Parinita Agrawal

National Institute of Technology Rourkela

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Krishna Pramanik

National Institute of Technology Rourkela

Arun Chandru

Biotech Park

Tuhin Bhowmick

Indian Institute of Science Bangalore

Veena Sangwan

Dr. Shroff’s Charity Eye Hospital

Kamalnath Selvakumar

Biotech Park

Suvro Kanti Chowdhury

Biotech Park

Utkarsh Bhutani

Indian Institute of Technology Hyderabad

Amit Biswas

National Institute of Technology Rourkela

Neha Waghmare

Biotech Park

Abha Gour

Dr. Shroff’s Charity Eye Hospital

Cooperative Institutions

Biotech Park

Dr. Shroff’s Charity Eye Hospital

National Institute of Technology Rourkela

L V Prasad Eye Institute

Creative Commons

Christie's

All India Institute of Medical Sciences

All India Institute of Medical Sciences Raipur

Post Graduate Institute of Medical Education and Research

Singapore Eye Research Institute

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Human Cadaveric Donor Cornea Derived Extra Cellular Matrix Microparticles for Minimally Invasive Healing/Regeneration of Corneal Wounds

Biomolecules (2021)

Arun Chandru Parinita Agrawal Sanjay Kumar Ojha Kamalnath Selvakumar Vaishnavi K. Shiva

Biological materials derived from extracellular matrix (ECM) proteins have garnered interest as their composition is very similar to that of native tissue. Herein, we report the use of human cornea derived decellularized ECM (dECM) microparticles dispersed in human fibrin sealant as an accessible therapeutic alternative for corneal anterior stromal reconstruction. dECM microparticles had good particle size distribution (≤10 µm) and retained the majority of corneal ECM components found in native tissue. Fibrin–dECM hydrogels exhibited compressive modulus of 70.83 ± 9.17 kPa matching that of native tissue, maximum burst pressure of 34.3 ± 3.7 kPa, and demonstrated a short crosslinking time of ~17 min. The fibrin–dECM hydrogels were found to be biodegradable, cytocompatible, non-mutagenic, non-sensitive, non-irritant, and supported the growth and maintained the phenotype of encapsulated human corneal stem cells (hCSCs) in vitro. In a rabbit model of anterior lamellar keratectomy, fibrin–dECM bio-adhesives promoted corneal re-epithelialization within 14 days, induced stromal tissue repair, and displayed integration with corneal tissues in vivo. Overall, our results suggest that the incorporation of cornea tissue-derived ECM microparticles in fibrin hydrogels is non-toxic, safe, and shows tremendous promise as a minimally invasive therapeutic approach for the treatment of superficial corneal epithelial wounds and anterior stromal injuries.

Decellularization

10.3390/biom11040532

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Citations (21)

Novel Blowspun Nanobioactive Glass Doped Polycaprolactone/Silk Fibroin Composite Nanofibrous Scaffold with Enhanced Osteogenic Property for Bone Tissue Engineering

Fibers and Polymers (2018)

Parinita Agrawal Krishna Pramanik Akalabya Bissoyi

Fibroin

Polycaprolactone

RUNX2

10.1007/s12221-018-8601-5

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Citations (7)

Fabrication of chitosan-based nanofibrous scaffold using free surface electrospinning for tissue engineering application

Asian Journal of Tropical Biotechnology (2017)

Parinita Agrawal Krishna Pramanik

Abstract. Agrawal P, Pramanik K. 2017. Fabrication of chitosan-based nanofibrous scaffold using free surface electrospinning for tissue engineering application. Bioteknologi 14: 60-70. Tissue engineering offers a promising approach for repair of defective tissues and organs. Developing scaffold from a variety of polymer blends or composites allows the adjustment of the properties aimed at duplicating required tissue. In recent years, considerable attention has been given to develop chitosan-based biomaterials for their applications in the field of tissue engineering due to its minimal foreign body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures that are suitable for cell ingrowth and osteoconduction. The present work involves the preparation of nanofibrous mat from chitosan blended with other biopolymers such as silk fibroin, poly-vinyl alcohol, and polyethylene oxide by free surface electrospinning method. The morphology and functional characterization of the developed scaffolds was performed by SEM and FTIR studies. The average fiber diameter of 269 nm and 122 nm were obtained with chitosan/polyvinyl alcohol and chitosan/silk fibroin, poly ethylene oxide blends respectively. Crystalline nature of the scaffolds was confirmed by XRD studies. The scaffolds are also shown to have desired biodegradable and biocompatible properties. Chitosan-based polymeric scaffolds are thus proved to be potential materials for tissue engineering applications.

Electrospinning

10.13057/biotek/c140205

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Biopolymeric corneal lenticules by digital light processing based bioprinting: a dynamic substitute for corneal transplant

Biomedical Materials (2024)

Utkarsh Bhutani Namit Dey Suvro Kanti Chowdhury Neha Waghmare Rita Das Mahapatra

Digital light processing (DLP) technology has gained significant attention for its ability to construct intricate structures for various applications in tissue modeling and regeneration. In this study, we aimed to design corneal lenticules using DLP bioprinting technology, utilizing dual network bioinks to mimic the characteristics of the human cornea. The bioink was prepared using methacrylated hyaluronic acid and methacrylated gelatin, where ruthenium salt and sodium persulfate were included for mediating photo-crosslinking while tartrazine was used as a photoabsorber. The bioprinted lenticules were optically transparent (85.45% ± 0.14%), exhibited adhesive strength (58.67 ± 17.5 kPa), and compressive modulus (535.42 ± 29.05 kPa) sufficient for supporting corneal tissue integration and regeneration. Puncture resistance tests and drag force analysis further confirmed the excellent mechanical performance of the lenticules enabling their application as potential corneal implants. Additionally, the lenticules demonstrated outstanding support for re-epithelialization and stromal regeneration when assessed with human corneal stromal cells. We generated implant ready corneal lenticules while optimizing bioink and bioprinting parameters, providing valuable solution for individuals suffering from various corneal defects and waiting for corneal transplants.

