Abdolhossein Dalimi

Tarbiat Modares University

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Fatemeh Ghaffarifar

Tarbiat Modares University

112

Majid Pirestani

Tarbiat Modares University

Zohreh Sharifi

High Institute for Education and Research in Transfusion Medicine

Javid Sadraei

Tarbiat Modares University

Amir Abdoli

Jahrom University of Medical Sciences

Fatemeh Ghafarifar

Tarbiat Modares University

Mehdi Mohebali

Tehran University of Medical Sciences

Sayed Hussain Mosawi

Tarbiat Modares University

Vahid Nasiri

Agricultural Research & Education Organization

Zohreh Sharifi

High Institute for Education and Research in Transfusion Medicine

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Tarbiat Modares University

160

Tehran University of Medical Sciences

University of Tehran

Shahid Beheshti University of Medical Sciences

Pasteur Institute of Iran

Razi Vaccine and Serum Research Institute

Iran University of Medical Sciences

Shahid Sadoughi University of Medical Sciences and Health Services

Isfahan University of Medical Sciences

Shiraz University of Medical Sciences

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A Novel Chimeric Antigen as a Vaccine Candidate against Leishmania major: In silico Analysis

Iranian Journal of Parasitology (2021)

Niloofar Bavarsad Ahmadpour Abdolhossein Dalimi Majid Pirestani Javid Sadraei

Leishmania is a mandatory intracellular pathogen and causing neglected disease. Hence, protection against leishmaniasis by a development vaccine is an important subject. This study aimed to design a poly-epitope vaccine for cutaneous leishmaniasis.The present study was conducted in the Parasitology Department of Tarbiat Modares University, Tehran, Iran during 2017-2019. Several bioinformatics methods at online servers were used for prediction of different aspects of poly-epitope, including, physico-chemical attributes, allergenicity, antigenicity, secondary and tertiary structures, B-cell, T-cell and MHC (I, II) potential epitopes of LACK, LEIF, GP63 and SMT antigens of L. major.After designing the construct (GLSL), the outputs of PTM sites demonstrated that the poly-epitope had 57 potential sites for phosphorylation. Furthermore, the secondary of GLSL structure includes 59.42%, 20.94% and 19.63% for random coil, extended strand and alpha-helix, respectively. The GLSL is an immunogenic protein with an acceptable antigenicity (0.8410) and non-allergen. Afterward, 20 potential epitopes of LACK, LEIF, GP63 and SMT antigens were linked by a flexible linker (SAPGTP), then was synthesized, and sub-cloned in pLEXY- neo2. The results were confirmed the expression of 38.7 kDa poly-epitope in secretory and cytosolic sites, separately.A good expression in the L. tarentulae and confirmation of the GLSL poly-epitope could be a basis for developing a vaccine candidate against leishmaniasis that should be confirmed via experimental tests in BALB/c mice.

Antigenicity

Reverse vaccinology

10.18502/ijpa.v16i2.6267

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Phylogenetic Analysis of Toxoplasma gondii Type II and Type III by PCRRFLP Plus Sequencing on Wild-Rats of Golestan Forest, Iran

Journal of Veterinary Science & Technology (2018)

Ehsan Shariat Bahadori Javid Sadraei Abdolhossein Dalimi Somayyeh Namroodi Majid Pirestani

Introduction: Toxoplasma is a genus of intracellular parasites, which was observed in the many types of mammalians. Toxoplasma protozoa that were detected from many types of rats have the equal phenotypic characteristics but have different clinical symptoms and genotypes. Mostly, domestic animals and rodents are the hosts in the reservoirs in the environment. The objective of this article was to phylogenetic identification of toxoplasmosis in wild-rats of Golestan forest using secretory antigens type 1 gene. Materials and Methods: In this surveillance, the researchers gathered 286 wild rodents from Golestan forest and earned DNA from brain and heart tissues to prove SAG1 gene from these hosts. They split these rodents into four groups and then analyzed the positive samples by the Polymerase chain reaction basis. Results: In this surveillance, they discovered 24% of tissues of the wild-rats were infected for these protozoa. Over 55.8% of tissues belonged to Ratus ratus, 17.6% tissues belonged to Ratus norvegicus, 14.7% of tissues belonged to Mus musculus, 11.7% of tissues belonged to Rombumys opimus, also with attention of phylogenetic analysis, 50 infected rats belonged to genotype ΙΙΙ and 18 infected rats belonged to genotype ΙΙ. Conclusion and Discussion: Conclusion showed that different types of wild rats disseminated this type of infection; also SAG1 molecule was a very important molecule to detect toxoplasmosis in rats of Golestan area.

