Heba M. Karam

Egyptian Atomic Energy Authority

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Dina M. Lotfy

Egyptian Atomic Energy Authority

Ayman A. Ibrahim

Egyptian Atomic Energy Authority

Aiten M. Soliman

Egyptian Atomic Energy Authority

Mostafa M. Ghorab

Egyptian Atomic Energy Authority

Mai H. Mekkawy

Egyptian Atomic Energy Authority

Esmat A. Shaaban

Egyptian Atomic Energy Authority

Rasha R. Radwan

Egyptian Atomic Energy Authority

B. Housset

Hôpital Intercommunal de Créteil

Maureen Higgins

University of Dundee

Sanaa A. Kenawy

Cairo University

Cooperative Institutions

Egyptian Atomic Energy Authority

Cairo University

National Research Centre

Sorbonne Université

Hôpital Saint-Antoine

Université Paris Cité

Alexandria University

National Water Research Center

Centre Hospitalier Universitaire Henri-Mondor

Inserm

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

MitoQ and its hyaluronic acid–based nanopreparation mitigating gamma radiation–induced intestinal injury in mice: alleviation of oxidative stress and apoptosis

Naunyn-Schmiedeberg s Archives of Pharmacology (2024)

Mohamed Dawoud K. M. Attallah Ismail Ibrahim Heba M. Karam Ayman A. Ibrahim

Malondialdehyde

Mitochondrial ROS

10.1007/s00210-024-02948-5

Cite

Citations (2)

Nrf2 and NF‐қB interplay in tamoxifen‐induced hepatic toxicity: A promising therapeutic approach of sildenafil and low‐dose γ radiation

Environmental Toxicology (2023)

Heba M. Karam Shereen Mohamed Galal Dina M. Lotfy

Tamoxifen-induced hepatotoxicity is an inevitable side effect during breast cancer treatment. Low-dose gamma irradiation (IRR) shows many beneficial effects by stimulating various biological processes. This study evaluates the possible effect of sildenafil and low-dose gamma radiation on liver damages as new treatment strategies. Group I (control), group II: (tamoxifen), group III: (tamoxifen + Sildenafil), group IV: (tamoxifen+ irradiation) and group V: (tamoxifen +Sildenafil + irradiation). Rats were sacrificed after 5 h from tamoxifen injection. Results showed that tamoxifen caused elevation in serum AST, ALT and ALP as well hepatic ROS, iNOS, MDA, Keap-1 and NF-Kb, in addition to diminution in hepatic Nrf2 and HO-1. Exposure to low-dose gamma radiation and sildenafil amended the alterations in the measured parameters in serum and tissue. Moreover, all results were confirmed by histopathological examination. In conclusion, sildenafil and low-dose gamma radiation can mitigate the toxicity induced by tamoxifen in liver tissues. Hence, this treatment could be further evaluated as a new approach for alleviating various liver disorders.

10.1002/tox.23742

Cite

Citations (2)

Antioxidant activity of novel quinazolinones bearing sulfonamide: Potential radiomodulatory effects on liver tissues via NF-κB/ PON1 pathway

European Journal of Medicinal Chemistry (2020)

Aiten M. Soliman Heba M. Karam Mai H. Mekkawy Mostafa M. Ghorab

Acetamide

Moiety

Docking (animal)

10.1016/j.ejmech.2020.112333

Cite

Citations (46)

Molecular modeling and implications of Biochanin A on ghrelin and IGF-1/myostatin signaling in radiation triggered cachexia

Future Journal of Pharmaceutical Sciences (2024)

