Ren-Gao Xue

Qingdao Agricultural University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Hong-Feng Xie

Qingdao Agricultural University

Biao Zhang

Northwestern Polytechnical University

Chunmei Zhao

Qingdao Agricultural University

Mengge Xing

Qingdao Agricultural University

Heng Hou

First Hospital of Shanxi Medical University

Yingying Ma

Institute of Oceanology

Biao Zhang

Guilin Medical University

Biao Zhang

Huazhong University of Science and Technology

Zhu BaoGe

Chinese Academy of Sciences

Cao Yinsheng

Cooperative Institutions

Chinese Academy of Sciences

Qingdao Agricultural University

University of Chinese Academy of Sciences

Xinjiang University

Hunan University

Zhejiang University

Huazhong University of Science and Technology

Guangxi University

Institute of Genetics and Developmental Biology

South China Agricultural University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Study on the Factors Affecting the Genetic Transformation Efficiency of Soybean Germinating Seeds Mediated by Agrobacterium tumefacines

Anhui nongye kexue (2008)

Ren-Gao Xue

[Objective] The aim of the research was to enhance the genetic transformation efficiency of soybean mediated by Agrobacterium tumefacines.[Method] With half piece of soybean seeds germinated for 1 d as target tissue,the effects of acetosyringone and A.tumefacines liquid concn.and infection time on the transformation efficiency were studied through GUS transient expression detection.[Result] When 200 μmol/L acetosyringone was added to the co-culture medium,GUS transient expression efficiency was highest.When A. tumefacines grew up to logarithmic growth stage(D600 nm of 0.5),the transformation efficiency was highest and 9.4 blue spots averagely appeared in each explant.Too long infection time was not suitable,with 30～60 min being better.[Conclusion] The genetic transformation efficiency of soybean mediated by A.tumefacines was affected by acetosyringone concn.,the liquid concn.of A.tumefacines and the infection time

Acetosyringone

Transformation efficiency

Source

Cite

Citations (0)

Physiological and biochemical characteristics of adventitious bud formation in soybean mature-cotyledon (in vitro culture

Zuo wu xue bao (1995)

Liu Shulan Ren-Gao Xue Wu Zhengdao Han Biwen Zhenghua Chen

Maturecotyledon explants of soybean were cultured on MS medium with 2.0mg/L BA. The adventitious buds were differentiated after 10 days of culture and rootless plantlet were obtained after 30 days of culture. It was shown that by SDS-PAGE analysis, the bands of four proteins with molecular weights of 19KD, 36kD, 48kD and 72kD respectively disappeared at the fifth day of culture, the new bands of proteins with molecular weights of 25kD and 27kD appeared at the fourth day of culture, and those of proteins with 32kD and 33.8kD appeared at the fifth day of culture. The content of protein with molecular weight of 55kD increased obviously after 5 days of culture and the protein with molecular weight of 82kD and those with molecular weights below 16kD were stable during the culture.

Cotyledon

Plantlet

Molecular mass

Source

Cite

Citations (0)

Induction of multiple shoots from stem explants of Chardonny grape.

Guoshu xuebao (2009)

Deng Jie Xin Liu Ren-Gao Xue

In order to induce multiple shoots from Chardonny grape(Vitis vinifera)explants,the effects of explants,media,hormone concentrations and dark treatment on the induction of multiple shoots were studied.The shoots were induced only from the stem explants,the induction frequency reached to 17.5%.There was no difference in the average number of shoots a-mong 3 media of MS,NN1969 and B5.The shoots were induced from the stems on the MS medium supplemented with BA and IBA,and there was significant difference in the frequency of shoot induction among different concentration treatments of BA and IBA.However,there was no significant difference in the average number of shoots and most of shoots induced were single shoot,indicated that there was no significant effect of the combination of BA with IBA on the induction of multiple shoots in this study.The significant effect of the combination of BA with NAA on both the induction frequency of shoots and the number of multiple shoots was observed.The highest induction frequency(9.7%)of shoots and the highest average number of shoots(10.1)were induced from the stems on the MS medium supplemented with 3.0 mg·L-1 BA and 0.1 mg·L-1 NAA,the highest number from one stem reached up to 27.The roots were induced from the shoots on the 1/2MS medium supplemented with 0.05 mg·L-1 IAA.

Source

Cite

Citations (3)

Optimizing conditions for Agrobacterium-mediated transformation of Chardonnay grape cultivar's embryogenic cells

Guoshu xuebao (2008)

Ren-Gao Xue

To establish an efficient transformation of grape(Vitis vinifera),the factors affecting the Agrobacterium-mediated transformation efficiency were studied by GUS assay using the embryogenic cells as the target tissue.The embryogenic cells were sonicated and inoculated with Agrobacterium EHA105/pCAMBIA1301,and placed on co-culture medium containing different concentrations of acetosyringone and dithiothreitol.The transient expression of the gene gus was assayed at 3 or 4 d after co-cultivation for transformation.The highest transformation efficiency was obtained when the embryogenic cells were sonicated for 5 min.The concentration of acetosyringone also affected the transformation efficiency.The average blue spots were determined to be 8.89 at 50 μmol/L AS,and 12.44 at 100 μmol/L AS.The addition of DTT to the co-culture medium also enhanced transient GUS expression.The blue spots were reached up to 16.67 at 3 mg/L of DTT.In this study,the optimum factors affecting the Agrobacterium-mediated transformation of grape embryogenic cells were established by GUS transient expression assay.These results will provide useful information for establishment of an efficient transformation method of grape.

