Desong Zhu

Shandong Agricultural University

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i-10 index

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Wei Jiang

Shandong University

Shiyun Ai

Shandong Agricultural University

Lei Wang

Qingdao University of Science and Technology

Xiaowen Xu

Shandong University

Xiuling Chu

Liaocheng University

Wei Jiang

Shandong University

Jing Zhu

Xinjiang Academy of Agricultural Sciences

Min Liu

Shandong Normal University

Lingrang Kong

Shandong Agricultural University

Chuan Zhang

Jiangsu University

Cooperative Institutions

Shandong University

Beijing Municipal Ecological and Environmental Monitoring Center

Chongqing Medical University

State Key Laboratory of Microbial Technology

Shandong Agricultural University

Ministry of Agriculture and Rural Affairs

Nankai University

Qingdao University of Science and Technology

Jiangsu University

Xinjiang Academy of Agricultural Sciences

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Moderate Stability of Scissor Double Fluorescent Triple Helix Molecular Switch for Ultrasensitive Biosensing of Crop Transgene

SSRN Electronic Journal (2021)

Xiuling Chu Desong Zhu Min Liu Lingrang Kong Shiyun Ai

Bt transgene, a specific crop DNA sequence, has attracted wide attention because of its introduction into crops for insect control. Meanwhile, it has been gradually drawing concern to crop safety. In this study, a scissor double fluorescent triple helix molecular switch (DFTHMS) based on triple helix DNA with alternating T-A◦T and C-G◦C triads -[-T-A◦T/C-G◦C/T-A◦T/C-G◦C-]n- has been proved to be moderately stable, which have been successfully used for ultrasensitive biosensing of Bt transgene. The influencing factors and changing rules of the stability of triple helix DNA were also systematically studied firstly. The detection results are quite encouraging. Compared with the reported methods, the nanosensor based on this scissor molecular switch possessed several attractive characteristics: (i) The sensitivity was 10,000 times higher than that of traditional methods by efficient dual fluorescence resonance energy transfer (FRET). (ii) It showed generality in the constructions of a series of biological sensing systems for special genes due to the convenient design of this scissor switch by changing the corresponding recognition sequence (human cancer gene, for example, have been successfully detected by this method). (iii) It only needed simple operation process and mild detection conditions since no enzymes were involved, which could greatly save the time of other researchers and the research difficulty of interdisciplinary researchers.

Helix (gastropod)

10.2139/ssrn.3996122

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Moderate stability of a scissor double fluorescent triple helix molecular switch for the ultrasensitive biosensing of crop transgene

New Journal of Chemistry (2022)

Xiuling Chu Desong Zhu Min Liu Lingrang Kong Shiyun Ai

Schematic of the ultrasensitive biosensing of special genes. (I: traditional molecular beacon detection method; II: scissor DFTHMS; III: three cases of BHQ-1-TFO).

Schematic

Molecular beacon

10.1039/d2nj00647b

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Citations (4)

Improved Random Load Processing Algorithm Based on Kalman Filter

Advances in computer science research (2015)

Yong Gu Yun Zhu Desong Zhu

This paper first analyses the reasons of the disturbance signal emerging in the process of weighing, and then selects an improved algorithm based on Kalman filter method to deal with the raw data. After the analysis and comparing the new results with the static weighing data, we finally verify the accuracy of the new algorithm.

SIGNAL (programming language)

10.2991/isci-15.2015.36

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Protein binding protection in combination with DNA masking for sensitive and reliable transcription factor detection

Talanta (2018)

Xiaowen Xu Lei Wang Desong Zhu Yan Wang Wei Jiang

Transcription

10.1016/j.talanta.2018.04.047

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Sensitive detection of transcription factors using an Ag⁺-stabilized self-assembly triplex DNA molecular switch

Chemical Communications (2014)

Desong Zhu Jing Zhu Ye Zhu Lei Wang Wei Jiang

A simple, enzyme-free and sensitive new fluorescent strategy for detection of transcription factors was proposed based on a bifunctional Ag⁺-stabilized self-assembly triplex DNA molecular switch.

Transcription

10.1039/c4cc06205a

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Citations (31)

Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probe

Biosensors and Bioelectronics (2015)

Desong Zhu Lei Wang Xiaowen Xu Wei Jiang

G-quadruplex

10.1016/j.bios.2015.08.034

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Citations (32)

Fok I cleavage–inhibition strategy for the specific and accurate detection of transcription factors

Talanta (2015)

Ting Gao Lei Wang Jing Zhu Desong Zhu Wei Jiang

Transcription

Cleavage (geology)

HeLa

10.1016/j.talanta.2015.05.032

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Citations (5)

Manganese(II)-Enhanced Graphene Oxide Fluorescence Switch-Based Hybridization Chain Reaction for the Enzyme-Free and Sensitive Determination of Bacillus thuringiensis (Bt) in Transgenic Crops

Analytical Letters (2024)

Desong Zhu Xiuling Chu Chuan Zhang Shuai Chen Jianfeng Cao

Bacillus thuringiensis, as a special DNA sequence, has been introduced into crops for insect control. At the same time, the safety risk of transgenic crops has received wide concern. Herein, a simple, enzyme-free and sensitive novel nanosensor for the determination of Bacillus thuringiensis by manganese(II)-enhanced graphene oxide (GO) fluorescence switch (Mn(II)-GO-FS)-based hybridization chain reaction (HCR) signal amplification was developed. The high affinity of Mn(II) to oxygen-containing groups in double-stranded DNA and on the surface of GO was able to overcome the steric effect of rigid double chains and drive the efficient adsorption of HCR hairpin probes with sticky ends on GO. The efficient quenching of 6-carboxyfluorescein (FAM) labeled on the HCR hairpin probes guaranteed a low background. The Bacillus thuringiensis acted as a trigger to initiate a cascade of hybridization events between the two metastable HCR hairpin probes (H1 and H2) to polymerize into a nicked long double-helix structure (dsDNA). The products desorbed from the surface of GO and produced strong fluorescence. The results included a wide linear range (0.1 pM-50 nM) and a low detection limit (0.05 pM). This nanosensor was successfully applied to determine Bacillus thuringiensis in maize seeds with spiked recoveries between 97.5% and 103%. The Mn(II)-GO-FS had a simple design and was conveniently used for the determination of other special genes by changing the corresponding HCR hairpin probe sequence. Moreover, this novel nanosensor did not require harsh experimental conditions, operating in a neutral environment.

Bacillus thuringiensis

10.1080/00032719.2024.2350048

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Colocalization recognition-activated cascade signal amplification strategy for ultrasensitive detection of transcription factors

Biosensors and Bioelectronics (2016)

Desong Zhu Lei Wang Xiaowen Xu Wei Jiang

Colocalization

Transcription

10.1016/j.bios.2016.09.073

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Citations (11)