Gregorio Hueros

Universidad de Alcalá

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Joaquı́n Royo

Universidad de Alcalá

Elisa Gómez

Universidad de Alcalá

Luís M. Muñiz

Universidad de Alcalá

Esther Ferrer

Universidad de Alcalá

Wyatt Paul

Limagrain (France)

Cristina Barrero‐Sicilia

Rothamsted Research

Francesco Salamini

Fondazione Edmund Mach

Monika Maitz

KWS Saat (Germany)

N. Jouve

Universidad de Alcalá

Serena Varotto

University of Padua

Cooperative Institutions

Universidad de Alcalá

Centre National de la Recherche Scientifique

University of Florida

Biogemma (France)

Max Planck Institute for Plant Breeding Research

Université Claude Bernard Lyon 1

Centro Nacional de Biotecnología

United States Department of Agriculture

Laboratoire de Reproduction et Développement des Plantes

Consejo Superior de Investigaciones Científicas

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MaizeRough Endosperm3Encodes an RNA Splicing Factor Required for Endosperm Cell Differentiation and Has a Nonautonomous Effect on Embryo Development

The Plant Cell (2011)

Romain Fouquet Federico Martin Diego Fajardo Christine M. Gault Elisa Gómez

Abstract Endosperm and embryo development are coordinated via epigenetic regulation and signaling between these tissues. In maize (Zea mays), the endosperm–embryo signals are not known, but endosperm cellularization is a key event for embryos to form shoots and roots. We screened seed mutants for nonautonomous functions in endosperm and embryo development with genetically nonconcordant seeds and identified the recessive mutant rough endosperm3 (rgh3). The wild-type Rgh3 allele is required in the endosperm for embryos to develop and has an autonomous role in embryo and seedling development. Endosperm cell differentiation is defective in rgh3. Results from endosperm cell culture indicate that rgh3 mutants remain in a proliferative state through mid-seed development. Rgh3 encodes the maize U2AF35 Related Protein (URP), an RNA splicing factor involved in both U2 and U12 splicing. The Rgh3 allele produces at least 19 alternative splice variants with only one isoform encoding a full-length ortholog to URP. The full-length RGH3α isoform localizes to the nucleolus and displays a speckled pattern within the nucleoplasm, and RGH3α colocalizes with U2AF65. A survey of alternatively spliced transcripts found that, in the rgh3 mutant, a fraction of noncanonical splicing events are altered. Our findings suggest that differentiation of maize endosperm cell types is necessary for embryos to develop. The molecular cloning of Rgh3 suggests that alternative RNA splicing is needed for cell differentiation, development, and plant viability.

Splicing factor

10.1105/tpc.111.092163

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Citations (57)

Cell wall invertase activity regulates the expression of the transfer cell-specific transcription factor ZmMRP-1

Planta (2017)

Diego Bergareche Joaquı́n Royo Luís M. Muñiz Gregorio Hueros

Secondary cell wall

10.1007/s00425-017-2800-y

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Citations (15)

A PCR-based forward genetics screening, using expression domain-specific markers, identifies mutants in endosperm transfer cell development

Frontiers in Plant Science (2014)

Luis M. MuÃ±iz Elisa GÃ mez Virginie Guyon Maribel LÃ pez Bouchaib Khbaya

Mutant collections are an invaluable source of material on which forward genetic approaches allow the identification of genes affecting a wide variety of biological processes. However, some particular developmental stages and morphological structures may resist analysis due to their physical inaccessibility or to deleterious effects associated to their modification. Furthermore, lethal mutations acting early in development may escape detection. We have approached the characterization of 101 maize seed mutants, selected from a collection of 27,500 visually screened Mu-insertion lines, using a molecular marker approach based on a set of genes previously ascribed to different tissue compartments within the early developing kernel. A streamlined combination of qRT-PCR assays has allowed us to preliminary pinpoint the affected compartment, establish developmental comparisons to WT siblings and select mutant lines with alterations in the different compartments. Furthermore, clusters of markers co-affected by the underlying mutation were identified. We have analyzed more extensively a set of lines presenting significant variation in transfer cell-associated expression markers, and have performed morphological observations, and immunolocalization experiments to confirm the results, validating this approach as an efficient mutant description tool.