10.4172/2157-7579.1000541

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Determination of Leishmania species causing cutaneous leishmaniasis in Mashhad by PCR-RFLP method

Archives of Razi Institute (2009)

Hossein Vaeznia Abdolhossein Dalimi Javid Sadraei M Pirstani

10.22092/ari.2009.103804

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A study on the cytotoxic effect of cantharidin on Leishmania major promastigote and amastigote survival in vitro

KAUMS Journal (2012)

Yahya Maroufi Fatemeh Ghaffarifar Abdolhossein Dalimi Zohreh Sharifi Zahir Mohammad Hasan

Amastigote

Cantharidin

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Apoptotic Effect of Morphine, Imiquimod and Nalmefene on Promastigote, Infected and Uninfected Macrophages with Amastigote of Leishmania Major by Flow Cytometry.

PubMed (2018)

Parisa Ebrahimisadr Fatemeh Ghaffarifar John Horton Abdolhossein Dalimi Zohreh Sharifi

The parasites of genus Leishmania are the causative agents of one of the most widespread and devastating diseases. According to follow-up data, these medications may provoke adverse drug reactions, drug resistance, relapse, as well as financial burden. The mechanism of action of opioid drugs is primarily exerted via transmembrane G-protein coupled receptors. One of the potent synthetic immunomodulator agents is imiquimod with low molecular weight and unknown mechanism of action. Monocyte and macrophage are the primary site of action for imiquimod. Nalmefene is a well-known opioid antagonist agent which simultaneously inhibits these receptors and augments intracellular pathogenicity, hence providing opportunities to investigate their function. The aim of present work was evaluating the effect of morphine, imiquimod and nalmephen on the Leishmania major and investigating cytotoxic effect this drug on the uninfected macrophage and infected macrophage for detected early apoptosis, necrosis and secondry apoptosis by flowcytometry method. In this study we used morphine, imiquimod, nalmefene, and Glucantime. We treated promastigotes, macrophages, and infected macrophages with above drugs, and the apoptosis evaluated by flow cytometry. The results showed that in all concentration of morphine more than 98% of promastigotes remained alive that it is deduced that morphine lacks any lethal effect on L. major after 24 h, whereas in groups treated with Glucantime alone or in combination with Nalmephene and Imiquimod, 84.13%, 88.96% and 86.72% of promastigotes were alive, respectively. The results of macrophage treatment with morphine, imiquimod, and nalmefene demonstrated that most necrosis has occurred in nalmefene group (6.54%).

Imiquimod

Nalmefene

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Genotyping of Acanthamoeba Isolated from Hospital Environments and Thermal Water of Recreational Baths in Markazi Province, Iran

Iranian Journal of Parasitology (2023)

Alireza Mohammady Abdolhossein Dalimi Fatemeh Ghafarifar Majid Akbari Majid Pirestani

Due to the opportunism character of Acanthamoeba, the presence of this parasite in the thermal water of recreational baths and hospital environments can be a risk to the health of staff, patients and others. The aim of this study was to investigate the distribution of potentially pathogenic Acanthamoeba genotypes isolated from the hospital environment and the thermal water of recreational baths in Markazi Province, central Iran.Overall, 180 samples including thermal water from recreational baths in Mahallat City and dust, soil and water from different hospitals of Arak, Farahan and Komijan cities, central Iran were collected. The presence of Acanthamoeba was investigated using microscopic examination and molecular methods. The PCR and sequencing was performed based on a specific 18S fragment of ribosomal DNA.Based on the microscopic survey, totally 134 positive samples were detected including 35% in thermal water samples and 44.7% in hospital samples. In molecular analysis, 53.5% of the samples were identified as Acanthamoeba and 46.7% as Protacanthamoeba bohemica. The genotypes were detected as T4 (33.3%), T2 (10%), T11 (6.7%), and T5 (3.3%).The T4 was the most common genotype found in hospitals sampling sites while the T2 genotype and P. bohemica were detected in thermal water sampling sites.

10.18502/ijpa.v18i1.12378

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Clinical and molecular features of human cystic echinococcosis in Tehran, Iran, 2011-2019

Asian Pacific Journal of Tropical Medicine (2022)

Narges Amani Hamedani Majid Pirestani Abdolhossein Dalimi

Objective: To evaluate the clinical and molecular features of human cystic echinococcosis in Tehran, Iran. Methods: In this cross-sectional study, all human cystic echinococcosis patients admitted to Tehran’s hospitals from 2011 to 2019 were enrolled, and demographic characteristics, clinical findings, and laboratory data were collected. Formalin-fixed, paraffin-embedded and fresh tissue samples of 175 cystic echinococcosis patients were evaluated for molecular characterization. The samples’ isolated DNA was used to amplify cytochrome c oxidase I and NADH dehydrogenase subunit I genes. Also, the morphological features of fresh samples were examined. Results: In total, 175 patients with a mean age of 45 (9-98) years were admitted to Tehran’s hospitals diagnosed with cystic echinococcosis. Moreover, the highest (26.9%) and the lowest (2.9%) prevalence of cystic echinococcosis cases were in the 16-30 and 1-15 years range, respectively. Male/female ratio was 0.96 (49.1% vs . 50.9%). The liver was affected in 92 patients (52.6%), and two or more organs were infected in 7 patients (4.0%). The cysts’ diameter varied from 1 to 25 cm, and 96.0% of the patients had a single hydatid cyst. All patients underwent radical surgery and the PAIR technique was applied for all cases. No significant difference was observed between the protoscolex hooks of pulmonary and hepatic cysts. G1-G3 had the highest percentage (99.4%) over other identified G6 genotypes (0.6%). Conclusions: The sheep-dog cycle plays an important role in transmitting the human cystic echinococcosis infection in Tehran.