3D bioprinting

Corneal Transplant

10.1088/1748-605x/ad3312

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Citations (2)

Chitosan-poly(vinyl alcohol) nanofibers by free surface electrospinning for tissue engineering applications

Tissue Engineering and Regenerative Medicine (2016)

Parinita Agrawal Krishna Pramanik

Vinyl alcohol

Biocompatibility

Electrospinning

10.1007/s13770-016-9092-3

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Citations (76)

Kuragel: A biomimetic hydrogel scaffold designed to promote corneal regeneration

iScience (2024)

Parinita Agrawal Anil Tiwari Suvro Kanti Chowdhury Mehak Vohra Abha Gour

Cornea-related injuries are the most common cause of blindness worldwide. Transplantation remains the primary approach for addressing corneal blindness, though the demand for donor corneas outmatches the supply by millions. Tissue adhesives employed to seal corneal wounds have shown inefficient healing and incomplete vision restoration. We have developed a biodegradable hydrogel - Kuragel, with the ability to promote corneal regeneration. Functionalized gelatin and hyaluronic acid form photo-crosslinkable hydrogel with transparency and compressive modulus similar to healthy human cornea. Kuragel composition was tuned to achieve sufficient adhesive strength for sutureless integration to host tissue, with minimal swelling post-administration. Studies in the New Zealand rabbit mechanical injury model affecting corneal epithelium and stroma demonstrate that Kuragel efficiently promotes re-epithelialization within 1 month of administration, while stroma and sub-basal nerve plexus regenerate within 3 months. We propose Kuragel as a regenerative treatment for patients suffering from corneal defects including thinning, by restoration of transparency and thickness.

10.1016/j.isci.2024.109641

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Citations (4)

Enhanced chondrogenesis of mesenchymal stem cells over silk fibroin/chitosan‐chondroitin sulfate three dimensional scaffold in dynamic culture condition

Journal of Biomedical Materials Research Part B Applied Biomaterials (2018)

Parinita Agrawal Krishna Pramanik Varshini Vishwanath Amit Biswas Akalabya Bissoyi

Abstract Chondroitin sulfate (Ch) is one of the main structural components of cartilage tissue, therefore, its presence in tissue engineered scaffold is expected to enhance cartilage regeneration. Previously, silk fibroin/chitosan (SF/CS) blend was proven to be a potential biomaterial for tissue development. In this study, the effect of Ch on physicochemical and biological properties of SF/CS blend was investigated and scaffolds with 0.8 wt% Ch was found to be favorable. The scaffolds possess pore size of 37–212 µm, contact angle 46.2–50.3°, showed controlled swelling and biodegradation. The biocompatibility of scaffold was confirmed by subcutaneous implantation in mouse. Human mesenchymal stem cells (hMSCs) seeded scaffolds cultured under spinner flask bioreactor promoted cell attachment, proliferation, distribution, and metabolic activity in vitro . The histology and immunofluorescence studies revealed that combined effect of Ch and dynamic condition resulted in higher glycosaminoglycan secretion and native cartilage type matrix synthesis in comparison to SF/CS scaffolds used as control. Higher expression of collagen‐II, Sox9, aggrecan and decrease in collagen‐I expression represented by quantitative polymerase chain reaction study confirmed the progression of chondrogenic differentiation. This study successfully demonstrates the potentiality of SF/CS‐Ch scaffold for hMSCs recruitment and redirecting cartilage tissue regeneration with enhanced chondrogenesis. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2576–2587, 2018.

Chondrogenesis

Fibroin

Aggrecan

Biomaterial

10.1002/jbm.b.34074

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Citations (25)

Chondrogenic Differentiation of Mesenchymal Stem Cells on Silk Fibroin:chitosan–Glucosamine Scaffold in Dynamic Culture

Regenerative Medicine (2018)

Parinita Agrawal Krishna Pramanik Amit Biswas

Aim: Cartilage damage is a common age-related problem that leads to progressive proteoglycan loss. Glucosamine stimulates proteoglycan synthesis and, therefore, its effect on the cartilage extracellular matrix synthesis over silk fibroin:chitosan (SF:CS) tissue-engineered scaffold was investigated for cartilage construct generation. Materials & methods: Human mesenchymal stem cells (hMSCs) were cultured and differentiated over SF:CS–glucosamine porous scaffold, under dynamic culture condition in spinner flask bioreactor. Results: hMSCs-seeded scaffold in dynamic culture exhibited homogenous cell distribution, proliferation and higher cell density at the core than static culture. Glucosamine in scaffold promoted proteoglycan and collagenous matrix synthesis as revealed by histological and immunofluorescence studies. Quantitative-PCR analysis showed upregulation of cartilage-specific genes, thereby confirming the chondrogenic differentiation. Conclusion: The chondrogenic differentiation of hMSCs was enhanced by the synergistic effect of glucosamine incorporated in SF:CS scaffold and influence of 3D dynamic culture environment, thereby resulting in chondrogenic phenotype of the cells that promoted cartilage regeneration.

Fibroin

Chondrogenesis

10.2217/rme-2017-0159

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Citations (21)

Enhanced chondrogenic differentiation of human mesenchymal stem cells in silk fibroin/chitosan/glycosaminoglycan scaffolds under dynamic culture condition

Differentiation (2019)

Parinita Agrawal Krishna Pramanik

Fibroin

Chondrogenesis

Aggrecan

Matrix (chemical analysis)