K. Sames

Max Delbrück Center

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Els J. M. Van Damme

Ghent University

P. Schmiegelow

Städtisches Klinikum Solingen

Udo Schumacher

University Cancer Center Hamburg

Gerhard Hommel

Zimmer Biomet (United States)

R. Wobst

Friedrich-Alexander-Universität Erlangen-Nürnberg

Willy J. Peumans

Ghent University

Johannes W. Rohen

Deutsche Ophthalmologische Gesellschaft

Zdeněk Halata

Charles University

G. J. Naylor

NHS Greater Glasgow and Clyde

Jaroslav Lindner

Charles University

Cooperative Institutions

Universität Hamburg

University Medical Center Hamburg-Eppendorf

Freie Universität Berlin

Friedrich-Alexander-Universität Erlangen-Nürnberg

Heidelberg University

Philipps University of Marburg

University of Dundee

Charité - Universitätsmedizin Berlin

Centre National de la Recherche Scientifique

Ninewells Hospital

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Embryotoxic effects of thalidomide-derivatives in the non-human primate Callithrix jacchus

Archives of Toxicology (1988)

Hans‐Joachim Merker Wolfgang Heger K. Sames Helga St�rje Diether Neubert

Skeleton (computer programming)

10.1007/bf00316631

Cite

Citations (50)

PubMed (1974)

K. Sames Th. Stegmann W. Rebel

Human heart

Source

Cite

Citations (2)

Author Index

Gerontology (1980)

E. L. Levitsky G. J. Naylor E. G. Dick Anne H. W. Smith D. A. T. Dick

10.1159/000212439

Cite

Citations (0)

Recent Articles in Ageing Research

Gerontology (1974)

K. Sames Thomas Stegmann W. Rebel W Remy H Bockendahl

As a regular feature of Gerontología, the Editors publish in each issue a selection of recent articles in all fields of ageing research covered by this journal, appearing in other journals in this field and in some multi-disciplinary journals generally accessible to most readers.The selection does not intend to be complete, but to present articles which, in the opinion of the Editors, will be of interest to our readers.The Editors are responsible for the selection, but invite readers to bring to

10.1159/000212006

Cite

Citations (0)

Mechanisms of Ageing and Development (1983)

Heinz Horstmann Johannes W. Rohen K. Sames

Cyanogen bromide

Hydroxyproline

Pepsin

Hydroxylysine

10.1016/0047-6374(83)90069-6

Cite

Citations (45)

Lectin and Proteoglycan Histochemistry of Feline Pacinian Corpuscles

Journal of Histochemistry & Cytochemistry (2001)

K. Sames Zdeněk Halata Milan Jojović Els J. M. Van Damme Willy J. Peumans

We studied carbohydrate residues of glycoproteins and proteoglycans (PGs) in peritoneal Pacinian corpuscles of five adult cats. Terminal monosaccharides of glycoproteins and related polysaccharides were identified by lectin histochemistry and the PGs and glycosaminoglycans (GAGs) by specific antibodies. The most intensive lectin staining reactions indicated an abundance of glycoconjugates with terminal mannose (Man) or sialic acid residues, but no complex-type oligosaccharides were detected within the corpuscles. Terminal fucose (Fuc) and galactose (Gal) residues typical for O-linked mucin-type glycoproteins generally associated with high water binding capacity were also absent. Antibodies against unsulfated chondroitin (C-0-S), chondroitin-4-sulfate (C-4-S), and decorin showed positive reactions in the interfibrillar spaces between the lamellae, around collagen fibers, and around the lamellae of the perineural capsule, especially in the outer parts known to contain Type II collagen. Biglycan showed a preference for the innermost part of the perineural capsule (intermediate layer), known to contain Type V collagen. Collagen V and biglycan are both linked to growth processes. Hyaluronic acid (HA), chondroitin-6-sulfate (C-6-S) chains, and a chondroitin sulfate proteoglycan (CSPG) were co-localized in the terminal glia. The study of carbohydrates with high water binding capacity may contribute to our understanding of the high viscoelasticity of Pacinian corpuscles.

Biglycan

Chondroitin

Glycoconjugate

Keratan sulfate

10.1177/002215540104900103

Cite

Citations (18)

Changes of the glycosaminoglycan metabolism in human lung fibroblasts and porcine aortic endothelium cells influenced by the number of subcultures in vitro and by lipids in the medium.

PubMed (1991)

K. Sames Brett L. Lucht

In vitro aging fibroblasts and porcine aortic endothelium cells were treated with a commercial soy-bean lipid emulsion and incubated in 35S-sulfate or 3H-glucosamine respectively. The glycosaminoglycans (GAGs) were characterized by enzyme digestion following proteolysis. Uronic acid concentrations were determined in fibroblast cultures. The GAG content is lower in the last possible passage than in the foregoing ones. Lipid treatment leads to an increase, especially in early passages. The incorporation of radiolabeled precursors decreases with increasing in vitro age in both cell types. By lipid treatment the heparansulfate (HS) radioactivity of fibroblasts is increased insignificantly, while the GAG radioactivity of endothelium cells and the chondroitinsulfate (CS) radioactivity of fibroblasts are decreased. The in vitro age-related reduction of the precursor incorporation is more pronounced in CS as compared to HS, whereby the HS radioactivity shows a percentual increase, especially following lipid treatment. The effect of lipids shows changes related to in vitro aging. The role of cellular GAG metabolism and lipid loads in arteriosclerosis is discussed. Even in the arterial wall different cell types may react differentially.

Source

Cite

Citations (2)

Histochemical demonstration of acidic glycosaminoglycans in the cell nuclei of the iris and other tissues

Cells Tissues Organs (1979)

K. Sames

Using histochemical methods, the presence of acidic glycosaminoglycans in the cell nuclei of 51 human irides and a series of monkey organs was demonstrated. In general, these substances are sensitive to testicular hyaluronidase and chondroitinase ABC and also to Streptomyces hyaluronidase, when using special staining methods. The specificity of testicular hyaluronidase was tested by. inhibition with heparin. By simultaneously staining with alcian blue and Feulgen, acidic glycosaminoglycans can be distinguished from the nucleic acids. Sporadically, hyaluronidase-resistant substances with a specific acidic glycosaminoglycan stainability occur. We assume the existence of various acidic glycosaminoglycans in the cell nuclei. Aging changes were not traceable with constancy.

Hyaluronidase

Feulgen stain

10.1159/000144997

Cite

Citations (5)

Histochemistry of Glycoconjugates in Merkel Cell Carcinomas

Springer eBooks (2003)

K. Sames Udo Schumacher R. Moll Ingrid Moll Zdeněk Halata

Merkel cell

10.1007/978-3-662-10358-6_28

Cite

Citations (0)