T. E. Koshkina

All-Russian Scientific Research Institute of Food Biotechnology

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СК

С. К. Завриев

Institute of Bioorganic Chemistry

N I Lukhovitskaia

I. V. Shmyglya

Potato Research Institute

Elena A. Usacheva

NorthShore University HealthSystem

S. K. Zavriev

All-Russian Scientific Research Institute of Food Biotechnology

B.A. Biryukova

СН

С. Н. Кирсанова

Potato Research Institute

OА

O. А. Shulga

Lesya Ukrainka Volyn National University

МП

М. П. Бекетова

All-Russian Scientific Research Institute of Food Biotechnology

СМ

С.Ю. Морозов

Lomonosov Moscow State University

Cooperative Institutions

All-Russian Scientific Research Institute of Food Biotechnology

Russian Academy of Sciences

Lomonosov Moscow State University

Federal Agency for Scientific Organizations

Potato Research Institute

Russian State Agrarian University - Moscow Timiryazev Agricultural Academy

Institute of Bioorganic Chemistry

Russian Academy of Natural Sciences

Moscow State University

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Interaction of the Carlavirus Cysteine-Rich Protein with the Plant Defense System

Molecular Biology (2005)

Nina I. Lukhovitskaya Andrey G. Solovyev T. E. Koshkina С. К. Завриев Sergey Y. Morozov

Potato virus X

Agroinfiltration

10.1007/s11008-005-0094-9

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Citations (10)

[Interaction of cysteine-rich protein of Carlavirus with plant defense system].

N I Lukhovitskaia А. Г. Соловьева T. E. Koshkina С. К. Завриев С.Ю. Морозов

Viruses of genus Carlavirus encode a small cysteine-rich protein (CRP) of unknown function. To investigate the role of CRP of carlavirus chrysanthemum virus B (CVB), a recombinant potato virus X (PVX) genome was constructed, which carried the CVB CRP gene. Expression of CVB CRP in the PVX genetic background drastically changed the PVX symptom phenotype in N. benthamiana. Instead of symptomless infection and mild mosaic, which are characteristic of PVX in this plant host, the recombinant virus expressing CVB CRP induced formation of necrotic local lesions on inoculated leaves and necrosis of the apical leaves. In N. tabacum, the infection pattern depended on the host genotype: the recombinant PVX was able to spread systemically only in N gene-carrying plants. In agroinfiltration-mediated transient expression assay, CVB CRP did not exhibit the properties of avirulence factor in N. benthamiana and was unable to suppress post-transcriptional gene silencing. Thus, CVB CRP is the viral pathogenicity determinant controlling the virus interaction with plant hosts in a manner which depends on plant defense mediated by resistance genes such as the N gene.

Potato virus X

Agroinfiltration

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Effect of cocksfoot mottle virus defective RNA on accumulation of virus capsid protein in wheat plants

Russian Agricultural Sciences (2007)

N. V. Kokorev T. E. Koshkina D. Yu. Ryazantsev С. К. Завриев

Subgenomic mRNA

Brome mosaic virus

RNA virus

Mottle

Tobamovirus

10.3103/s1068367407030056

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Citations (3)

[A point mutation in the coat protein gene affects long distance transport of the tobacco mosaic virus].

Molecular Biology (2003)

T. E. Koshkina Ekaterina N. Baranova Elena A. Usacheva S. K. Zavriev

Wild type

10.1023/a:1025155931497

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Generation of potato transgenic lines resistant to potato leafroll virus and potato spindle tuber viroid

Lyudmila Khromova М. П. Бекетова I. V. Shmyglya С. Н. Кирсанова B.A. Biryukova

Resistance of transgenic potato lines of the cultivar Zhukovskiy ranniy to Potato Leafroll Virus (PLRV) and to Potato Spindle Tuber Viroid (PSTV) was studied. Genetic construct used for plant transformation carries an expression cassette of hybrid RNA containing the antisense RNA fragment of PLRV replicase (852-990a. a.) and the fragment of PSTV RNA (58-214n.). One from the four generated transgenic lines has demonstrated resistance to both pathogens in laboratory tests with in vitro plants. Leaves of transgenic potato plants were mechanically inoculated by sap from a viroid-infected plant. Infection development was tested by RT-PCR. Transgenic potato plants were inoculated with purified PLRV through cut roots. PLRV accumulation in plants was detected by ELISA of samples from the lateral shoot of the virus-infected plants.

Potato leafroll virus

10.3920/9789086866083_042

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