N. V. Vasilyeva

North-Western State Medical University named after I.I. Mechnikov

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НН

Н Н Климко

North-Western State Medical University named after I.I. Mechnikov

ЕВ

Е. В. Фролова

North-Western State Medical University named after I.I. Mechnikov

ТС

Т С Богомолова

North-Western State Medical University named after I.I. Mechnikov

АЕ

А Е Учеваткина

North-Western State Medical University named after I.I. Mechnikov

ЛВ

Л. В. Филиппова

North-Western State Medical University named after I.I. Mechnikov

ОВ

О. В. Шадривова

Ministry of Health of the Russian Federation

Yana I. Kozlova

North-Western State Medical University named after I.I. Mechnikov

АЕ

А. Е. Тараскина

North-Western State Medical University named after I.I. Mechnikov

Anastasia E. Taraskina

First Pavlov State Medical University of St. Petersburg

Ivan M. Pchelin

Czech Academy of Sciences, Institute of Experimental Medicine

Cooperative Institutions

North-Western State Medical University named after I.I. Mechnikov

Ministry of Health of the Russian Federation

First Pavlov State Medical University of St. Petersburg

Pirogov Russian National Research Medical University

Russian Academy of Sciences

Sechenov University

Research Centre for Medical Genetics

Harbin Medical University

St Petersburg University

City Hospital No. 40

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Single-nucleotide polymorphism in a local population of Trichophyton rubrum

Medical Mycology (2017)

Ivan M. Pchelin Daniil V. Azarov Г А Чилина Kirill Dmitriev N. V. Vasilyeva

Trichophyton rubrum is an important causative agent of superficial mycoses worldwide. To uncover a genetic composition of a local population of this fungus, we sequenced A7C99_6411 and A7C99_6714 loci, coding for hypothetical proteins and revealed two complex genotypes, differing by a single missense mutation in each locus. One of the two A7C99_6411/6714 genotypes was not found in tinea pedis cases and demonstrated mostly longer TRS-1 elements when compared to another genotype. Thus, we present a description of nucleotide polymorphism in protein-coding loci in T. rubrum and provide evidence for ecological preferences of T. rubrum genotypes at a local scale.

10.1093/mmy/myx009

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Indices of cellular and humoral immunity in a patient with a history of central precocious puberty in anamnesis

Russian Journal of Immunology (2023)

Diliara R. Khisamutdinova Ya. I. Kozlova Elena Bashnina Е. В. Фролова А Е Учеваткина

The etiology of precocious puberty includes organic anomalies, genetic mutations, but the primary cause remains unclear in the vast majority of cases. Gonadotropin-releasing hormone (GRH) agonists are used as a treatment of gonadotropin-dependent precocious puberty. Blocking the secretion of gonadotropin-releasing hormone, these drugs stop the premature development of sexual features, prevent premature closure of ossification zones, thereby increasing the child’s expected adult height. The interest in the effects of this group of drugs beyond the hypothalamic-pituitary-gonadal axis has been recently increased. A series of clinical cases have been reported on the development of autoimmune diseases, e.g., autoimmune thyroiditis, Graves disease and type 1 diabetes. The article presents a clinical observation of a patient with central form of premature development who exhibited satisfactory response to treatment with a GRH agonist drug. Further follow-up did not show any reproductive dysfunction. Upon immunological examination, a disturbance was revealed only in the cellular component of immunity. An increased metabolic activity of neutrophils was found, thus, probably, indicating a nonspecific inflammatory process. The levels of immunoglobulins A, M, G matched the reference values. Thus, the therapy with a drug from the group of GRH agonists was effective and safe in terms of influencing the patient’s immune system. The role of hormonal disorders and effects of GRH agonists on the development of immunopathological conditions require further research.

Anamnesis

Etiology

Gonadotropin

10.46235/1028-7221-13906-ioc

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USE OF FISH FEED COMPLEX AND PINE CONE HUSKS IN THE DIETS OF YOUNG LAYING HENS IN THE PRIMORSKY REGION

Bulletin of KSAU (2021)

Zoya Tsoy N. V. Vasilyeva

Husk

Laying

Cone (formal languages)

10.36718/1819-4036-2021-3-93-96

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Hygienic safety problems of baby bath toys

Hygiene and Sanitation (2022)

