Twenty four (24) MRSA isolates were obtained from clinical specimens and subjected to genetic study in Babylon Province in a period from July to January2011. Polymerase Chain Reaction was used for detection the genes that responsible for methicillin resistance (mecA, SCCmec type IV) and genes that responsible for toxin production (pvl, lukED). It was found that 24 (100%) isolates have positive result for mecA gene. The MRSA isolates were also SCCmec typed to differentiate between HA-MRSA and CA-MRSA isolates by using a uniplex PCR assay, 23 (95%) were found to be carrying SCCmec type IV, 19 (79%) had positive result for pvl toxin gene, 20 (83%) had lukED toxin gene. ةصلاخلا

SCCmec

Meticillin

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Citations (3)

Possible Protective Effect of Zucchini Juice against Nephrotoxicity Induced by Cisplatin in Mice

Deleted Journal (2020)

Entisar Jawad Al-Mukhtar Asmaa M. Mekkey Zahraa Manji Saleem Huda H. Al-Hasnawy Nisreen Al- Bayati

Nephrotoxicity

10.36295/asro.2020.2365

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Detection of Main Causative Agents among Young Children Suffering from Epiglottitis in Hilla City, Iraq.

PubMed (2023)

Jamal Ahmed Badwy Khalifa Huda H. Al-Hasnawy

Epiglottitis is a rapidly progressive epiglottis infection leading to upper airway edema. This study aimed to detect the main causative agent, viral infection, by immunofluorescence antibody technique and PCR technique and bacterial infection detection by specific gene among young children suffering from epiglottitis. This study included 85 young children aged 10-15 years. The virus was identified on 85 blood samples using the CER test Human simplex virus Card test; the results revealed that 12 (14.1%) specimens were related to virus infection, and the sera of patients showed anti-IgM to HSV-1 antibodies. HSV-1 was detected in blood samples by qPCR technique. Eighty-five saliva samples were collected from young children suffering from epiglottitis. The samples were cultured for 18-24 hours at 37°C. They were then cultivated for 18-24 hours on various selective media at 37°C. The colony morphology, microscopically, and biochemical testing were used to identify Haemophilus influenzae as a first Identification. Out of 85 clinical specimens, 63 (74.1%) were positive culture, while 22 (25.9%) had no growth on culture media; out of 63 specimens, only 22 (34.9%) isolates belonged to Haemophilus influenzae by biochemical tests, while 41 (65.1%) related to other types of microorganisms. VITEK 2 was used to validate bacteria isolates from young children suffering from epiglottitis. The findings indicate that 22 (34.9%) isolates related to Haemophilus influenzae have been confirmed with an excellent ID message confidence level (94 to 99.8% likelihood percentage). This method is characterized by quick bacterial detection. DNA was taken from all suspected isolates previously identified as Haemophilus influenzae using the vitek2 technology, and traditional PCR was used to amplify specific hel gene for Haemophilus influenzae primers utilizing these DNA samples. After that, when compared to an allelic ladder, gel electrophoresis revealed that all 22 (100%) samples of Haemophilus influenzae produced 101 bp DNA fragments. For isolates previously identified as Haemophilus influenzae, molecular identification of the ompP gene was performed. The results showed that 12 (or 54.5 percent) of the 22 isolates tested positive for this virulence gene. When compared to an allelic ladder, the presence of (459 bp) bands indicated positive results. In addition, the bexA gene was molecularly detected in 22 Haemophilus influenzae isolates, showing that only 8 (36.3 percent) of the isolates had this gene. When compared to an allelic ladder, the presence of a (343 bp) band indicated positive results for bexA gene pathogenicity; in conclusion, HSV (1) and Hib were considered almost causative agents of epiglottitis in young children.

Epiglottitis

Epiglottis

10.22092/ari.2022.358161.2165

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Antibacterial and Cytotoxic Effects of Silver Nanoparticles on Staphylococcus aureus and Normal Vero Cells

Deleted Journal (2019)

Rasha Hadi Saleh Entisar J. Al-Mukhtar Zaytoon A. Al-Khafaji Mohammed H. Al Hasnawy Huda H. Al-Hasnawy

Vero cell

Silver nanoparticle

10.36295/asro.2019.221025

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Etiological Agents of Pruritus Vulvae in Prepupertal Girls, In Hilla City

Medical Journal of Babylon (2011)

Kareema A. Al-Khafajii Ban Alwash Huda H. Al-Hasnawy

Etiology

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The age and residence impact on IgE serum level in patients with allergic asthma

Medical Journal of Babylon (2024)

RaghdahMaytham Hameed Huda H. Al-Hasnawy Haidar Abdul Amir Najim Abood

Abstract Background: Immunoglobulin E (IgE) has a role in mediating allergic reactions and their powerful effector functions, but numerous factors influence its value. Objective: To find any difference in total and specific IgE serum levels at the different age groups and residences. Materials and Methods: Eighty-seven asthmatic children, including 57 males and 30 females with asthma aged between 1 and 16 years old, 32.2% living in rural and 67.8% living in urban, were collected at Kerbala Teaching Hospital for Children. All asthmatic children in this study were subjected to measuring total IgE level using AccuBind IgE ELISA kit, Human Chlamydia pneumoniae IgG using Cpn IgG ELISA kit, and Human C. pneumoniae IgE using Cpn IgE ELISA kit. Results: There was a significant positive linear correlation between age and total IgE level and a significant negative correlation between age and C. pneumoniae IgE in asthmatic children (0.255, P = 0.017, -0.233, P = 0.03, respectively). Further, there was a significant positive linear correlation between total IgE and C. pneumoniae IgE under age controlling (0.225, P = 0.019). In urban residents, the asthmatic children more than eleven years old had a low C. pneumonia IgE serum level (5.845 ± 1.821 ng/L) compared with asthmatic children who lived in rural areas (8.206 ± 2.793 ng/L). Depending on age groups, there was a significant difference ( P = 0.047) in C. pneumonia IgE serum levels in asthmatic children who lived in urban areas. Conclusion: C. pneumonia -specific IgE decreased in early adulthood urban asthmatic children.

10.4103/mjbl.mjbl_598_23

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Citations (1)

Molecular Characterization of bla OXA-51 Gene among Acinetobacter baumannii Isolates in Babylon Province

Mağallaẗ kulliyyaẗ ğāmiʿaẗ al-Ḥillaẗ ğāmiʿaẗ li-l-ʿulūm al-ṭibiyyaẗ. (2024)

Huda H. Al-Hasnawy Alya Amer Rahi Baraa Hamid Hadi

Acinetobacter baumannii

10.62445/2958-4515.1014

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Molecular Detection of M. Pneumoniae in Pneumonia Patients in Al-Hilla City, Iraq

Indian Journal of Public Health Research & Development (2019)

Ahmed Ali Huda H. Al-Hasnawy Noor Ul Huda Nabeel

10.5958/0976-5506.2019.02245.9

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