Ali Moradi

Shahid Sadoughi University of Medical Sciences and Health Services

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Fatemeh Safari

Shahid Sadoughi University of Medical Sciences and Health Services

Mahdie Hemati

Shahid Sadoughi University of Medical Sciences and Health Services

MUHAMMAD Z ARIF

Ameneh Khodarahmi

Shahid Sadoughi University of Medical Sciences and Health Services

Alireza Karimollah

Shahid Sadoughi University of Medical Sciences and Health Services

Zohreh‐al‐sadat Ghoreshi

Kerman University of Medical Sciences

Saman Hosseinkhani

Tarbiat Modares University

Fateme Haghiralsadat

Shahid Sadoughi University of Medical Sciences and Health Services

Sattar Ghader

Shahid Bahonar University of Kerman

Najmeh Sadat Hosseini Motlagh

Mofid University

Cooperative Institutions

Shahid Sadoughi University of Medical Sciences and Health Services

108

Tehran University of Medical Sciences

University of Tehran

Tarbiat Modares University

Shahid Bahonar University of Kerman

Shiraz University of Medical Sciences

Mashhad University of Medical Sciences

Islamic Azad University, Tehran

Yazd University

Biotechnology Research Center

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The effect of melittin on the inhibition of Rac1 expression in HeLa cervical cancer cell lines

KAUMS Journal (2015)

Neda Eivazi-Arvanagh Arezo Chakerzehi Mahdieh Hemati Hananeh Zarinnehad Ali Moradi

Background: Cell migration plays a principal role in many biological and pathological processes, including embryonic and tumor metastasis. It is well-known that Rac1 from Rho GTPase family is the key regulator of the cell migration. Melittin is a natural peptide in bee venom with apoptotic and anti cancer effects. This study aimed to examine the effect of melittin on Rac1 expression. Materials and Methods: HeLa cells were cultured in Dulbecco's modified Eagle's medium until they reached 80% confluency, then treated with melittin (0.5 and 1 μg/ml) with the consideration of IC50=1 μg/ml for 6 hours. In the control group the same volume of distilled water, as the melittin solvent, was added. Thereafter, cells were lyzed with buffer and after centrifugation the protein concentration of supernatant was measured using the Bradford method. The amount of Rac1 expression was determined using the Western blotting technique and chemiluminescence. Outcomes were normalized with β-Actin as an internal control and the results were displayed as the percentage against the control. Results: The Rac1expression compared to the control (100%), in the presence of different concentrations of melittin (0.5 and 1 μg/ml) were 13.88%±1.15% and 1.7%±0.96%, respectively which showed a significant reduction in Rac1 melittin expression (P<0.05). Conclusion: It can be concluded that melittin can dose-dependently inhibit the Rac1 expression in HeLa cell line.

Melittin

HeLa

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Inhibition of GTPase Rac1 expression by vitamin D mitigates pressure overload-induced cardiac hypertrophy

IJC Heart & Vasculature (2021)

Ali Moradi Abdulbaset Maroofi Mahdie Hemati Tahmine Hashemzade Nasrin Alborzi

1, 25-dihydroxy Vitamin D3 (VitD) attenuates left ventricular hypertrophy (LVH), but the mechanisms remain to be portrayed in-depth. The small GTPase Rac1 plays a pivotal role in cardiovascular pathology, especially LVH. Here, we tested whether VitD exerts its anti-LVH effects by counteracting the small GTPase Rac1 expression.In Wistar rats, pressure overload-induced LVH was created by abdominal aortic banding. The animals were intraperitoneally administered VitD (0.1 µg/kg/d). Blood pressure was measured via carotid artery cannulation. Real-time RT-PCR and Western blotting were performed to assess the mRNA and protein expression, respectively. Myocardium was stained for determination of cardiomyocytes area and fibrosis. Lipid peroxidation levels and the activities of antioxidant enzymes were measured in left ventricular tissue.VitD significantly reduced hypertrophy markers (blood pressure, heart-to-body weight ratio, cardiomyocytes area, fibrosis as well as atrial and brain natriuretic peptides mRNA levels) compared to untreated groups (P < 0.05). VitD also improved the activity of antioxidant enzymes and reduced lipid peroxidation levels in the myocardium (P < 0.05). LVH hearts of untreated animals displayed a significant increase in Rac1 expression compared with the control group (P < 0.05). In contrast, cardiac Rac1, at either mRNAs or protein levels, was markedly lower in LVH animals receiving VitD compared with their untreated counterparts (P < 0.05).Our findings attest that VitD mitigates hallmarks of LVH imparted by pressure overload. Notably, VitD appears to perform its anti-LVH function partly through small GTPase Rac1 suppression. This, in turn, provides a robust incentive to consider VitD before LVH culminates in HF devastating disease.

