Monika Niewiadomska

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

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A. Janik

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Joanna S. Kruszewska

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Urszula Perlińska-Lenart

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Grażyna Palamarczyk

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

U Lenart

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Jacek Lenart

Polish Academy of Sciences

Mateusz Juchimiuk

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Damian Kołakowski

Celon Pharma (Poland)

Grazyna B. Palamarczyk

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

M Wesierska

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Cooperative Institutions

Institute of Biochemistry and Biophysics, Polish Academy of Sciences

Polish Academy of Sciences

Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

WSB University

Medical University of Silesia

Zakład Doświadczalny Instytutu Zootechniki

National Research Institute of Animal Production

Szpital Uniwersytecki w Krakowie

Jagiellonian University

Long Beach Animal Hospital

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Spatial memory formation differentially affects c-Fos expression in retrosplenial areas during place avoidance training in rats

Acta Neurobiologiae Experimentalis (2016)

Monika Malinowska Monika Niewiadomska M Wesierska

The retrosplenial cortex is involved in spatial memory function, but the contribution of its individual areas is not well known. To elucidate the involvement of retrosplenial cortical areas 29c and 30 in spatial memory, we analyzed the expression of c-Fos in these areas in the experimental group of rats that were trained in a spatial place avoidance task, i.e. to avoid shocks presented in an unmarked sector of a stable arena under light conditions. Control rats were trained in the same context as the experimental rats either without (Control-noUS) or with shocks (Control-US) that were delivered in a random, noncontingent manner for three days. On the first day of place avoidance learning, the experimental group exhibited c-Fos induction in area 29c, similar to both control groups. In area 30, similarly high levels of c-Fos expression were observed in the experimental and Control-US groups. On the third day of training, when the experimental group efficiently avoided c-Fos expression in areas 29c and 30 was lower compared with the first day of training. In area 29c c-Fos level was also lower in the experimental than in comparison to the Control-US group. In area 30, c-Fos expression in the experimental group was lower than in both control groups. In conclusion, areas 29c and 30 appear to be activated during spatial memory acquisition on the first day of training, whereas area 30 seems suppressed during long-term memory functioning on the third day of training when rats effectively avoid.

Retrosplenial cortex

c-Fos

10.21307/ane-2017-024

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Citations (10)

The role of Alg13 N-acetylglucosaminyl transferase in the expression of pathogenic features of Candida albicans

Biochimica et Biophysica Acta (BBA) - General Subjects (2017)

Monika Niewiadomska A. Janik Urszula Perlińska-Lenart Sebastian Piłsyk Grażyna Palamarczyk

Transferase

10.1016/j.bbagen.2017.01.019

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Inhibition of Dephosphorylation of Dolichyl Diphosphate Alters the Synthesis of Dolichol and Hinders Protein N-Glycosylation and Morphological Transitions in Candida albicans

International Journal of Molecular Sciences (2019)

A. Janik Monika Niewiadomska Urszula Perlińska-Lenart Jacek Lenart Damian Kołakowski

The essential role of dolichyl phosphate (DolP) as a carbohydrate carrier during protein N-glycosylation is well established. The cellular pool of DolP is derived from de novo synthesis in the dolichol branch of the mevalonate pathway and from recycling of DolPP after each cycle of N-glycosylation, when the oligosaccharide is transferred from the lipid carrier to the protein and DolPP is released and then dephosphorylated. In Saccharomyces cerevisiae, the dephosphorylation of DolPP is known to be catalyzed by the Cwh8p protein. To establish the role of the Cwh8p orthologue in another distantly related yeast species, Candida albicans, we studied its mutant devoid of the CaCWH8 gene. A double Cacwh8∆/Cacwh8∆ strain was constructed by the URA-blaster method. As in S. cerevisiae, the mutant was impaired in DolPP recycling. This defect, however, was accompanied by an elevation of cis-prenyltransferase activity and higher de novo production of dolichols. Despite these compensatory changes, protein glycosylation, cell wall integrity, filamentous growth, and biofilm formation were impaired in the mutant. These results suggest that the defects are not due to the lack of DolP for the protein N-glycosylation but rather that the activity of oligosacharyltransferase could be inhibited by the excess DolPP accumulating in the mutant.

Dolichol

Dephosphorylation

Tunicamycin

N-linked glycosylation

10.3390/ijms20205067

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Candida albicans; exploring glycosylation pathway in the search of targets for antimicrobial agents and yeast to hyphae transition.

Medical Research Archives (2019)

A. Janik Mateusz Juchimiuk Monika Niewiadomska U Lenart Joanna S. Kruszewska

Microbial cell wall is mostly synthesized by the glycosylated proteins with the distinct enzymatic activity. In this review we have concentrated on the description of the certain steps of glycosylation and their effect on the cell wall integrity and yeast to hyphae transition, the process enhancing the pathogenic properties of C.albicans. The glycoproteins play an invaluable role in C. albicans virulence and they modulate adhesive, invasive, morphogenetic and immune stimulating properties of the pathogen as well as its susceptibility to the antifungal agents. Therefore, understanding of C. albicans glycobiology might let us expand the arsenal in the war against fungal enemies. The early stages of N-, O-glycans and GPI-anchor synthesis requires dolichol - the lipid carrier of sugar residues. Diminished supply of dolichol causes series of defects in C. albicans cells, among which aberrant protein glycosylation is the most evident. Furthermore, the relations between the cell wall composition and integrity, resistance to some antifungal and cell wall disturbing agents and dolichol dependent glycosylation are observed. Moreover relevance of these reactions for the morphological differentiation of C.albicans is described.

Dolichol

Glycobiology

10.18103/mra.v7i9.1968

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