SUMMARY: Compartmentation phenomena were studied in the course of the simultaneous metabolism of glucose, galactose and lactose by organisms of Escherichia coli which were induced for either the lac operon, the gal operon, both, or neither. Metabolic patterns were investigated in each phenotype by incubating parallel identical cultures with the three sugars in equal chemical concentration but labelled differently with 14C. The four labelled substrates were glucose, galactose and lactose labelled either exclusively in the glucose moiety or exclusively in the galactose moiety. The metabolites from free glucose in the medium equilibrated with those from free galactose in the medium, but did not equilibrate with metabolic products derived from glucose generated endogenously by the hydrolysis of lactose. Similarly, metabolic products derived from galactose formed in the hydrolysis of lactose equilibrated with those from glucose from the same source, but not with metabolic intermediates formed from either free glucose or free galactose in the medium. Other interpretations of these results, not involving metabolic compartmentation, have been considered and found inadequate to account for the observed results. Some of the implications of compartmentation in bacteria are discussed.

Carbohydrate Metabolism

Metabolic pathway

gal operon

10.1099/00221287-51-2-159

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Eicosanoids, Lipid Peroxidation and Cancer

Springer eBooks (1988)

Santosh Nigam D. C. H. McBrien Trevor F. Slater

10.1007/978-3-642-73424-3

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Citations (66)

Biochemical mechanisms of platinum antitumour drugs : based on the proceedings of a symposium held at Brunel University, 3-5th July 1985

IRL Press eBooks (1986)

D. C. H. McBrien Timothy F. Slater

Preface Participants Non-participating co-authors Introduction Antitumour activity Metabolism Renal toxicity Clinical survey Analytical techniques General discussion Keywork index

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The inhibition of renal ATPase by cisplatin and some bio-transformation products

Chemico-Biological Interactions (1982)

Peter T. Daley‐Yates D. C. H. McBrien

Nephrotoxicity

10.1016/0009-2797(82)90155-7

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Citations (78)

The Effect of Methyl Glyoxal on Cell Division and the Synthesis of Protein and DNA in Synchronous and Asynchronous Cultures of Escherichia coli B/r

Microbiology (1980)

H.N.A. Fraval D. C. H. McBrien

Methyl glyoxal inhibits the growth of Escherichia coli in synchronous and asynchronous culture. The inhibition of growth is accompanied by immediate inhibition of protein synthesis and of the initiation of replication of DNA. When methyl glyoxal is added after initiation of a round of replication the elongation of new DNA chains is not inhibited. Cell division is inhibited if methyl glyoxal is added up to about 22 min prior to division. These results support the view that the primary effect of methyl glyoxal is on protein synthesis.

Glyoxal

Elongation

10.1099/00221287-117-1-127

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Citations (41)

Effect of isopropylthiogalactoside on induction of the galactose operon by D-fucose in a lactose deletion mutant of Escherichia coli

Journal of Bacteriology (1966)

D. C. H. McBrien V. Moses

gal operon

L-arabinose operon

Lac repressor

trp operon

10.1128/jb.91.3.1391-1392.1966

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Citations (5)

Free Radicals, Lipid Peroxidation and Cancer

D. C. H. McBrien T. F. Slater

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Citations (320)

Protective Agents in Cancer

D. C. H. McBrien T. F. Slater

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Citations (98)

The Effect of Toxic Doses of Copper upon Respiration, Photosynthesis and Growth of Chlorella vulgaris

Physiologia Plantarum (1967)

D. C. H. McBrien Kenneth A. Hassall

Abstract When cells of Chlorella vulgaris absorb copper under anaerobic conditions, subsequent respiration, photosynthesis and growth of the cells are all severely inhibited. This does not occur when the metal is absorbed under aerobic conditions. When, after aerobic absorption of copper, the cells are exposed to a period of anaerobiosis, respiratory inhibition is as profound as when the uptake is anaerobic. In this case, however, respiration must eventually recover, for growth is not affected so severely as it is when copper is taken up under anaerobic conditions. It is concluded that the extra copper absorbed under anaerobic conditions is directly or indirectly responsible for the greatly increased toxicity to growth, and that this copper is bound to sites not normally available under aerobic conditions. Some aspects of the apparently unique toxic effect of copper suggest that these extra sites are sulphydryl groups.

Chlorella vulgaris

Cellular respiration

Anaerobic respiration

Copper toxicity

10.1111/j.1399-3054.1967.tb07148.x

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Citations (49)