Aya Shaheen Heba A. Gheita Heba M. Karam Nashwa H. Zaher Walaa A. El‐Sabbagh

Abstract Background Cachexia, a loss of appetite and body weight as a result of systemic inflammation, considered one of the unavoidable side effects of radiation therapy. Controlling ghrelin (Ghr) levels could assist in alleviating this condition by improving appetite, promoting energy storage, and reducing cytokines’ generation. This study aimed to explore the effect of Biochanin A (BCA), a natural bioactive isoflavone, in alleviating radiation-cachexic syndrome. Results Molecular docking study of BCA demonstrated strong fitting with more binding interactions than megestrol acetate (MA), a commonly prescribed medication for cachexia, into Ghr active binding site. Accordingly, irradiated rats were treated with BCA or MA, with body weight monitoring. Force swimming test (FST) was carried out followed by gastrocnemius muscle weighting and histological examination. Biochemical assay of Ghr, TNF-α, insulin growth factor-1 (IGF-1), myostatin (Mst), lactate dehydrogenase (LDH), and brain serotonin (5-HT) level, were carried in order to estimate the possible action pathway of BCA. Results showed that BCA improved weight gain and histological muscle bundle arrangement. Although, BCA and MA significantly reduced serum TNF-α by 25.6% and 24.2%, respectively, only BCA maintained normal IGF-1and Mst levels, whose balance is necessary to avoid skeletal muscle loss, the main mark of cachexia. Moreover, BCA showed tissue injury mitigation with normal energy expenditure by significantly suppressing LDH (20.5%) and maintaining normal 5-HT level. Conclusion By preserving the appropriate IGF-1 and MST metabolic balance and keeping muscle homeostasis, BCA, with its high Ghr binding interaction and anti-inflammatory properties, could have an impact on radiation cachectic syndrome.

Myostatin

Biochanin A

10.1186/s43094-024-00726-x

Cite

Citations (0)

Novel iodinated quinazolinones bearing sulfonamide as new scaffold targeting radiation induced oxidative stress

Bioorganic & Medicinal Chemistry Letters (2021)

Aiten M. Soliman Mai H. Mekkawy Heba M. Karam Maureen Higgins Albena T. Dinkova‐Kostova

Malondialdehyde

10.1016/j.bmcl.2021.128002

Cite

Citations (8)

A new approach of nano-metformin as a protector against radiation-induced cardiac fibrosis and inflammation via CXCL1/TGF-Β pathway

Naunyn-Schmiedeberg s Archives of Pharmacology (2024)

Heba M. Karam Dina M. Lotfy Ayman A. Ibrahim Farag M. Mosallam Sahar S. Abdelrahman

Abstract The present work investigates the potential role of metformin nanoparticles (MTF-NPs) as a radio-protector against cardiac fibrosis and inflammation induced by gamma radiation via CXCL1/TGF-β pathway. Lethal dose fifty of nano-metformin was determined in mice, then 21 rats (male albino) were equally divided into three groups: normal control (G1), irradiated control (G2), and MTF-NPs + IRR (G3). The possible protective effect of MTF-NPs is illustrated via decreasing cardiac contents of troponin, C-X-C motif Ligand 1 (CXCL1), tumor growth factor β (TGF-β), protein kinase B (AKT), and nuclear factor-κB (NF-κB). Also, the positive effect of MTF-NPs on insulin-like growth factor (IGF) and platelet-derived growth factor (PDGF) in heart tissues using immunohistochemical technique is illustrated in the present study. Histopathological examination emphasizes the biochemical findings. The current investigation suggests that MTF-NPs might be considered as a potent novel treatment for the management of cardiac fibrosis and inflammation in patients who receive radiotherapy or workers who may be exposed to gamma radiation. Graphical Abstract

CXCL1

Cardiac Fibrosis

Myocardial fibrosis

10.1007/s00210-024-03052-4

Cite

Citations (1)

Radiomodulatory effect of a non-electrophilic NQO1 inducer identified in a screen of new 6, 8-diiodoquinazolin-4(3H)-ones carrying a sulfonamide moiety

European Journal of Medicinal Chemistry (2020)

Aiten M. Soliman Heba M. Karam Mai H. Mekkawy Maureen Higgins Albena T. Dinkova‐Kostova

Fifteen new quinazolinone derivatives bearing benzenesulfonamide moiety with variable acetamide tail were synthesized. The structures assigned to the products were concordant with the microanalytical and spectral data. Compounds 4–18 were screened for their ability to induce the antioxidant enzyme NAD(P)H: quinone oxidoreductase 1 (NQO1) in cells, a classical target for transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). The 2-((6,8-diiodo-4-oxo-3-(4-sulfamoylphenyl)-3,4-dihydroquinazolin-2-yl)thio)-N-(3,4,5-trimethoxyphenyl) acetamide 15 showed the most potent NQO1 inducer activity in vitro. Compound 15 had low toxicity in mice (LD50 = 500 mg/kg). It also reduced the damaging effects of gamma radiation, as assessed by the levels of Nrf2, NQO1, reactive oxygen species (ROS) and malondialdehyde (MDA) in liver tissues. In addition, compound 15 showed amelioration in the complete blood count of irradiated mice and enhanced survival over a period of 30 days following irradiation. Molecular docking of 15 inside the Nrf2-binding site of Kelch-like ECH associated protein 1 (Keap1), the main negative regulator of Nrf2, showed the same binding interactions as that of the co-crystallized ligand considering the binding possibilities and energy scores. These findings suggest that compound 15 could be considered as a promising antioxidant and radiomodulatory agent.

