cardiolipins

10.1007/s13361-012-0341-y

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Citations (26)

Eicosapentaenoic acid membrane incorporation impairs cholesterol efflux from cholesterol-loaded human macrophages by reducing the cholesteryl ester mobilization from lipid droplets Molecular and cell biology of lipids

Biochimica et Biophysica Acta (2017)

Natalie Fournier Guillaume Sayet Benoît Védie Maxime Nowak Fatima Allaoui

Docosapentaenoic acid

Cholesteryl ester