Acetosyringone

Transformation efficiency

Source

Cite

Citations (0)

Cloning and Expression Analysis of <i>GmCYP78A5</i> Promoter

American Journal of Plant Biology (2019)

Xiaofeng Chen Qiuli Du Chun‐Mei Zhao Zhaoyong Lv Ren-Gao Xue

CYP78A5 promoter was isolated from soybean (Glycine max L. Merrill) plant by using PCR technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was 99.21% homologous to the correspondent regions of the reported sequences. Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression elements. RT-PCR results indicated that the gene GmCYP78A5 highly expressed in immature seed, weekly expressed in stem of soybean, but no expressed in root, leaf and flower. To further study the tissue expression patterns of GmCYP78A5 gene, the promoter of the gene GmCYP78A5 was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into tobacco (Nicotiana tabacum) by Agrobacterium-meditated method. The expression of the GUS gene in the transgenic tobacco plants indicated that the GmCYP78A5 promoter could drive the GUS reporter gene to express highly in the leaf, stem, sepal, pedicel, seeds of the transgenic tobacco plants, demonstrating that the expression patterns of the GmCYP78A5 promoters in soybean and tobacco were inconsistent.

GUS reporter system

Beta-glucuronidase

Cloning (programming)

Pedicel

10.11648/j.ajpb.20190401.12

Cite

Citations (0)

Overexpression of a NTR1 in transgenic soybean confers tolerance to water stress

Plant Cell Tissue and Organ Culture (PCTOC) (2007)

Ren-Gao Xue Biao Zhang Hong-Feng Xie

Methyl jasmonate

Jasmonic acid

Drought Tolerance

10.1007/s11240-007-9231-6

Cite

Citations (21)

High Frequency Induction of Callus from Soybean Cotyledonary Node Explants and Its Plant Regeneration

Dadou kexue (2011)

Ren-Gao Xue

Effects of germination medium and different concentration ratios of BA and TDZ on the callus induction and plant regeneration were studied using fragments and slices of 7 day-germinated soybean cotyledonary nodes as explants.The result showed that the callus formation occurred from the cotyledonary node fragments only when it was cultured on germination medium containing BA.There were significant differences in callus induction frequencies among different explants,different concentration ratios of BA and TDZ on callus induction medium.The induction frequency of callus was low for cotyledonary node fragment explants(average less than 20%)under the combination concentration of BA(0.05-0.15 mg·L-1)and TDZ(0.04-0.12 mg·L-1),the highest induction frequency(50%) was obtained when cotyledonary node fragments were cultured on callus induction medium containing 0.15 mg·L-1 BA and 0.08 mg·L-1 TDZ.However,the induction frequency of callus was significantly enhanced by using the cotyledonary node slices as explants(average more than 85%),the highest frequency reached 95%.The callus induced was transferred to differentiation medium containing BA for shoot induction.The highest induction frequency of shoots(21.4%)was obtained on differentiation medium containing 0.2 mg·L-1BA.

Callus

Source

Cite

Citations (0)

Increased Endogenous Methyl Jasmonate Altered Leaf and Root Development in Transgenic Soybean Plants

Journal of genetics and genomics/Journal of Genetics and Genomics (2007)

Ren-Gao Xue Biao Zhang

Methyl jasmonate

Jasmonic acid

Cauliflower mosaic virus

10.1016/s1673-8527(07)60036-8

Cite

Citations (50)

Induction of Multiple Shoots from Cotyledonary Nodes of "Xiaolihuang" Soybean

Dadou kexue (2010)

Ren-Gao Xue

To establish a high frequency regeneration of soybean,the effects of germination day,6-BA and TDZ concentrations on the induction of multi-shoot were studied using cotyledonary nodes ofXiaolihuang as explants in this study.The results showed that the days of germination affected the induction rate of multi-shoot.The highest induction rate of multi-shoot was obtained from the explants of 7-day germinated cotyledonary nodes,which reached 82.6%.With the increasing of the germination day,the induction rate of multi-shoot decreased.Although multi-shoot can be induced from the 1/2MSB5 mediums supplemented with different concentrations of the 6-BA and TDZ,the best induction rate of multi-shoots was occurred when combining of 1.5 mg·L-1 6-BA with 0.2 mg·L-1 TDZ,which reached 85.7%.

Source

Cite

Citations (0)

形質転換の最適化のために,AGROBACTERIUM 介導霞の形質転換を行った。【JST・京大機械翻訳】

Guoshu Xuebao (2008)

Deng Jie Cao Yinsheng Caiyu Liu Xin Liu Ren-Gao Xue

Source

Cite

Citations (0)