Michael W. Russell

University at Buffalo, State University of New York

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jiří Městecký

University of Alabama at Birmingham

George Hajishengallis

University of Pennsylvania

Suzanne M. Michalek

University of Alabama at Birmingham

Zina Moldoveanu

University of Alabama at Birmingham

Thomas A. Brown

Alnylam Pharmaceuticals (United States)

Mogens Kilian

Aarhus University

Thomas Lehner

New York Genome Center

Hong‐Yin Wu

University of Alabama at Birmingham

John W. Connell

University at Buffalo, State University of New York

Edward W. Hook

University of Alabama at Birmingham

Cooperative Institutions

University of Alabama at Birmingham

134

National Institutes of Health

Harvard University

Karolinska Institutet

University at Buffalo, State University of New York

University of Pennsylvania

University of Alabama

University of Washington

Czech Academy of Sciences, Institute of Microbiology

Cornell University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

CCDC 2265734: Experimental Crystal Structure Determination

The Cambridge Structural Database (2023)

Lee J. Silverberg Tapas K. Mal Carlos Pacheco Hemant P. Yennawar Anthony F. Lagalante

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/ccdc.csd.cc2g1p6f

Cite

Citations (0)

Affinity purification and characterization of protease-susceptible antigen I of Streptococcus mutans

Infection and Immunity (1980)

Michael W. Russell Edward D. Zanders Lesley A. Bergmeier Thomas Lehner

An antigenic component (antigen I) of the cell surface of Streptococcus mutans has been purified from culture supernatants and shown to be immunologically identical to the protease-susceptible moiety of antigen I/II. Ion-exchange and gel filtration chromatography failed to yield a physicochemically homogeneous product. Immunoasbsorbent chromatography on single and tandem columns containing immobilized antibodies to antigens I/II and II yielded identical products which were homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and which when injected into rabbits induced monospecific antisera to antigen I. This antigen consisted of approximately 70% protein. Its molecular weight was estimated as 150,000, and the isoelectric point was estimated to be 5.1. Immunofluorescence microscopy using monospecific antiserum to antigen I showed that a similar antigen was present on cells of S. mutans serotypes a, c, d, e, f, and g, but not b.

Sodium dodecyl sulfate

10.1128/iai.29.3.999-1006.1980

Cite

Citations (38)

Protective Immunity against Streptococcus mutans Infection in Mice after Intranasal Immunization with the Glucan-Binding Region of S. mutans Glucosyltransferase

Infection and Immunity (1999)

Christina Jespersgaard George Hajishengallis Yan Huang Michael W. Russell Daniel J. Smith

ABSTRACT Here we present the construction and characterization of a chimeric vaccine protein combining the glucan-binding domain (GLU) of the gtfB -encoded water-insoluble glucan-synthesizing glucosyltransferase enzyme (GTF-I) from Streptococcus mutans and thioredoxin from Escherichia coli , which increases the solubility of coexpressed recombinant proteins and stimulates proliferation of murine T cells. The protective potential of intranasal (i.n.) immunization with this chimeric immunogen was compared to that of the GLU polypeptide alone in a mouse infection model. Both immunogens were able to induce statistically significant mucosal (salivary and vaginal) and serum responses ( P < 0.01) which were sustained to the end of the study (experimental day 100). Following infection with S. mutans , sham-immunized mice maintained high levels of this cariogenic organism (∼60% of the total oral streptococci) for at least 5 weeks. In contrast, animals immunized with the thioredoxin-GLU chimeric protein (Thio-GLU) showed significant reduction (>85%) in S. mutans colonization after 3 weeks ( P < 0.05). The animals immunized with GLU alone required 5 weeks to demonstrate significant reduction (>50%) of S. mutans infection ( P < 0.05). Evaluation of dental caries activity at the end of the study showed that mice immunized with either Thio-GLU or GLU had significantly fewer carious lesions in the buccal enamel or dentinal surfaces than the sham-immunized animals ( P < 0.01). The protective effects against S. mutans colonization and caries activity following i.n. immunization with GLU or Thio-GLU are attributed to the induced salivary immunoglobulin A (IgA) anti-GLU responses. Although in general Thio-GLU was not significantly better than GLU alone in stimulating salivary IgA responses and in protection against dental caries, the finding that the GLU polypeptide alone, in the absence of any immunoenhancing agents, is protective against disease offers a promising and safe strategy for the development of a vaccine against caries.