Forward genetics

10.3389/fpls.2014.00158

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Citations (4)

A structural and evolutionary analysis of a dispersed repetitive sequence

Plant Molecular Biology (1993)

Gregorio Hueros Yolanda Loarce Esther Ferrer

Subcloning

Repeated sequence

Sequence (biology)

Genomic Organization

Hordeum

10.1007/bf00047404

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Citations (19)

Molecular characterization and chromosome location of repeated DNA sequences in Hordeum species and in the amphiploid tritordeum (×Tritordeum Ascherson et Graebner)

Genome (1995)

Esther Ferrer Yolanda Loarce Gregorio Hueros

Genomic DNA from 19 species and subspecies representing the four basic genomes (H, I, X, and Y) of Hordeum was restricted with HaeIII and hybridized with two repeated DNA sequences of Hordeum chilense. The potential use of repeated sequences in ascertaining genomic affinities within the genus Hordeum was studied by comparing restriction fragment patterns. The study demonstrated the following: (i) species that shared a basic genome showed more similar hybridization fragment patterns than species with different genomes, whether with pHchl or pHch3; (ii) hybridization with pHchl revealed the presence of certain fragments limited to the species with a H genome; and (iii) the alloploid nature of species like H. jubatum was confirmed. The chromosomal distribution of the two repeated sequences was studied in species representing each basic genome and in the amphiploid tritordeum using fluorescent in situ hybridization. No interspecific differences were found between the diploid species. In situ experiments indicated the alloploid nature of H. depressum. Both sequences allow H. chilense chromatin to be distinguished from wheat chromosomes in tritordeum.Key words: repeated DNA sequences; in situ hybridization, Hordeum, tritordeum.

Hordeum

Repeated sequence

Genomic Organization

genomic DNA

Subspecies

10.1139/g95-112

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Citations (8)

Gliadin gene location and C-banding identification of Aegilops longissima chromosomes added to wheat

Genome (1991)

Gregorio Hueros Juan M. González J. C. Sanz Esther Ferrer

Gliadin protein components from Aegilops longissima were separated by two-dimensional electrophoresis. No equivalents for α-gliadin were noted. Addition and substitution lines of Ae. longissima in Triticum aestivum 'Chinese Spring' allowed the identification of homoeologous Gli-1 and Gli-2 loci in Ae. longissima chromosomes 1S 1 and 6S 1 . The chromosomal constitution of the alien addition lines was ascertained by C-banding. In addition, C-banding analysis revealed that the Ae. longissima addition set was incomplete as only six distinct addition lines were identified. No evidence for structural modifications between the alien chromosomes in the lines and their Ae. longissima counterparts was found.Key words: gliadins, C-banding, gene location, Aegilops longissima, wheat.

Aegilops

Gliadin

Aegilops tauschii

Common wheat

10.1139/g91-037

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Citations (10)

Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat

Theoretical and Applied Genetics (1988)

J. C. Sanz Gregorio Hueros Juan M. González N. Jouve

Gliadin

Triticale

Glutenin

10.1007/bf00273684

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Citations (2)

Subcellular localisation of BETL-1, -2 and -4 in Zea mays L. endosperm

Sexual Plant Reproduction (2002)

Giampiero Cai Claudia Faleri Cecilia Del Casino Gregorio Hueros R. Thompson

10.1007/s00497-002-0141-9

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Citations (19)

Atypical response regulators expressed in the maize endosperm transfer cells link canonical two component systems and seed biology

BMC Plant Biology (2010)

Luís M. Muñiz Joaquı́n Royo Elisa Gómez Gaelle Baudot Wyatt Paul

Abstract Background Two component systems (TCS) are phosphotransfer-based signal transduction pathways first discovered in bacteria, where they perform most of the sensing tasks. They present a highly modular structure, comprising a receptor with histidine kinase activity and a response regulator which regulates gene expression or interacts with other cell components. A more complex framework is usually found in plants and fungi, in which a third component transfers the phosphate group from the receptor to the response regulator. They play a central role in cytokinin mediated functions in plants, affecting processes such as meristem growth, phyllotaxy, seed development, leaf senescence or tissue differentiation. We have previously reported the expression and cellular localization of a type A response regulator, ZmTCRR-1 , in the transfer cells of the maize seed, a tissue critical for seed filling and development, and described its regulation by a tissue specific transcription factor. In this work we investigate the expression and localization of other components of the TCS signalling routes in the maize seed and initiate the characterization of their interactions. Results The discovery of a new type A response regulator, ZmTCRR-2 , specifically expressed in the transfer cells and controlled by a tissue specific transcription factor suggests a previously unknown role for TCS in the biology of transfer cells. We have characterized other canonical TCS molecules, including 6 histidine kinases and 3 phosphotransfer proteins, potentially involved in the atypical transduction pathway defined by ZmTCRR-1 and 2 . We have identified potential upstream interactors for both proteins and shown that they both move into the developing endosperm. Furthermore, ZmTCRR-1 expression in an heterologous system ( Arabidopsis thaliana ) is directed to xylem parenchyma cells, probably involved in transport processes, one of the major roles attributed to the transfer cell layer. Conclusions Our data prove the expression of the effector elements of a TCS route operating in the transfer cells under developmental control. Its possible role in integrating external signals with seed developmental processes is discussed.

Link (geometry)

Component (thermodynamics)

10.1186/1471-2229-10-84

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Citations (36)

A molecular linkage map of rye

Theoretical and Applied Genetics (1996)

Yolanda Loarce Gregorio Hueros Esther Ferrer

Genetic linkage map

Linkage (software)

Genetic linkage

Plant biochemistry

10.1007/bf00230133

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Citations (76)