Pressure overload

10.1016/j.ijcha.2021.100922

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Citations (5)

Production of Chlorosulfonated Rubber from Postconsumer Polyethylene and Evaluation of Produced Rubber Properties

Polymer-Plastics Technology and Engineering (2008)

Ali Moradi Ahmad Ramazani Mohammad Shahrokhi

In order to convert the postconsumer polyethylene to a valuable product, chlorosulfonation of virgin and highly degraded polyethylene has been carried out in the solution phase under atmospheric pressure. Produced chlorosulfonated polyethylene samples from virgin and degraded HDPE are compounded and cured at different temperatures and their mechanical properties are determined. The results show that degradation of used polyethylene does not have noticeable negative effects on the final mechanical properties of produced chlorosulfonated rubbers. Therefore, it can be concluded that chlorosulfonation can be considered as an effective new method for polyolefin recycling.

Polyolefin

High-density polyethylene

10.1080/03602550801977935

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Citations (1)

Effect of Thymol on Serum Antioxidant Capacity of Rats Following Myocardial Hypertrophy

Journal of Arak University of Medical Sciences (2017)

Mohabbat Jamhiri Zeinab Hafizibarjin Mojtaba Ghobadi Ali Moradi Fatemeh Safari

Thymol

Myocardial hypertrophy

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Citations (3)

Isolation of Melittin from Iranian Honey Bee Venom and Investigation of Its Effect on Proliferation of Cervical Cancer- HeLa Cell Line

DOAJ (DOAJ: Directory of Open Access Journals) (2013)

Kamran Pooshang Bagheri Amir Mahmoodzadeh H Zarinnahad Mehdi Mahdavi Delavar Shahbazzadeh

Melittin

HeLa

Bee Venom

Isolation

Cancer cell lines

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Citations (1)

Effect of Charge Distribution in a Flexible Loop on the Bioluminescence Color of Firefly Luciferases

Biochemistry (2009)

Ali Moradi Saman Hosseinkhani Hossein Naderi‐Manesh Majid Sadeghizadeh Bagher Said Alipour

Firefly luciferase is a monooxygenase that catalyzes the ATP-dependent conversion of firefly luciferin into a luciferyl-adenylate, which is oxidized to an electronically excited oxyluciferin in a multistep reaction and produces visible light with a remarkable quantum yield. The bioluminescence color of firefly luciferases is determined by the luciferase structure and assay conditions. Among different beetles, only luciferase from Phrixotrix railroad worm (PhRE) emits red bioluminescence, naturally. The presence of Arg353 in PhRE luciferase, which corresponds to the deleted residue in the other luciferases, is an important distinctive structural feature of it. Insertion of Arg356 into a green-emitter luciferase (Lampyris turkestanicus), corresponding to Arg353 in Phrixotrix hirtus, changed the emitted light from green to red. To further clarify the effect of this position on the light shift mechanism, four residues with similar sizes but different charges (Arg, Lys, Glu, and Gln) were inserted into Photinus pyralis luciferase, using site-specific insertion mutagenesis. Insertion of a residue with a positive side chain (Arg356 and Lys356) changed the light color to red, while insertion of a residue with a negative side chain (Glu356) had little effect on color. Insertion of a neutral residue (Gln356) at this position was performed without any change in bioluminescence spectra. Insertion of positively charged residues in this loop took place with a series of structural changes which were confirmed by fluorescence spectroscopy and homology modeling. Homology modeling reveals the appearance of a bulge in a flexible loop (T352−P359) upon mutation which shifts to the left side with a color change from green to red.