Glucosyltransferases

Immunogen

10.1128/iai.67.12.6543-6549.1999

Cite

Citations (54)

Assessment of Cytochrome P450 3A4 Activity During the Menstrual Cycle Using Alfentanil as a Noninvasive Probe

Survey of Anesthesiology (1998)

Evan D. Kharasch Michael W. Russell Kyle J. Garton Gretchen M. Lentz Andrew Bowdle

KHARASCH, EVAN D.; RUSSELL, MICHAEL; GARTON, KYLE; LENTZ, GRETCHEN; BOWDLE, T. ANDREW; COX, KATHY Author Information

Alfentanil

10.1097/00132586-199810000-00046

Cite

Citations (7)

Non‐invasive stool‐based detection of newborn infant gastrointestinal development using gene expression profiles derived from exfoliated epithelial cells

The FASEB Journal (2010)

Sharon M. Donovan Laure A Davidson Chen Zhao Ivan Ivanov Jennifer S. Goldsby

Understanding of the molecular regulation of intestinal development by human milk has been limited by the lack of non‐invasive approaches. Herein, a novel molecular methodology utilizing stool samples containing intact sloughed epithelial cells was used to identify gene sets (combinations) that were differentially regulated in response to infant feeding. Fecal mRNA was isolated from 3‐month‐old exclusively breast (BF; n=12) and formula (FF; n=10) fed infants. Linear discriminant analysis was used to identify the best single genes and two‐ to three‐gene combinations for distinguishing the feeding groups. EPAS‐1, a transcription factor involved in inducing oxygen‐regulated genes, was identified as a putative “master” regulatory gene using coefficient of determination analysis. Bioinformatic analysis identified differentially regulated processes associated with apoptosis, chemotaxis and development (Wnt and NOTCH pathways). Thus, epithelial cell mRNA isolated from stool has value in terms of characterizing the mechanisms underlying developmentally‐regulated transcriptional activation/repression of genes known to modulate gastrointestinal development. (Funded by Mead Johnson and NIH CA59034, CA129444 , DK71707 and P30ES09106)

10.1096/fasebj.24.1_supplement.206.6

Cite

Citations (0)

Biological Activities of IgA

Elsevier eBooks (2015)

Michael W. Russell Mogens Kilian Nicholas J. Mantis Blaise Corthésy

10.1016/b978-0-12-415847-4.00021-5

Cite

Citations (113)

HYPERCOAGULABILITY IN A CLINICALLY RELEVANT MODEL OF SEPSIS

Critical Care Medicine (1999)

Cynthia M. Otto Marjory B. Brooks Theresa M. Rieser Michael W. Russell

Society Of Critical Care Medicine 29Th Educational And Scientific Symposium; Orlando, Florida, Usa; February 11-15, 2000: Poster Presentations

10.1097/00003246-199912001-00266

Cite

Citations (0)

Mucosal Immunity in Sexually Transmitted Infections

Jiří Městecký Michael W. Russell

Although the immune compartment of the genital tract is considered a component of the common mucosal system, it displays distinct features characteristic of its physiological and immunological needs not shared by other mucosal sites. These include marked differences in the phenotypes of epithelial cells, lymphocytes, macrophages and dendritic cells, as well as isotype distribution and origin of antibodies in genital tract secretions. The absence of organized inductive lymphoepithelial mucosal tissues analogous to those present in the intestinal and respiratory tracts compromises the induction of vigorous immune responses not only to agents of sexually transmitted diseases, but also to allogeneic sperm and a semi-allogeneic fetus which are essential for species survival. Furthermore, based on studies of the induction of humoral and cellular immune responses and their pronounced hormonal regulation, it has become obvious that future immunization strategies must consider the uniqueness of the genital tract immune system.

