Guneet Kumar

SuGanit Systems (United States)

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Gregory F. Payne

University of Maryland, College Park

Paul J. Smith

Neurobehavioral Systems

Michael J. Cooney

University of Hawaiʻi at Mānoa

Tianhong Chen

Zhongnan Hospital of Wuhan University

Godwin Severa

University of Hawaiʻi at Mānoa

Marilyn K. Speedie

University of Minnesota

Linda A. Geelhaar

University of Maryland, Baltimore

Michael T. Harris

Purdue University West Lafayette

Min Meng

Liaocheng People's Hospital

J. R. Stacy

Naval Surface Warfare Center

Cooperative Institutions

University of Maryland, College Park

University of Maryland, Baltimore County

Daiichi Sankyo (India)

Virginia Tech

University of Maryland, Baltimore

United States Food and Drug Administration

University of Hawaii System

University of Hawaiʻi at Mānoa

College of Industrial Technology

Nihon University

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Corecovery of lipids and fermentable sugars from Rhodosporidium toruloides using ionic liquid cosolvents: Application of recycle to batch fermentation

Biotechnology Progress (2014)

Godwin Severa Guneet Kumar Michael J. Cooney

This work evaluates the ability of an ionic liquid‐methanol cosolvent system to extract lipids and recycle fermentable sugars recovered from oil‐bearing Rhodosporidium toruloides grown in batch culture on defined media using glucose and xylose as carbon sources. Growth on the recycled mixed carbon substrate was successful with glucose consumed before xylose and overall cell mass to lipid yields (Y P/X ) between 57% and 61% (w/w relative to whole dried cell mass) achieved. Enzymatic hydrolysis of the delipified carbohydrate fraction recovered approximately 9%–11% (w/w) of the whole dried cell mass as fermentable sugars, which were successfully recycled as carbon sources without further purification. In total, up to 70% (w/w) of the whole dried cell mass was recovered as lipids and fermentable sugars and the substrate to lipid yields (Y P/S ) was increased from 0.12 to 0.16 g lipid/g carbohydrate consumed, highlighting the promise of this approach to process lipid bearing cell biomass. © 2014 American Institute of Chemical Engineers Biotechnol. Prog ., 30:1239–1242, 2014

10.1002/btpr.1952

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Citations (3)

Corecovery of Bio-Oil and Fermentable Sugars from Oil-Bearing Biomass

International Journal of Chemical Engineering (2013)

Godwin Severa Guneet Kumar Michael J. Cooney

The applicability of ionic liquid-methanol cosolvent system to both extract bio-oil and simultaneously pretreat the carbohydrate fraction of jatropha and safflower biomass for enzymatic hydrolysis to fermentable sugars is presented. Although pretreatment with either the cosolvent or pure ionic liquid yielded comparable hydrolysis kinetics and fermentable sugar yields on safflower whole seeds, the addition of alcohol to the ionic liquid was necessary to optimally recover both bio-oil and fermentable sugars. The ionic liquid [C2mim][Ac] was far more effective than [C2mim][MeSO 4 ] with optimum processing conditions occurring at a cosolvent concentration of 70–30 wt% of [C2mim][Ac] to methanol and a processing temperature of 120°C. Under these conditions, the majority of the bio-oil was extracted and 25.4 wt% (safflower) and 14.3 wt% ( jatropha ) of the whole seed biomass were recovered as fermentable sugars. The recovery of fermentable sugars from the carbohydrate fraction was as high as 74% and 78% for jatropha and safflower seeds, respectively, when using [C2mim][Ac] cosolvent. A preliminary theoretical analysis of two potential oil seed processing pathways using the cosolvent system suggested that the corecovery of bio-oil, fermentable sugars, and a protein rich meal can recover a majority of the energy contained in the original biomass—a result that improves upon the traditional approach of solely extracting bio-oil.

Fraction (chemistry)

Jatropha

Reducing sugar

10.1155/2013/617274

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Citations (20)

Simultaneous extraction and separation of phorbol esters and bio-oil from Jatropha biomass using ionic liquid–methanol co-solvents

Separation and Purification Technology (2013)

Godwin Severa Guneet Kumar Mike Troung Gregory Young Michael J. Cooney

Jatropha

Palm kernel

10.1016/j.seppur.2013.06.001

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Citations (33)

Plant cell biodegradation of a xenobiotic nitrate ester, nitroglycerin

Nature Biotechnology (1997)

Akshay Goel Guneet Kumar Gregory F. Payne Shyam K. Dube

Nitroglycerin (drug)

Xenobiotic

10.1038/nbt0297-174

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Citations (67)

Enzymatic modification of the synthetic polymer polyhydroxystyrene

Enzyme and Microbial Technology (1999)

Lianhe Shao Guneet Kumar Joseph L. Lenhart Paul J. Smith Gregory F. Payne

Michael reaction

10.1016/s0141-0229(99)00111-8

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Citations (60)

Enzymatic gelation of the natural polymer chitosan

Polymer (2000)

Guneet Kumar Joseph F. Bristow Paul J. Smith Gregory F. Payne

Chitosanase

Biopolymer

10.1016/s0032-3861(99)00360-2

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Citations (130)

Enzymatic grafting of hexyloxyphenol onto chitosan to alter surface and rheological properties

Biotechnology and Bioengineering (2000)

Tianhong Chen Guneet Kumar Michael T. Harris Paul J. Smith Gregory F. Payne

An enzymatic method to graft hexyloxyphenol onto the biopolymer chitosan was studied. The method employs tyrosinase to convert the phenol into a reactive o-quinone, which undergoes subsequent nonenzymatic reaction with chitosan. Reactions were conducted under heterogeneous conditions using chitosan films and also under homogeneous conditions using aqueous methanolic mixtures capable of dissolving both hexyloxyphenol and chitosan. Tyrosinase was shown to catalyze the oxidation of hexyloxyphenol in such aqueous methanolic solutions. Chemical evidence for covalent grafting onto chitosan was provided by three independent spectroscopic approaches. Specifically, enzymatic modification resulted in (1) the appearance of broad absorbance in the 350-nm region of the UV/vis spectra for chitosan films; (2) changes in the NH bending and stretching regions of chitosan's IR spectra; and (3) a base-soluble material with 1H-NMR signals characteristic of both chitosan and the alkyl groups of hexyloxyphenol. Hexyloxyphenol modification resulted in dramatic changes in chitosan's functional properties. On the basis of contact angle measurements, heterogeneous modification of a chitosan film yielded a hydrophobic surface. Homogeneously modified chitosan offered rheological properties characteristic of associating water-soluble polymers. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 564–573, 2000.

Biopolymer

Chemical modification

Surface Modification

10.1002/1097-0290(20001205)70:5<564::aid-bit11>3.0.co;2-w

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Citations (119)

Enzymatic grafting of hexyloxyphenol onto chitosan to alter surface and rheological properties

Biotechnology and Bioengineering (2000)

Tianhong Chen Guneet Kumar Michael T. Harris Paul J. Smith Gregory F. Payne

Surface Modification

10.1002/1097-0290(20001205)70:5<564::aid-bit11>3.3.co;2-n

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Citations (6)