Puyuan Tian

Sun Yat-sen University

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Tingtao Chen

Nanchang University

Xiaoxiao Zhao

Zhejiang University

Hua Wei

State Key Laboratory of Food Science and Technology

Hong‐Bo Xin

Nanchang University

Fanjing Meng

First Affiliated Hospital of Xiamen University

Xiaolei Wang

Nanchang University

Huan Wang

Chinese Academy of Sciences

Xianyao Tang

Nanchang University

Chaofei Xia

Nanchang University

Xin Fang

First Affiliated Hospital of Jiangxi Medical College

Cooperative Institutions

Nanchang University

Second Affiliated Hospital of Nanchang University

Sun Yat-sen University

First Affiliated Hospital of Nanchang University

Shanghai Jiao Tong University

Zhengzhou University

Peking University

The First Affiliated Hospital, Sun Yat-sen University

State Key Laboratory of Food Science and Technology

Huazhong Agricultural University

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Reclamation of Herb Residues Using Probiotics and Their Therapeutic Effect on Diarrhea

Mediators of Inflammation (2017)

Fanjing Meng Tingtao Chen Dongwen Ma Xin Wang Xiaoxiao Zhao

Residues from herbal medicine processing in pharmaceutical plants create a large amount of waste (herb residues), which consists mainly of environmental pollution and medicinal waste. In order to resolve this problem, probiotics of Bacillus (B.) subtilis , Aspergillus (A.) oryzae , and Lactobacillus (L.) plantarum M3 are selected to reuse herb residue of Jianweixiaoshi tablets (JT), and an antibiotic-associated diarrhea (AAD) mouse model was established to evaluate the therapeutic effects of the herb residue fermentation supernatant. Our results indicated that the fermentation supernatant had scavenged 77.8% of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 78% of O 2 •− , 36.7% of • OH, 39% of Fe 2+ chelation, and 716 mg/L reducing power. The inhibition zones for Salmonella (S.) typhimurium , S. enteritidis , Shigella (Sh.) flexneri , Escherichia (E.) coli , Listeria (L.) monocytogenes , Sh. dysenteriae 301, and Staphylococcus (S.) aureus were 17, 14, 19, 18, 20, 19, and 20 mm, respectively. The in vivo results indicated that the fermentation supernatant resulted in a high diarrhea inhibition rate (56%,p<0.05), greatly enhanced the disruption of bacterial diversity caused by antibiotics, and restored the dominant position of L. johnsonii in the treatment and recovery stages. Therefore, the combination of the herb residue and probiotics suggests a potential to explore conversion of these materials for the possible development of therapies for AAD.

10.1155/2017/4265898

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Triptolide modulates tumour-colonisation and anti-tumour effect of attenuated Salmonella encoding DNase I

Applied Microbiology and Biotechnology (2018)

Tingtao Chen Xiaoxiao Zhao Yimeng Ren Yuqing Wang Xianyao Tang

Triptolide

Proinflammatory cytokine

10.1007/s00253-018-9481-8

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Preparation and Evaluation of the Fully Humanized Monoclonal Antibody GD-mAb Against Respiratory Syncytial Virus

Frontiers in Cellular and Infection Microbiology (2019)

Puyuan Tian Yuqing Wang Liu Hui Yulu Yang Xiaoli Wu

Respiratory syncytial virus (RSV) is the major cause of pulmonary and bronchial inflammation in infants, young children, and immunocompromised adults, while therapeutic options to control RSV are limited. In the present study, a single chain antibody against RSV (GD-scFv) was screened using phage display library panning technology, and a full-length monoclonal antibody (GD-mAb) was developed based on the sequence encoding Ig VH and Ig VL from GD-scFv. Then, the anti-RSV potential of GD-mAb were evaluated both in vitro and in vivo. Our results indicated that both GD-scFv and GD-mAb possessed a high binding capability and strong binding specificity to GD protein, and plaque reduction neutralization test (PRNT) indicated that GD-mAb could effectively neutralize RSV and reduce plaque number in a concentration-dependent manner in vitro. Moreover, the animal results indicated that GD-mAb had significantly lowered the lung index and reduced the lung virus titer in mice lung (p<0.05), effectively reduced the phosphorylated protein level in pathways of TLR4/NF-κB, MAPKs and PI3K/Akt (p<0.05), which further inhibited the release of pro-inflammatory factors TNF-α, IL-1β and IL-6 in mice lung and serum (p<0.05). These data suggest that the GD-mAb might be used as an effective antiviral agent for RSV infection and treatment.

