Raluca M. Fratila

Instituto de Nanociencia y Materiales de Aragón

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Jesús M. de la Fuente

Universidad de Zaragoza

J. M. Aizpurua

University of the Basque Country

Valeria Grazú

Universidad de Zaragoza

José I. Miranda

University of the Basque Country

Lucía Gutiérrez

Instituto de Nanociencia y Materiales de Aragón

Aldrik H. Velders

Wageningen University & Research

Laura Asín

Universidad de Zaragoza

Lilianne Beola

Piaggio (Italy)

Claudio Palomo

University of the Basque Country

Francisco Javier Idiago López

Cooperative Institutions

University of the Basque Country

Universidad de Zaragoza

Instituto de Nanociencia y Materiales de Aragón

University of Twente

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Consejo Superior de Investigaciones Científicas

Centre National de la Recherche Scientifique

University of Bonn

University of Zurich

Centro de Investigación Biomédica en Red

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Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity

Nano Biomedicine and Engineering (2018)

Paula Sanguiñedo Raluca M. Fratila María Belén Estevez Jesús M. de la Fuente Valeria Grazú

Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential.The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided.The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis.One of the main applications of silver nanoparticles is their antimicrobial activity.Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state.In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied.Eight fungi were able to synthesize nanoparticles.An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility.According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays.All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications.

Extracellular polymeric substance

Silver nanoparticle

10.5101/nbe.v10i2.p165-173

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Citations (31)

Lymphatic mapping and sentinel lymph node biopsy in an in-vivo porcine model using a novel magnetic technique

European Journal of Cancer (2013)

Bauke Anninga Munir Ahmed Joost Pouw Raluca M. Fratila B. ten Haken

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Radiation free localization and diagnosis of sentinel lymph nodes with magnetic nano-particles

Raluca M. Fratila Joost Pouw Aldrik H. Velders B. ten Haken

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Bioorthogonal click chemistry meets nanotechnology: covalent immobilization of magnetic nanoparticles on cell membranes

Francisco Javier Idiago López Eduardo Moreno Antolín Raluca M. Fratila Jesús M. de la Fuente

Resumen del trabajo presentado a la IV Reunion de Jovenes Investigadores en Coloides e Interfases, celebrada en Cordoba del 7 al 9 de febrero de 2018.

Bioorthogonal Chemistry

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Cyclic RGD β‐Lactam Peptidomimetics Induce Differential Gene Expression in Human Endothelial Cells

ChemBioChem (2011)

J. M. Aizpurua José Ignacio Ganboa Claudio Palomo Iraida Loinaz Joseba Oyarbide

RGD peptidomimetics touch DNA: A few RGD cyclic peptidomimetics, each incorporating a densely substituted β-lactam moiety, were prepared, and their effect on extracellular adhesion and intracellular gene regulation through αVβ3 integrin was studied in human endothelial cells (HUVECs). Surprisingly, the cyclic tetrapeptide with a deleted glycine residue and its parent RGD peptidomimetic displayed practically opposite angiogenic gene-regulation activity.

Peptidomimetic

Tetrapeptide

RGD motif

Moiety

10.1002/cbic.201000572

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Química click bioortogonal e hipertermia magnética: una nueva estrategia para la transfección celular

Francisco Javier Idiago López Eduardo Moreno Antolín Jesús M. de la Fuente Valeria Grazú Raluca M. Fratila

Resumen del poster presentado a la 8a Jornada de Jovenes Investigadores (Quimica y Fisica) de Aragon, celebrada en Zaragoza el dia 22 de noviembre de 2018.

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Mechanistic Insights on the Magnesium(II) Ion-Activated Reduction of Methyl Benzoylformate with Chelated NADH Peptide β-Lactam Models

The Journal of Organic Chemistry (2009)

