Tvrtko Smital

Rudjer Boskovic Institute

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RŽ

Roko Žaja

University Hospital Bonn

Jovica Lončar

Rudjer Boskovic Institute

Marta Popović

Rudjer Boskovic Institute

Ivan Mihaljević

Rudjer Boskovic Institute

Marijan Ahel

Rudjer Boskovic Institute

Senka Terzić

Rudjer Boskovic Institute

Jelena Dragojević

Rudjer Boskovic Institute

Roberta Sauerborn Klobučar

Rudjer Boskovic Institute

Ivan Senta

Rudjer Boskovic Institute

Sanja Krča

Rudjer Boskovic Institute

Cooperative Institutions

Rudjer Boskovic Institute

University of Zagreb

Institute for Medical Research and Occupational Health

University of Novi Sad

Johannes Gutenberg University Mainz

University of Belgrade

University of Osijek

Stanford University

Moss Landing Marine Laboratories

Pacific University

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International round‐robin study on the Ames fluctuation test

Environmental and Molecular Mutagenesis (2012)

Georg Reifferscheid H. Maes B. Allner Jana Badurová Shimshon Belkin

Abstract An international round‐robin study on the Ames fluctuation test [ISO 11350, 2012], a microplate version of the classic plate‐incorporation method for the detection of mutagenicity in water, wastewater and chemicals was performed by 18 laboratories from seven countries. Such a round‐robin study is a precondition for both the finalization of the ISO standardization process and a possible regulatory implementation in water legislation. The laboratories tested four water samples (spiked/nonspiked) and two chemical mixtures with and without supplementation of a S9‐mix. Validity criteria (acceptable spontaneous and positive control‐induced mutation counts) were fulfilled by 92–100%, depending on the test conditions. A two‐step method for statistical evaluation of the test results is proposed and assessed in terms of specificity and sensitivity. The data were first subjected to powerful analysis of variance (ANOVA) after an arcsine‐square‐root transformation to detect significant differences between the test samples and the negative control (NC). A threshold (TH) value based on a pooled NC was then calculated to exclude false positive test results. Statistically, positive effects observed by the William's test were considered negative, if the mean of all replicates of a sample did not exceed the calculated TH. By making use of this approach, the overall test sensitivity was 100%, and the test specificity ranged from 80 to 100%. Environ. Mol. Mutagen. 2012. © 2012 Wiley Periodicals, Inc.

Round robin test

Ames test

Negative control

Positive control

10.1002/em.21677

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Citations (80)

Inhibitors of the ABC transport proteins as emerging pollutants—Determination and ecotoxicological relevance

Toxicology Letters (2006)

Tvrtko Smital David Epel Smiljana Britivic Sanja Krča Branka Pivčević

Nutraceutical

Betulinic acid

Efflux

Terpene

10.1016/j.toxlet.2006.06.092

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Citations (2)

Ecotoxicological Characterization of the Sava River: Biomarker Responses and Biological Assays

The handbook of environmental chemistry (2014)

Tvrtko Smital Marijan Ahel

Human settlement

10.1007/978-3-662-44034-6_8

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Citations (10)

Hepatic biomarker responses to organic contaminants in feral chub (Leuciscus cephalus)—laboratory characterization and field study in the Sava River, Croatia

Environmental Toxicology and Chemistry (2007)

Sanja Krča Roko Žaja Violeta Čalić Senka Terzić Miloš Saša Grubešić

Abstract As a widely spread cyprinid fish species, the European chub ( Leuciscus cephalus ) has been used extensively in bio‐monitoring programs. However, no laboratory dose‐response and/or time course studies related to applied biomarkers have been reported on chub yet. In order to address this issue, specimens of juvenile chub caught in September 2005 in the Sava River, Croatia, were laboratory exposed to various (0.25–50 mg/kg) doses of either model polycyclic aromatic hydrocarbons (PAH) premutagen benzo[ a ]pyrene (B a P), or β‐naphthoflavone (β‐NF), a well‐known model cytochrome 1A (CYP1A) inducer, for 3 (B a P) or 5 d (β‐NF). The responses of several hepatic biomarkers were determined in the exposed fish: The hepatic 7‐ethoxyresorufin‐O‐deethylase activity, CYP1A content, glutathione S ‐transferase (GST) activity, liver bioactivation potential, and the amount of hydroxylated polycyclic aromatic hydrocarbon bile metabolites determined by the fixed wavelength fluorescence and the high‐performance liquid chromatography technique. The relevance of determined biomarker responses has been analyzed further and crosscorrelated with the same set of biomarkers, as well as with tissue concentrations of polycyclic aromatic hydrocarbons and polychlorinated biphenyls, determined in chub specimens collected in September 2005 at five different polluted locations along the Sava River. The species‐specific upper and lower limits in responses of studied biomarkers were determined and the obtained ranges successfully evaluated in real field situation. With the exception of the GST activity, all other biomarkers determined in chub proved to be valuable indicators of environmental pollution. Finally, the results of the present study demonstrated that the same strategy of laboratory characterization in combination with field evaluation should be used regularly in the selection of optimal biomarkers and indicator species.

