In Vietnam, the population of Duttaphrynus melanostictus in the wild has been declined due to overharvesting for snake farms and consumption and environmental pollution. While Black-lipped Toad in cativity rarely ovulates spontaneuosly. Therefore, it’s necessary to study the assisted reproductive techniques to help preserve them. In this study, we used LHRHa and HCG to stimulate induction of spermiation and of spawning of toad. Male were injected with 2 µg/g LHRHa or 3 IU HCG/g to stimulate sperm release. Female were divided in two groups: the first group was administred a single dose of 500 IU HCG (considered as unpriming group) and the second group was administred first two sequential anovulatory dose of 100 IU HCG, repeat after 72 hours. Forty-eight hours after the second injection, the ovulatory dose of 500 IU HCG was injected (priming group). The results showed that injection of LHRHa and HCG stimulates sperm release graetest in time from 5-7 hours after injection. Five hours after injection of LHRHa or HCG, toad sperm concentration was 2.6 and 1.96 million/ml, sperm motility was 82.5 and 85%. Unprimed females failed to spawn any eggs. Whereas, 60% of primed females spawned in the period from 14-16 hours after the last injection of HCG.
This research was performed on 60 semen samples collected from four Phanrang breed rams at the Sontay goat and rabbit research Centre (Hatay province). After the collection, only the samples with good progressive motion (> 70%) were accepted and diluted into diluents. The finally dilute rate was 1:6 (semen:diluent). The temperature of diluents when they were diluted into the semens was 34oC and the stored temperature was 5oC. Research was performed on four diluents: TR diluent (Tris-citric acid-fructose-egg yolk-penicillin and streptomycin), TR diluent with 1% Glycerol, TR diluent with 5% glycerol and TR diluent with 6% DMSO. The progressive motion, the survival rate and the abnormal rate of spermatozoa were evalued during the storage time. The results showed that the TR diluent had maitained a good viability of spermatozoa. Semens stored in the diluents, which had been added either with 1% glycerol or 6% DMSO, had good viability uper 96 hours. The addition of 5% glycerol into the TR diluent reduced its ability to maitain the viability of permatozoa after 72 hours of storage.
Local yellow cattle breed still made up a high percentage of total cattle population in Vietnam. These breed should be inseminated with exotic breed to increase their genetic merit. As the cattle bred in smallholder farmers display estrus sporadic and unconcentrated, application of artificial insemination (AI) tends to limit to areas in close proximity to urban city. Therefore, solutions should be found to enhance the possibilities for using artificial insemination. This paper reports the application of estrus synchronization for artificial insemination of beef cattle. A total of 524 local yellow and Sind crossbreed cattle were assigned randomly into one of two experimental groups. Group 1: cows received 2 ml of PGF2α, followed in 11 d with 2 ml PGF2α and 500 UI PMSG on the same day. Group 2: cows were administered 100 µg GnRH, followed in 7 d with 2 ml of PGF2α. After the last injection in each group, cows were observed for estrus twice daily and those displaying estrus were artificial inseminated. At the end of experiment, rates of detected estrus, conception and parturition in the group 1 and 2 are 84.90 and 82.08%, 82.88 and 80.28%, 93.52 and 92.98% respectively. The results showed that synchronization of estrus has the potential to enhance the possibilities for using AI and help to improve productivity and quality of beef cattle.
The aims of this study was to determine some biocharacteristics of dog semen and to assess the effects of time, environment and others on quality of semen samples. Semen was obtained from professional dog breeds of Berger, Labrador and Cocker at Professional dog research center - Ministry of Public Security. The semen quality of each ejaculate was assessed after collection and the following parameters were determined: volume, pH, sperm concentration, progressive motility, percentage of live spermatozoa and percentage of abnormal spermatozoa. The study was also performed to assess the effects of environmental factors (especially temperature), the period of times between two time of semen collection and the method of semen collection on quality of semen. The results showed that, cold and especially hot weather had negative influence on semen quality. Semen which was collected in summer (from june to august) showed a lower quality (progressive motility, sperm concentration, proportion of live spermatizoa and proportion of abnomal spermatozoa were: 69.00 ± 2.24, 195.47 ± 15.05, 82.26 ± 8.40 and 24.15 ± 3.58, respectively. August 2005) then those in autumn (progressive motility, sperm concentration, proportion of live spermatizoa and proportion of abnomal spermatozoa were: 76.44 ± 9.47, 265.57 ± 53.50, 87.52 ± 8.46 and 16.73 ± 3.9, respectively. November 2005) with cool weather. The period between two times of semen collection and the phase of collection also had a significant effect on semen quality. This study indicated that to obtain semen with high quality, the period between two times of collection might be three days in minimum. We also found that semen which was collected at second phase of ejaculating (progressive motility, sperm concentration, proportion of live spermatizoa and proportion of abnomal spermatozoa were: 74.286 ± 3.450, 318.406 ± 38.617, 84.024 ± 3.770 and 16.686 ± 0.910, respectively) showed a higher quality than was done from all three phases (progressive motility, sperm concentration, proportion of live spermatizoa and proportion of abnomal spermatozoa were: 45.000 ± 7.071, 47.101 ± 18.627, 66.024 ± 4.503 and 16.843 ± 1.751, respectively). These results play an important role on the successful of preservation of dog semen and artificial insemination.
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