M. S. Chauhan

National Dairy Research Institute

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S. K. Singla

National Dairy Research Institute

129

R. S. Manik

National Dairy Research Institute

106

P. Palta

National Dairy Research Institute

P. Palta

National Dairy Research Institute

Manoj Kumar Singh

National Dairy Research Institute

Naresh L. Selokar

National Dairy Research Institute

Aman George

National Eye Institute

M. K. Singh

Central Institute for Research on Goats

Monika Saini

Central Institute for Research on Buffaloes

Karn Pratap Singh

National Dairy Research Institute

Cooperative Institutions

National Dairy Research Institute

132

Indian Council of Agricultural Research

Central Institute for Research on Goats

Indian Veterinary Research Institute

All India Institute of Medical Sciences

National Bureau of Animal Genetic Resources

Assam Agricultural University

Banaras Hindu University

University of Delhi

Central Institute for Research on Buffaloes

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Reduced graphene oxide/NiO nanocomposites as a highly effective, efficient, and recyclable heterogeneous catalyst towards one pot of synthesis of 2-arylbenzoxazoles/thiazoles

Journal of Porous Materials (2025)

Sneha Naik Richa Tiwari Renu Verma M. S. Chauhan Narendra Pal Lamba

Non-blocking I/O

One-pot synthesis

10.1007/s10934-024-01733-2

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Citations (0)

Effect of Sex of Embryo on Developmental Competence, Epigenetic Status, and Gene Expression in Buffalo (Bubalus bubalis) Embryos Produced by Hand-Made Cloning

Cellular Reprogramming (2016)

Anjit Sandhu Sushil K. Mohapatra Himanshu Agrawal Manoj Kumar Singh P. Palta

Buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. Although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p < 0.05) whereas the apoptotic index (6.39 ± 0.25 vs. 8.52 ± 0.38) was lower (p < 0.05) for male than for female blastocysts. In the blastocysts, the global level of H3K18ac was found to be in the following order: male>female>IVF (in vitro fertilization) blastocysts (p < 0.05). The global level of H3K9me2 was not significantly different between male and female blastocysts and was higher (p < 0.05) compared with that in their IVF counterparts. The relative mRNA abundance of X-chromosome-linked (XIST, HPRT, PGK, and G6PD), apoptosis- (CASPASE3) and pregnancy-related genes (IFN-τ) was significantly higher (p < 0.05) whereas that of DNMT1 was significantly lower (p < 0.05) in female than in male blastocysts; however, in the case of apoptosis- (BCL-XL) and developmental competence-related genes (IGF1R and OCT4), the expression level was similar between the two groups. The gene expression level of OCT4 and IFN-τ but not of IGF1R was significantly lower (p < 0.05) in cloned than in IVF blastocysts. This study demonstrates that the epigenetic status, quality, and expression level of several genes but not the developmental competence are affected by the sex of cloned embryos.

Bubalus

10.1089/cell.2015.0077

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In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell

Journal of Assisted Reproduction and Genetics (2012)

Sushila Kala Ravinder Kaushik Surendra Singh Prashant Kadam Manoj Kumar Singh

Percoll

Homeobox protein NANOG

CD90

10.1007/s10815-012-9883-y

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Citations (40)

Calcium ionophore enhanced developmental competence and apoptotic dynamics of goat parthenogenetic embryos produced in vitro

In Vitro Cellular & Developmental Biology - Animal (2019)

Diksha Dua D. Nagoorvali M. S. Chauhan P. Palta Puniti Mathur

Parthenogenesis

Cleavage (geology)

10.1007/s11626-019-00322-x

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Citations (7)

Optimization of Serum-Free Culture Conditions for Propagation of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells

Cellular Reprogramming (2019)

Ankur Sharma Syed Mohmad Shah Neha Saini Parul Mehta B. S. Bharath Kumar

Spermatogonial stem cells (SSCs) self-renew and produce a large number of differentiated germ cells to maintain normal spermatogenesis. However, the growth factors crucial for SSC self-renewal and the mechanism underlying this process remain unclear. In the present study, a serum-free culture media was used to evaluate the effect of several growth factors on the expression of some SSC markers and self-renewal related genes. The putative SSCs were cultured on buffalo Sertoli cell feeder layer in KO-DMEM +10% KOSR. The colony formation was observed between 7 and 10 days. The putative SSC colonies also expressed markers specific for undifferentiated type A spermatogonia and pluripotency markers. After 15 days, relative mRNA expression study revealed that 20 ng/mL concentration of Glial cell line-derived neurotrophic factor (GDNF) upregulated the expression of PLZF, TAF4B, and THY1. Furthermore, supplementation of a combination of 20 ng/mL GDNF, 10 ng/mL basic fibroblast growth factor (bFGF), 1000 IU/mL leukemia inhibitory factor (LIF), and 1 ng/mL colony stimulating factor 1 (CSF1) upregulated the expression of PLZF, TAF4B, BCL6B, and ID4 genes. These results demonstrated that our defined culture media in combination with GDNF, bFGF, LIF, and CSF1 well supported SSC self-renewal.