Cystic echinococcosis

Echinococcus

10.4103/1995-7645.364002

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A review of the effect of medicinal plant extracts on Leishmania major in vitro and in vivo conditions in Iran

Afghanistan Journal of Infectious Diseases (2024)

Hermineh Gholizadeh Abdolhossein Dalimi Fatemeh Ghaffarifar

Background: Leishmaniasis is one of the most common parasitic diseases in tropical and subtropical regions of the world and is considered a threat to public health. Iran is also one of the most endemic areas of cutaneous leishmaniasis in the world. The causative species of cutaneous leishmaniasis is a protozoan from the Kinetoplastida order, which in Iran is Leishmania major (rural type) and Leishmania tropica (urban type). More than 70% of leishmaniasis in Iran is Leishmania major. The reservoir of the disease is humans in the urban type and field rodents in the rural type, and the vector is the female mosquito of the genus Phlebotomus. Methods: This study is organized as a review, in which, by searching the keywords cutaneous leishmaniasis, Leishmania major, plants effective against leishmaniasis in Iran, scientific-research articles, Google Scholar search engine information, Pubmed and Science Direct databases, the available books in this field were analyzed. Results: 5-valent antimoan compounds are used for the treatment of cutaneous leishmaniasis. Of course, not all patients need treatment because, in a large number of people, the lesion heals by itself, and due to the side effects of antimoan compounds, it is better to use fewer of these drugs. Therefore, the desire of patients and therapists to use herbal compounds has increased. Conclusion: Although cutaneous leishmaniasis is not usually associated with high Although cutaneous leishmaniasis is not usually associated with high mortality, the rate of infection is very high and causes malformed skin lesions that remain for more than a year in some cases, and even with standard treatment, scars remain. It remains forever and causes emotional pain for the patient. Therefore, domestic researchers have provided research on herbal treatments against Leishmaniasis, considering the history of traditional treatments in Iran and the scattered vegetation in the country.

Leishmania tropica

Tropical disease

Neglected Tropical Diseases

10.60141/ajid/v.2.i.1.10

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Neuropsychiatric manifestations of latent toxoplasmosis on mothers and their offspring

The Journal of Maternal-Fetal & Neonatal Medicine (2013)

Amir Abdoli Abdolhossein Dalimi Mohsen Arbabi Fatemeh Ghaffarifar

Toxoplasmosis is one of the most common parasitic diseases worldwide. It is estimated that approximately one-third of the world's population is latently infected. Infection generally occurs via oral the route and maternal transmission. Damage of the central nervous system is one of the most serious consequences of congenital toxoplasmosis. Moreover, recent investigations proposed that acute and sub-acute congenital toxoplasmosis as well as latent toxoplasmosis during pregnancy; play various roles in the etiology of different neuropsychiatric disorders in mothers and their offspring. This paper reviews new findings about the role of latent toxoplasmosis in the etiology of various neuropsychiatric disorders in mothers and their offspring.

Toxoplasmosis

Etiology

Congenital toxoplasmosis

10.3109/14767058.2013.858685

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Citations (32)

In Silico Analysis of the ROP29 Protein as a Vaccine Candidate Against Toxoplasma gondii

Journal of Parasitology Research (2024)

Amir KarimiPourSaryazdi Fatemeh Ghaffarifar Abdolhossein Dalimi Masoud Foroutan John Horton

The progression of Toxoplasma gondii ( T. gondii ) invasion is aided by rhoptry proteins (ROPs), which are also crucial for the parasite’s survival in host cells. In this study, in silico analysis was performed to examine the various aspects of the ROP29 protein, such as physicochemical properties, potential T‐ and B‐cell epitopes, and other significant features. The research revealed that there were 55 possible sites for posttranslational modification in the ROP29 protein. The secondary structure of the ROP29 protein consists of a random coil, an alpha‐helix, and an extended strand, which account for 49.69%, 36.81%, and 13.50%, respectively. Moreover, a number of putative T‐ and B‐cell epitopes for ROP29 were found. The Ramachandran plot showed that 88.91% (crude model) and 97.54% (refine model) of the amino acid residues were located in the favored regions. Also, the testing of this protein’s antigenicity and allergenicity showed that it was nonallergenic and immunogenic. Our results suggested that employing in silico tools to apply structural and functional predictions to the ROP29 protein can lower the likelihood that laboratory investigations will fail. This research served as a crucial foundation for further research. More research is required in the future in suitable animal model employing ROP29 alone or in combination with other antigens.

Apicoplast

10.1155/2024/1918202

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Citations (1)