Luciferases

10.1021/bi802057w

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Citations (68)

Ear Acupuncture in Treating Trismus-Syndrome: A Case Report

Annals of Medical and Health Sciences Research (2017)

Bijan Pirnia Hamid Reza Taheri Nakhost Kambiz Pirnia Ali Moradi Maryam Teimouri

Trismus is a disease that is associated by muscle spasms in the jaw muscles, pain, and inability to open the mouth. Trismus is a Sympathomimetic effect of chronic use of methamphetamine. This study was carried out aimed to evaluate the efficacy of ear acupuncture on the pain and mouth opening in chronic methamphetamine-dependent patient. We report a case of 31-yearold young man with Trismus syndrome consequence of chronic use of methamphetamine with severe mouth pain. We applied ear acupuncture for three weeks to ameliorate pain and hopefully improve his functional ability to Mouth opening. Results were analyzed by generalized estimating equations (GEE) models. The primary outcome showed that Ear Acupuncture has not a significant effect on reducing the frequency of urine test during treatment. Also secondary outcome showed that there was not a significant reduction in pain index and mouth opening. According to the failure in the production of tyrosine Hydroxylase as a precursor to produce dopamine in chronic methamphetamine users and the role of dopaminergic system in the modulation of orals facial pain, the effectiveness of acupuncture could be in jeopardy.

Trismus

Gee

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Citations (2)

Molecular characterization of aquaglyceroporine: a novel mutation in LMAQP1 from LEISHMANIA MAJOR (MRHO/IR/75/ER)

11-ая Международная конференция по биоинформатике регуляции и структуры геномов и системной биологии (2018)

Gilda Eslami Maryam Ghavami Ali Moradi Hamid Nadri Salman Ahmadian

The first line treatment for cutaneous leishmaniasis is pentavalent antimony such as sodium stibogluconate (pentostam) and meglumine antimonite (glucantime). One of the most important ways to uptake the drug is by a trans-membrane protein, called aquaglyceroporin encoded by Aquaglyceroprotein1 (LmAQP1). In this study, molecular characterization of LmAQP1 was reported.Leishmania major (MRHO/IR/75/ER) promastigotes were cultured, and then DNA extraction and RNA extraction were done and followed by cDNA synthesis. Amplicons resulted from PCR and RT-PCR using specific primers were purified and sequenced. Molecular characterization was done by bioinformatically software such as BLST, ClustalW2, and RMSD.Amplicons resulted from PCR and RT-PCR showed equal size in length. BLASTn analysis showed a point nucleotide change in LmAQP1 gene that encoded 282-amino-acid long protein with a mutation at position 154 including replacement of alanine by threonine. The observed mutation in the interested gene was assessed using the above-mentioned software. The mentioned gene was submitted at GenBank, NCBI with accession number of KU514052.The functional prediction of the protein encoded from LmAQP1 showed that the mentioned mutation could not affect the three-dimension structure, but it may modify the drug uptake potential of this important channel. Based on from LmAQP1 role, it seems to be an appropriate candidate for drug development. According to search through internet, this is the first report of LmAQP1 from L. major (MRHO/IR/75/ER).

Amplicon

10.18699/bgrssb-2018-231

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Citations (2)

TAKAYASU ARTERITIS IN A YOUNG HISPANIC MALE

CHEST Journal (2024)

Ali Moradi AUSTIN SAUGSTAD BRETT DINNER KEVIN DANG ABILASH MURALIDHARAN

Takayasu Arteritis

Takayasu's arteritis

10.1016/j.chest.2024.06.3615

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A guide to properly select the defocusing distance for accurate solution of transport of intensity equation while testing aspheric surfaces

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2016)

Peyman Soltani Ahmad Darudi Ali Moradi Javad Amiri Georges Nehmetallah

In this paper, the Transport of Intensity Equation (TIE) for testing of an aspheric surface is verified experimentally. Using simulation, a proper defocus distance Δ𝑧 that leads to an accurate solution of TIE is estimated whenever the conic constant and configuration of the experiment are known. To verify this procedure a non-nulled experiment for testing an aspheric is used. For verification of the solution, the results are compared with the Shack-Hartmann sensor. The theoretical method and experimental results are compared to validate the results.

Conic section

Intensity

Constant (computer programming)

10.1117/12.2223208

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Citations (2)