Genital tract

Mucosal immunology

Mucosal Immunity

Respiratory tract

Sex organ

10.1002/9781119380924.ch1

Cite

Citations (1)

Tolerance and Protection against Infection in the Genital Tract

Immunological Investigations (2010)

Michael W. Russell Jiří Městecký

AbstractThe genital tract is a unique immunological environment that must support the reproductive function and resist infection. Particularly in the female tract, immunoregulatory and immunosuppressive activities that permit the growth of the fetus create an environment that can readily be exploited by microbes that have become well-adapted to this location. Cellular and molecular mediators of immune responses differ from those found at other mucosal surfaces. Mechanisms of immune response induction and delivery, as well as immune effector functions at the genital mucosae need to be considered in the development of vaccines against infections of the genital tract.Keywords: Genital mucosaImmunityToleranceInfectionVaccination ACKNOWLEDGMENTSStudies in the authors' laboratories are supported by NIH grants DE06746 and AI074791 (to MWR), AI083027 and AI028147 (to JM), a grant from the John R. Oishei Foundation (to MWR), and grant VZMSM 0021620812 from the Czech Republic (to JM).Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Genital tract

Female circumcision

Sex organ

Declaration

10.3109/08820131003674834

Cite

Citations (29)

Mucosal immunogenicity of a recombinant Salmonella typhimurium-cloned heterologous antigen in the absence or presence of coexpressed cholera toxin A2 and B subunits

Infection and Immunity (1997)

Evlambia Harokopakis George Hajishengallis T. Greenway Michael W. Russell Suzanne M. Michalek

An avirulent Salmonella typhimurium vaccine strain expressing a streptococcal protein adhesin and a similar clone which produces the same streptococcal antigen linked to the cholera toxin (CT) A2 and B subunits (CTA2/B) were compared for the ability to induce antibody responses to the expressed heterologous antigen after oral or intranasal immunization of mice. Expression of cloned immunogens in these systems is temperature regulated, being optimal at 37 degrees C, and the two clones under comparison were shown to produce similar levels of the streptococcal antigen. Both clones were found to stimulate high levels of serum immunoglobulin G (IgG) and mucosal IgA antibodies to the cloned immunogen. A consistent trend was observed toward higher mucosal IgA but lower serum IgG responses in the case of the S. typhimurium vector that coexpressed CTA2/B, a potential mucosal adjuvant, regardless of the route of administration. Also noteworthy was the capacity of these antigen delivery systems to induce anamnestic systemic and secretory responses to the cloned immunogen 15 weeks after the primary immunization, despite preexisting immunity to the Salmonella vectors. These antibody responses were sustained for at least 7 months following the booster immunization, at which time the secretory IgA antibody levels were significantly higher in mice given the Salmonella clone that coexpressed CTA2/B. Although the serum IgG response against the Salmonella vector was characterized by a high IgG2a/IgG1 ratio (indicative of the T helper type 1 [Th1]/Th2 profile), a mixed IgG1 and IgG2a pattern was observed for the carried heterologous antigen, which displayed a dominant IgG1 response when administered as a purified immunogen. Our findings indicate that the recombinant streptococcal antigen and CTA2/B are strong immunogens when expressed by the antigen delivery system used in this study and suggest that CTA2/B may have an additional immunoenhancing activity in the mucosal compartment besides its ability to target antigen uptake into the mucosal inductive sites. CTA2/B may thus be useful as an S. typhimurium-cloned adjuvant for coexpressed protein antigens.

Immunogen

Cholera toxin

Heterologous

Immunoglobulin A

10.1128/iai.65.4.1445-1454.1997

Cite

Citations (53)