N. V. Vasilyeva T. S. Bogomolova O.G. Khurtsilava И. Ш. Якубова L.A. Alikbaeva

Introduction. There is a potential risk of harm to kid health when using toys, in particular, there is a possibility of micro-fungi entering the oral cavity of a baby when using bath toys, which may be the cause of mycotic diseases. The study was initiated by the appeal of the Russian TV First Channel to the Institute of Medical Mycology named after P.N. Kashkin, where samples of used bath toys with a black scurf inside were transferred for research and evaluation of their safety. The purpose: to evaluate the safety of the presented samples of baby bath toys after their intended use. Materials and methods. The experimental group was represented by four baby toys for the bath, which were used for their intended purpose, and were operated for several months. As a control, 4 similar toys for the bath were used. The study of the inner surface of the toys of the experimental group was carried out using a Leica DM 4020 light microscope,and sowing scrapings on Sabouraud’s solid nutrient medium. Isolates were determined by MALDI-TOF mass spectrometry. The inner surface of the toys was also studied by scanning electron microscopy. The fungus resistance of the samples was assessed according to GOST 9.049-91. Results. The study of the inner surface of prototype toys for the bath established the well-developed and “young” biofilms in the black areas of the inner surface. Biconvex spores (2×3 μm) of fungal origin were identified. Inoculation of swabs from the inner surface revealed a massive growth of opportunistic fungi: yeast fungi Candida guilliermondii (Castell.) Langeron & Guerra and Rhodotorula spp.. Studies on the fungus resistance of control samples of toys show that the material is not a nutrient medium for microfungi (neutral or fungistatic). Limitations: a relatively small sample of samples, the use of expensive high-tech equipment, the difficulty of reproducibility of the result. Conclusion. Colonization of the interior surface of toy specimens used in bathing kids with microscopic fungi and bacteria is indicative of a potential risk of harm to infants due to product design, period of use, and possibly materials used. In this regard, it is necessary to review the hygienic safety requirements for the design of bath toys and make appropriate additions to the Technical Regulations of the Customs Union “On the safety of toys” (TR CU 008/2011). Safety can be ensured by the requirement of tightness of the body of toys, as well as a significant reduction in the service life of such products.

10.47470/0016-9900-2022-101-5-539-544

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NOVEL BIOMARKERS OF INVASIVE ASPERGILLOSIS CAUSATIVE AGENTS FROM SPECTRA-FORMING MOLECULES REVEALING BY LINEAR MALDI-TOF MASS-SPECTOMETRY

Igor A. Ryabinin N. V. Vasilyeva

10.36233/978-5-9900432-9-9-223

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Effects of Noopept and Piracetam on Depression of the Acetylcholine-Induced Current in Common Snail Command Neurons

Neuroscience and Behavioral Physiology (2020)

А. S. Pivovarov Г. Б. Мурзина N. V. Vasilyeva

Piracetam

Dephosphorylation

10.1007/s11055-020-01000-2

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Ultrastructural Patterns of Interactions between Murine Lung Macrophages and Yeast Cells of <I>Cryptococcus neoformans</I> Strains with Different Virulence

Medical Mycology Journal (2018)

Amaliya Stepanova N. V. Vasilyeva Masashi Yamaguchi Hiroji Chibana И А Босак

This article presents the ultrastructural patterns of interactions between the murine lung macrophages and cells of low- (RKPGY-881, -1165, -1178) and high-virulence (RKPGY-1090, -1095, -1106) strains of Cryptococcus neoformans at the seventh post-experimental day. It was found that if macrophages ingest living yeast cells, the latter can: 1) become completely free from polysaccharide capsules, after that their contents undergo lysis, and cell wall debris are extruded from the macrophage (first scenario); 2) become partly free from their capsules, destroy the phagosomal plasma membrane and induce destructive processes inside the macrophage causing their death (second scenario); or 3) not lose their capsules and localize inside macrophage in latent state (third scenario). Macrophages can also ingest senescent and dead C. neoformans cells surrounded by capsules that are lost at the ingesting and phagosome stages (fourth scenario). The study revealed the dependence of cell-mediated immunity on the stage of development of ingested C. neoformans yeast cells. Here we describe a new mechanism of capsular polysaccharide elimination of C. neoformans yeast cells by murine macrophages.

10.3314/mmj.16-00009

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Citations (1)

State of immune status in hematological patients with invasive aspergillosis

DOAJ (DOAJ: Directory of Open Access Journals) (2014)

Е. В. Фролова О. В. Шадривова Л. В. Филиппова А Е Учеваткина А. Г. Волкова

Immune status

10.22625/2072-6732-2012-4-4-59-64

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Species boundaries in the Trichophyton mentagrophytes / T. interdigitale species complex

Medical Mycology (2018)

Ivan M. Pchelin Daniil V. Azarov Maria A Churina Sergey G. Scherbak Svetlana V. Apalko

The fungi Trichophyton mentagrophytes and T. interdigitale are closely related species, causing superficial infections in humans and other mammals. The status of these taxa is a field of long-lasting debates. To clarify their phylogenetic relationships within the genus Trichophyton and sharpen the species boundaries, we performed sequencing of four T. mentagrophytes genomes and also evaluated three previously published multilocus data sets. We performed computational species delimitation analysis on all available in GenBank internal transcribed spacer region (ITS) sequences of Trichophyton spp. Phylogenomic data, phylogenetic network, and species delimitation analyses implied that T. mentagrophytes and T. interdigitale belong to the same phylogenetic species. However, we argue that taxonomic status quo should be retained, from the perspective of epidemiological data and the principle of taxonomic stability. Since there is a correlation between ITS genotype and epidemiological source of an isolate, restriction of T. interdigitale to purely anthropophilic ITS genotypes seems to be reasonable.

10.1093/mmy/myy115

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Citations (32)

Mutational analysis of the DJ-1 gene in sporadic patients with amyotrophic lateral sclerosis

V Viviani Massimo Cogliati Maria Carmela Esposto Kimberly C. Lemmer Kathrin Tintelnot

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