Leuciscus

Polycyclic aromatic hydrocarbon

10.1897/07-227.1

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Citations (40)

Interspecies differences in P-glycoprotein mediated activity of multixenobiotic resistance mechanism in several marine and freshwater invertebrates

Comparative Biochemistry and Physiology Part C Pharmacology Toxicology and Endocrinology (2000)

Tvrtko Smital Roberta Sauerborn Branka Pivčević Sanja Krča Branko Kurelec

Dreissena

Marine invertebrates

10.1016/s0742-8413(00)00110-9

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Citations (78)

The first demonstration of CYP1A and the ABC protein(s) gene expression and activity in European seabass (Dicentrarchus labrax) primary hepatocytes

Chemosphere (2013)

Marta Ferreira Pedro Miguel Gouveia Santos Ledicia Rey‐Salgueiro Roko Žaja M.A. Reis-Henriques

Dicentrarchus

Detoxification

Xenobiotic

Rhodamine 123

10.1016/j.chemosphere.2013.11.051

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Citations (20)

An integrated environmental monitoring system for Croatian freshwater, estuarine and coastal marine areas : the CROWAT project

Biserka Raspor Marijana Erk Vlatka Filipović Jasenka Pavičić Dušica Jurič

Hrvatska je bogata vodom, ali zbog nedostatka postrojenja za njeno prociscavanje velike kolicine otpadnih voda iz gradova i industrijskih postrojenja uzrokuju degradaciju njene kakvoce. Sprecavanje gubitka naseg prirodnog bogatstva iziskuje razvoj i uspostavu specificnih monitoring programa za procjenu zdravlja bioloske komponente i kakvoce voda. Glavni cilj CROWAT je, suradnjom hrvatskih i norveskih istraživaca osmisliti, razviti i uspostaviti biomonitoring hrvatskih kopnenih voda i mora. U ovom interdisciplinarnom projektu upotrebljavaju se vecinom bioloske metode koje odražavaju promjene u organizmima uzrokovane djelovanjem oneciscivaca - biomarkere. Biomarkeri, kao mjera izlaganja oneciscenju i njegovog ucinka su od posebnog znacaja jer reagiraju samo na bioloski raspoložive koncentracije oneciscivaca i pružaju mogucnost rane detekcije promjena uzrokovanih oneciscenjem. Tijekom prve godine projekta (2002) za nasa istraživanja analizirali smo prirodne populacije organizama (skoljkase i ribe) kao i jedinke izlagane u kavezima. Istraživanja su provedena na tri postaje na Srednjem Jadranu (Kastelanski zaljev) i tri postaje u Istocnoj Slavoniji (rijeke Drava i Dunav). Ovaj projekt ukljucuje mjerenje slijedecih biomarkera: koncentracije metalotioneina, aktivnosti etoksirezorufin-O-deetilaze (EROD), aktivnosti benzo(a)piren monooksigenaze (BaPMO), aktivnosti acetilkolinesteraze (AChE), aktivnosti mehanizma multiksenobioticke otpornosti (MXR), koncentracije glutationa (GSH), koncentracije vitelogenina, ostecenja DNA (komet-test i mikronukleus-test), koncentracije policiklickih aromatskih ugljikovodika i njihovih metabolita, energetskog statusa organizama i destabilizacije lizosomalne membrane. Ovaj projekt (br. 150-463) financiran je od strane Norweigan Research Council-a.

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Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys

Archives of Industrial Hygiene and Toxicology (2018)

Dean Karaica Davorka Breljak Jovica Lončar Mila Lovrić Vedran Micek

Abstract Chloride/formate exchanger (CFEX; SLC26A6) mediates oxalate transport in various mammalian organs. Studies in Cfex knockout mice indicated its possible role in development of male-dominant hyperoxaluria and oxalate urolithiasis. Rats provide an important model for studying this pathophysiological condition, but data on Cfex (rCfex) localisation and regulation in their organs are limited. Here we applied the RT-PCR and immunochemical methods to investigate rCfex mRNA and protein expression and regulation by sex hormones in the pancreas, small intestine, liver, and kidneys from intact prepubertal and adult as well as gonadectomised adult rats treated with sex hormones. rCfex cDNA-transfected HEK293 cells were used to confirm the specificity of the commercial anti-CFEX antibody. Various biochemical parameters were measured in 24-h urine collected in metabolic cages. rCfex mRNA and related protein expression varied in all tested organs. Sex-independent expression of the rCfex protein was detected in pancreatic intercalated ducts (apical domain), small intestinal enterocytes (brush-border membrane; duodenum > jejunum > ileum), and hepatocytes (canalicular membrane). In kidneys, the rCfex protein was immunolocalised to the proximal tubule brush-border with segment-specific pattern (S1=S2<S3), and both rCfex mRNA and protein expression exhibited male-dominant sex differences driven by stimulatory effects of androgens after puberty. However, urinary oxalate excretion was unrelated to renal rCfex protein expression. While the effect of male-dominant expression of rCfex in renal proximal tubules on urine oxalate excretion remains unknown, its expression in the hepatocyte canalicular membrane may be a pathway of oxalate elimination via bile.

Brush border

10.2478/aiht-2018-69-3157

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Citations (6)

Multixenobiotic resistance mechanism in honeybees

Marica Maja Dražić Tvrtko Smital Smiljana Britvić Budicin Sanja Krča Dalibor Jelaska

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CROWAT projekt : sustav monitoringa okoliša za kopnene vode, estuarije i more u Hrvatskoj

Biserka Raspor Marijana Erk Vlatka Filipović Jasenka Pavičić Dušica Jurič

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