10.1089/cell.2018.0018

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Citations (15)

Homologous transplantation of fluorescently labelled enriched buffalo (Bubalus bubalis) spermatogonial stem cells to prepubertal recipients

The Indian Journal of Animal Sciences (2020)

Ankur Sharma A. Kumaresan Narendra Nala Manish Tiwari Mayank Roshan

Spermatogonial stem cell transplantation provides a unique opportunity to study the biology of spermatogenesis and also offers an alternative approach for genetic modification in large animals. The present study aimed to extend this technique to the water buffalo. Spermatogonial stem cells (SSCs) were isolated from prepubertal buffalo testes (3-6 months of age) using two-step enzymatic digestion method and enriched by differential plating and Percoll density gradient centrifugation. The enriched SSCs expressed numerous spermatogonial transcriptional markers, viz. ID4, THY1, BCL6B, UCHL1, ETV5 and REX1 which confirmed their bonafide SSC identity. Subsequently, the enriched SSCs were labelled with a fluorescent dye PKH26 and transplanted into buffalo calves under ultrasound guidance. The recipient testes were recovered after 7-8 weeks by castration and their fluorescence microscopebased examination exhibited the persistence and localization of the fluorescent donor cells within the recipient seminiferous tubules. Further validation was done by the flow cytometric evaluation of PKH26 labeled donor cells among those isolated by two-step enzymatic digestion of recipient testicular parenchyma. In conclusion, we demonstrated the feasibility of SSC transplantation technique in the water buffalo.

Differential centrifugation

Percoll

Parenchyma

Bubalus

10.56093/ijans.v90i5.104608

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Citations (1)

Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT

Theriogenology (2019)

Parul Mehta Ravinder Kaushik Kiran Singh Ankur Sharma M. K. Singh

Bubalus

10.1016/j.theriogenology.2019.06.004

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Citations (11)

Effect of basic fibroblast growth factor (FGF2) on cumulus cell expansion, in vitro embryo production and gene expression in buffalo (Bubalus bubalis)

Reproductive Biology (2020)

Satish Kumar S. K. Singla Radheysham Manik P. Palta M. S. Chauhan

Bubalus

In vitro maturation

10.1016/j.repbio.2020.08.003

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Citations (9)

Reproductive biotechniques in buffaloes (Bubalus bubalis): status, prospects and challenges

Reproduction Fertility and Development (2009)

Birbal Singh M. S. Chauhan S. K. Singla Sanjeev K. Gautam Vinod Verma

The swamp buffalo holds tremendous potential in the livestock sector in Asian and Mediterranean countries. Current needs are the faster multiplication of superior genotypes and the conservation of endangered buffalo breeds. Recent advances in assisted reproductive technologies, including in vitro embryo production methodologies, offer enormous opportunities to not only improve productivity, but also to use buffaloes to produce novel products for applications to human health and nutrition. The use of molecular genomics will undoubtedly advance these technologies for their large-scale application and resolve the key problems currently associated with advanced reproductive techniques, such as animal cloning, stem cell technology and transgenesis. Preliminary success in the application of modern reproductive technologies warrants further research at the cellular and molecular levels before their commercial exploitation in buffalo breeding programmes.

Bubalus

10.1071/rd08172

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Citations (53)

Comparative metabolomics analysis of Halari donkey colostrum and mature milk throughout lactation stages using 1H Nuclear Magnetic Resonance

LWT (2023)

Renu Garhwal Karnam Sangwan Rahul Mehra Anuradha Bhardwaj Yash Pal

Since donkey milk has a similar composition to human milk, it has been well demonstrated that it is an excellent alternative. The content of donkey milk (DM) changes during lactation, nevertheless, has not been thoroughly researched. The overall objective was to explore the metabolite changes in donkey colostrum and mature milk using 1H Nuclear Magnetic Resonance (NMR) spectroscopy. In both colostrum and donkey mature milk included total 36 metabolites that were identified. The metabolites were differentially regulated (p < 0.05), where 24 showed upregulation and 28 were downregulated. 24 (14 upregulated, 10 downregulated), 10 (1 upregulated, 9 downregulated), and 18 (9 upregulated, 9 downregulated) differentially expressed metabolites were identified during lactation period of Colostrum-early lactation, early lactation - mid lactation, and mid lactation-late lactation, respectively. This proved that lactation altered the content of the donkey milk. To further understand the mechanisms that changed the milk during lactation, the interactions and metabolic pathways were examined in more detail. These studies provide thorough understandings of the metabolite changes that occur during lactation period which will offer useful guidance for further creating DM products with improved bioactivities, and it will help future studies into nutritional research and the isolation of useful components.

10.1016/j.lwt.2023.114805

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Citations (10)