Specific primers which were designed based on the sequences of Cp gene and RNA2 by multiplex RT-PCR were used to detect 5 virus,only in RNA extract from plant tissue infected with TRSV and TomRSV,the specific product of 600 bp and 470 bp DNA can be amplificd with their specific primers respectively.The lowest positive signal could be amplified from up to 234 pg/μl TRSV infected leaf total RNA and 264 pg/μl TomRSV infected leaf total RNA.RT-PCR method was showed to be spesific,sensitive,rapid for detction of TRSV and TomRSV.
Abstract Aim Biological invasion has become one of the most important environmental concerns in many countries, with considerable time, money and effort being spent in the prevention and eradication of invasive species. Since mineral ores tend to harbour seeds from the local plants, our aim was to study the non‐native plants collected in Chinese ports to understand the influence of global ore trade on biological invasion. Location China. Methods We surveyed 75 ore heaps across six types of ore in 22 Chinese port cities from 2010 to 2016 and collected 737 voucher specimens of non‐native plants, out of which 709 specimens were traced to the country of origin. Using the software Maxent, we evaluated the risk of invasion from these non‐native plants based on the global ore trade flow, traced their route and predicted the regions in China most vulnerable to invasion by these plants. Results Of the 407 non‐native plant species identified, most were from India, followed by Malaysia, Swaziland, Mexico, and Iran. Taxonomically, there were representations from 49 families, notably Fabaceae, Poaceae, Asteraceae and Solanaceae. The non‐native plant species were represented by varying number of species, from a single specimen to 179. Analysis of the invasion risk indicated that the entire coast of China was at high risk. Furthermore, two major potential introduction pathways were also identified, namely the Yangtze drainage basin and the land from the Gulf of Tonkin to Sichuan Basin. Main conclusion An important pathway for the invasion of non‐native plants is the inadvertent transportation of seeds through global ore trade networks. Based on this study, we suggest greater monitoring and cooperation in the international ore trade for better management, and creating public awareness of the dangers of non‐native species to help minimize the risk of transporting non‐native plants by the global ore trade network.
Abstract Taking Xanthomonas campestris pv. vesicatoria (Doidge) Dye, a pathogen with a wide geographical distribution, as a representative, pyrosequencing is shown for the first time to provide characteristic information of plant pathogenic bacteria strain‐specific sequences. Pyrosequencing‐based plant pathogen detection and typing technology is demonstrated to be rapid, highly specific and more sensitive than conventional technologies. The specificity of such assays has been validated by conventional DNA sequencing and metabolic fingerprinting. It is a starting point for the application and development of pyrosequencing in plant inspection and quarantine which underlie agricultural communication.
Quarantine plant bacteria (QPB) are significant component of invasive alien species that result in substantial economic losses and serious environmental damage. Here, a colorimetric aptasensor has been proposed based on the sandwich structure of an autonomous screening aptamer and the cascaded catalytic strategy of a nanozyme for on-site detecting Xanthomonas hyacinthi, a type of QPB, in natural environments. The self-screened aptamer obtained through SELEX can bind with the binding sites on the surface of viable organism with high affinity and specificity, which guarantees the selectivity of aptasensor. As an important part of the aptasensor, MIL-88-NH2(Fe) not only acts as a multifunctional carrier for both aptamers and glucose oxidase, but also catalyzes enzyme-like reaction because of tremendous specific surface area, plentiful amino and excellent peroxidase-like activity. The present of Xanthomonas hyacinthi can trigger the formation of a sandwich structure and the occurrence of cascade catalytic reaction, enabling the detection with UV spectra and naked eyes. The proposed aptasensor presents a low detection limit of 2 cfu/mL and a wide linear range of 10 -107 cfu/mL. Compared to traditional methods of QPB detection, the reasonable design, high selectivity and convenience significantly improve the detection efficiency and contribute to environmental protection.
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