J. M. Aizpurua Claudio Palomo Raluca M. Fratila Pablo Ferrón Ana Benito

Mechanistic details of the Mg2+ ion-activated enantioselective reduction of methyl benzoylformate have been investigated at a B3LYP/6-31G* theory level, using peptide NADH models 1 rigidified with a β-lactam ring. Computation of the reaction pathway revealed important structural differences between the intermediate NADH/Mg2+/ArCOCO2R ternary complexes 3 and the corresponding transition states leading to enantiomeric methyl mandelates. Thus, ternary complexes showed the dihydronicotinamide moiety placed quasiequatorial to a seven-membered chelation pseudoplane including the two amide carbonyls and the Mg2+ cation, whereas productive transition states were strongly deformed with the dihydronicotinamide group oriented quasiaxial to the chelation pseudoplane. This chelation model was further applied to acyclic nonrigidified NADH models and, based on the fluxional mobility of the peptide chain bonds, experimental enantioselectivities were correctly predicted. Parallel experiments were also conducted in deuterated acetonitrile, using NMR techniques, to study the structure of the binary complexes 2 (NADH/Mg2+) and ternary complexes 3 (NADH/Mg2+/PhCOCO2Me). Finally, owing to the incorporation of two diastereotopic trimethylsilyl NMR-tags in the β-lactam-NADH peptidomimetics, a nonproductive ternary complex predicted by calculations could be observed and its structure characterized on the basis of ROESY experiments and molecular modeling.

Lactam

10.1021/jo901236d

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Quantification of Lipoprotein Uptake in Vivo Using Magnetic Particle Imaging and Spectroscopy

ACS Nano (2020)

Staffan Hildebrand Norbert Löwa Hendrik Paysen Raluca M. Fratila Laia Reverte-Salisa

Lipids are a major source of energy for most tissues, and lipid uptake and storage is therefore crucial for energy homeostasis. So far, quantification of lipid uptake in vivo has primarily relied on radioactive isotope labeling, exposing human subjects or experimental animals to ionizing radiation. Here, we describe the quantification of in vivo uptake of chylomicrons, the primary carriers of dietary lipids, in metabolically active tissues using magnetic particle imaging (MPI) and magnetic particle spectroscopy (MPS). We show that loading artificial chylomicrons (ACM) with iron oxide nanoparticles (IONPs) enables rapid and highly sensitive post hoc detection of lipid uptake in situ using MPS. Importantly, by utilizing highly magnetic Zn-doped iron oxide nanoparticles (ZnMNPs), we generated ACM with MPI tracer properties superseding the current gold-standard, Resovist, enabling quantification of lipid uptake from whole-animal scans. We focused on brown adipose tissue (BAT), which dissipates heat and can consume a large part of nutrient lipids, as a model for tightly regulated and inducible lipid uptake. High BAT activity in humans correlates with leanness and improved cardiometabolic health. However, the lack of nonradioactive imaging techniques is an important hurdle for the development of BAT-centered therapies for metabolic diseases such as obesity and type 2 diabetes. Comparison of MPI measurements with iron quantification by inductively coupled plasma mass spectrometry revealed that MPI rivals the performance of this highly sensitive technique. Our results represent radioactivity-free quantification of lipid uptake in metabolically active tissues such as BAT.

Chylomicron

Lipoprotein particle

Iron oxide nanoparticles

10.1021/acsnano.0c03229

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Membrane-localized magnetic hyperthermia promotes intracellular delivery of cell-impermeant probes

Nanoscale (2024)

Javier Idiago-López Daniela Ferreira Laura Asín María Moros Ilaria Armenia

In this work, we report the disruptive use of membrane-localized magnetic hyperthermia to promote the internalization of cell-impermeant probes. Under an alternating magnetic field, magnetic nanoparticles (MNPs) immobilized on the cell membrane

Cell membrane

10.1039/d4nr01955e

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Plasma Polymerized Silylated Ciprofloxacin as an Antibiotic Coating

Plasma Processes and Polymers (2011)

I. Braceras Patxi Azpiroz Nerea Briz Raluca M. Fratila Joseba Oyarbide

Abstract Locally applied antibiotics under temporally controlled release present many advantages over systemic clinical treatments, e.g. efficiency and side effects. This can be achieved by a coating on top of the medical device, in which the antibiotic is stored. This study presents the use of plasma polymerization to produce such a coating using N,O‐bis‐tert‐butyldimethylsilylated ciprofloxacin (silylciprofloxacin) as a precursor. Once exposed to physiological media, the outer layers of the coating release the antibiotic by a hydrolysis reaction. Thus, the plasma process parameters can control the speed of liberation through the coating polymerization. Besides, this study shows that the release products present antibiotic activity against a number of bacteria: E. coli, P. aeruginosa , and S. aureus . magnified image

Plasma polymerization

10.1002/ppap.201000160

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Citations (11)