E. T. Welch

University of Wisconsin–Madison

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W. A. Neal

Augusta University

J. P. Lewis

United States Department of Veterans Affairs

C.‐S. Wright

Augusta University

Russell R. Moores

Children's Hospital of Richmond at VCU

Coit M. DuBose

Oklahoma Medical Research Foundation

William G. Lewis

Alabama State University

Linda L. Smith

Oregon Health Authority

L. L. Smith

East Carolina University

Dorothy A. Alford

Washington DC VA Medical Center

C.L. Lutcher

Augusta University

Cooperative Institutions

Augusta University

United States Department of Veterans Affairs

University of Tennessee Medical Center

University of Tennessee at Knoxville

University of Wisconsin–Madison

Richard L. Roudebush VA Medical Center

Indiana University – Purdue University Indianapolis

The Ohio State University

University of Georgia

Baylor College of Medicine

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Erythropoietin assay with posthypoxic-polycythemic mice.

PubMed (1970)

J. P. Lewis W. A. Neal Dorothy A. Alford Russell R. Moores E. Gardner

Iron Isotopes

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Citations (11)

Response in mice to erythropoiesis regulatory factors.

PubMed (1973)

J. P. Lewis C.‐S. Wright Russell R. Moores W. A. Neal E. T. Welch

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Inhibition of erythropoiesis by plasma component(s) from sheep, goats, and rabbits.

PubMed (1979)

W. A. Neal J. P. Lewis E. T. Welch C.L. Lutcher Russell R. Moores

Plasma from hypertransfused and normal sheep and experimentally induced anemic and normal goats was fractionated by ultrafiltration. Fractions obtained were assayed for erythropoiesis stimulatory factor (ESF) or erythropoiesis inhibitory factor (EIF) activity (or both) in the posthypoxic polycythemic mouse assay. The most potent sheep plasma-inhibitor fraction was found in the retentate on a membrane with a cutoff at mol wt 50,000. The most potent EIF fraction from anemic goats passed into the ultrafiltrate, but the comparable EIF from normal goats remained in the retentate on a membrane with a cutoff at mol wt 500. The yield of the most potent EIF fraction was higher from anemic goats than was the yield from normal goats. During thin-layer chromatography, EIF extracted from goat plasma fractions had the same mobility as did prostaglandin F2 alpha. Cohn rabbit plasma fraction IV-4 had an inhibitory factor, and a benzene extract of the fraction contained a component that had the same mobility as did prostaglandin F2 alpha. Cohn rabbit plasma fraction V contained a component that potentiated an erythropoietin-generating factor.

Ultrafiltration (renal)

Fraction (chemistry)

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Citations (5)

A protein inhibitor of erythropoiesis.

PubMed (1969)

J. P. Lewis W. A. Neal Russell R. Moores Edward Gardner Dorothy A. Alford

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Citations (26)

Goats as a source of serum erythropoietin.

PubMed (1970)

J. P. Lewis W. A. Neal E. T. Welch Russell R. Moores E. Gardner

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A comparison of four preparations of erythropoiesis regulatory factors

Biochemical Medicine (1975)

J. P. Lewis E. T. Welch W. A. Neal C.‐S. Wright E. Gardner

10.1016/0006-2944(75)90059-9

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Citations (7)

Immobilization and kinetic studies of an erythropoietin-generating factor

Biochemical Medicine (1977)

Coit M. DuBose E. T. Welch J. P. Lewis W. A. Neal C.L. Lutcher

Factor (programming language)

10.1016/0006-2944(77)90037-0

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Citations (5)

エリトロポイエチン(ESF)存在におけるESF産生因子(EGF)の選択的不活性化

J. P. Lewis E. T. Welch W. A. Neal Russell R. Moores W G Lewis

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The Selective Inactivation of an ESF-Generating Factor (EGF) in the Presence of Erythropoietin (ESF)

Experimental Biology and Medicine (1971)

J. P. Lewis E. T. Welch W. A. Neal Russell R. Moores William G. Lewis

An ESF-generating factor (EGF), erythropoietin (ESF) and a mixture of both were incubated with dithiothreitol (DTT, a protective reagent for sulfhydryl groups). Ten μM DTT completely prevented the production of 0.26 ± 0.01 IU of ESF by EGF but did not inactivate ESF. DTT was used to indicate the relative amounts of EGF and ESF activity in a mixture of both.

Dithiothreitol

10.3181/00379727-137-35666

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Citations (2)

The apparent heterogeneity of erythropoietin

Biochemical Medicine (1978)

W. A. Neal E. T. Welch J. P. Lewis C.L. Lutcher C.‐S. Wright

Dithiothreitol

10.1016/0006-2944(78)90069-8

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Citations (9)