10.3389/fcimb.2019.00275

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A replication-competent modified vaccinia Tian Tan is superior to modified vaccinia Ankara for inducing neutralizing antibodies via mucosal vaccination

Zhiwei Chen Xueyi Huang Binfeng Lu Qingwei Fang Wei Yu

Replication

Smallpox vaccine

Modified vaccinia Ankara

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Dysbiosis of the rat vagina is efficiently rescued by vaginal microbiota transplantation or probiotic combination

International Journal of Antimicrobial Agents (2021)

Tingtao Chen Chaofei Xia Hong Hu Huan Wang Buzhen Tan

Dysbiosis

Lactobacillus crispatus

Lactobacillus gasseri

10.1016/j.ijantimicag.2021.106277

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Citations (38)

[Construction of mucosal vaccine derived from HBV surface antigen epitope A and the assay of its immunogenicity].

PubMed (2001)

Liu X Guanghui Yang Qiu B Puyuan Tian

The fusion gene of HBV(adr) surface antigen epitope and B subunit of Cholera toxin was constructed and expressed successfully in E. coli at high yield. After denaturation-renaturation process, SDS-PAGE analysis showed that most of the renatured product reassociated in a pentameric form which was the same as natural CTB. Western blot analysis indicated that the immunogenicity of HBsAg antigen epitope was conserved. Moreover, ELISA analysis of the sera of orally, intranasally and intraperitoneally immunized mice showed that the circulating IgG antibodies to HBsAg were developed. The results may be helpful for constructing the novel mucosal vaccine with high efficacy.

Cholera toxin

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Evaluation of truncated G protein delivered by live attenuated Salmonella as a vaccine against respiratory syncytial virus

Microbial Pathogenesis (2018)

Puyuan Tian Di Xu Zhixiang Huang Fanjing Meng Jinheng Fu

Attenuated vaccine

10.1016/j.micpath.2017.12.080

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Neuroprotective effects of an engineered commensal bacterium in the 1‐methyl‐4‐phenyl‐1, 2, 3, 6‐tetrahydropyridine Parkinson disease mouse model via producing glucagon‐like peptide‐1

Journal of Neurochemistry (2019)

Xin Fang Puyuan Tian Xiaoxiao Zhao Chunling Jiang Tingtao Chen

While glucagon-like peptide-1 (GLP-1) was reported to have a positive impact on Parkinson disease, it is extremely short half-life greatly hindered its clinical use. In this study, the mouse strain MG1363-pMG36e-GLP-1 was engineered to continuously express GLP-1 to treat Parkinson disease in a 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated Parkinson disease model. In our study, oral supplementation with MG1363-pMG36e-GLP-1 significantly (p < 0.05) reduced MPTP-induced locomotor impairments, increased tyrosine hydroxylase-positive neurons, suppressed microglia and astrocyte activation, and down-regulated expression of several inflammation-related molecules. In addition, MG1363-pMG36e-GLP-1 significantly (p < 0.01) reduced intestinal pathogen Enterobacteriaceae and markedly enhanced the number of probiotic Lactobacillus and Akkermansia. These data suggest that MG1363-pMG36e-GLP-1 could be a novel therapeutic means for Parkinson disease.

MPTP

10.1111/jnc.14694

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Citations (58)

Evaluation of the accuracy and sensitivity of high‑throughput sequencing technology using known microbiota

Molecular Medicine Reports (2017)

Fanjing Meng Tingtao Chen Xin Wang Xiaolei Wang Hua Wei

Next generation sequencing provides an excellent platform to explore microbiota in any given environment, and little work is required to evaluate the accuracy and sensitivity of high‑throughput sequencing technology. In the present study, a known microbiota containing Escherichia coli, Lactobacillus plantarum, Streptococcus thermophilus, Bifidobacterium bifidum, Bacillus subtilis, Enterococcus faecalis and Salmonella typhimurium was used to evaluate the high‑throughput sequencing technology. The results suggested that there were 122.7 operational taxonomic units (OTUs) in all groups, which is 17.5‑fold (the whole OTU number/the actual bacterial number) greater compared with the actual microbial number in each group, and the Venn method indicated that only 46.38% (64/138), 58.70% (81/138), 86.13% (118/137), 83.57% (117/140) and 89.29% (125/140) of the common OTUs were identified in groups A, B, C, D and E, of which the majority of OTUs did not belong to known bacteria. In addition, the DNA extraction and amplification efficiency failed to identify bacteria at the phylum, class, order, family, genus and species levels, which may further increase false information of microbial analysis. In conclusion, the present study provided basic datato investigate the potential drawbacks of high‑throughput sequencing technology, which will help researchers to avoid exaggerating the bacterial number when this technology is applied to study microbiota in particular environments.

Bifidobacterium bifidum

Enterococcus faecalis

10.3892/mmr.2017.7849

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Citations (14)

Engineered commensal bacteria prevent systemic inflammation-induced memory impairment and amyloidogenesis via producing GLP-1

Applied Microbiology and Biotechnology (2018)

Tingtao Chen Puyuan Tian Zhixiang Huang Xiaoxiao Zhao Huan Wang

10.1007/s00253-018-9155-6

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Citations (42)