Acetamide

Moiety

Malondialdehyde

Docking (animal)

10.1016/j.ejmech.2020.112467

Cite

Citations (15)

Improvement of Naja haje snake antivenom production using gamma radiation and a biotechnological technique

Toxin Reviews (2019)

Heba M. Karam Esmat A. Shaaban Aly Fahmy Hala F. Zaki Sanaa A. Kenawy

This study aimed to improve antivenom production using gamma radiation and calcium phosphate nanoparticles (CPN) as adjuvant. Toxicological and immunological properties of the Naja haje venom before and after exposure to radiation were estimated. The immune response of immunized rabbits was evaluated. Toxicity of irradiated venom was reduced six times as compared to native venom. Moreover, no change in antigenic reactivity between native and irradiated venoms. Serum titer produced with irradiated venom loaded on CPN showed highest titer and neutralization capacity as compared to other sera. Thus, irradiation and nanoparticles adjuvant could be a new approach for snake antivenom production.

Antivenom

10.1080/15569543.2019.1700381

Cite

Citations (5)

Review On Some Heavy Metals Toxicity On Freshwater Fishes

Journal of Applied Veterinary Sciences/Journal of Applied Veterinary Sciences (2020)

Shahbaa K. AL Taee Heba M. Karam Hana Kh. Ismail

Heavy metals contamination is a serious problem in the aquatic environment. Some of them are very important, having biological roles for aquatic organisms, and called essential heavy metals. In contrast, other heavy metals are considered harmful even at low concentrations. The toxic levels of heavy metals may be of agricultural, industrial and mining activities. This will cause water pollution and changes in the physicochemical characteristics of the aquatic environment. This pollution has deleterious toxic effects on fish and raises concerns over its potential impact on human health. The most common heavy metals are arsenic, chromium, lead and mercury, which affect human health and are considered systemic toxicants. These metals induce organ damage even at low levels of exposure and according to the US Environmental protection agency and international agency for research on cancer, they classified as carcinogens. For all the above reasons, this review was written to contribute to heavy metals' role in the environment, toxic mechanism and toxic effects on fish.

Fresh water fish

10.21608/javs.2020.100157

Cite

Citations (29)

The potential protective effect of Ferulic acid against gamma irradiation induced ovarian failure in rats

Egyptian Journal of Radiation Sciences and Applications (2019)

Heba M. Karam salma Abdel Fattah Marwa Mohamed

T hIs sTuDy aims to evaluate the radioprotective effect of Ferulic acid (FA) against ovarian damage in premature female rats.Four groups of female rats were used (ten rats in each group).Group (1): Control, Group (2): Irradiation (IRR) (rats were exposed to 4Gy of whole body gamma irradiation as a single dose), Group (3): FA (rats received 50mg/kg b.wt of Ferulic acid orally for two weeks) and Group (4): FA+IRR (rats received Ferulic acid as group 3 then exposed to 4Gy as group2).The rats were sacrificed after 24h of irradiation.The level of FSH, LH, and E 2 were estimated in the serum.The level of H 2 O 2 and GPx activity and the gene expression of cytochrome C, P53 and caspase3 were evaluated in ovarian tissue.Histopathological examination of the ovary and uterus tissues was performed.The results revealed that gamma irradiation caused an elevation in FSH, LH, H 2 O 2 , cytochrome C, P53 and caspase3 and a decline in E 2 and GPx.The administration of FA prior gamma irradiation led to an improvement in female hormones, H 2 O 2 , GPx and apoptotic markers and matched the histopathological examination results.It could be concluded that Ferulic acid may exert a radioprotective effect against irradiation induced apoptosis in the ovary of rats and alteration in the level of serum female hormones.

10.21608/ejrsa.2019.6608.1058

Cite

Citations (4)