Hemostasis (HS) in 134 healthy subjects of over 20 years old was investigated. The cases with HS symptom were 45.5%, which increased with age. TXB2, 6-keto-PGF1 alpha and T/K ratio were measured by radioimmuno assay (RIA).elevation of TXB2 was more significant in the middle age and old age than in the young group (P < 0.01). But the level of 6-keto-PGF1 alpha in various age group didn't changed significantly; while the ratio between TXB2 and 6-keto-PGF1 alpha was more significant in the aged than in the young person (P < 0.01). The results revealed that there was hypercoagulable tendency with the increase of age, and it was correlated with TXB2 and 6-keto-PGF1 alpha. It is significant in theory and practice to prevent and cure the cardiovascular and cerebrovascular disease as well as HS with the traditional Chinese medicine.
Objective:To observe the expression of protein and DNA of postsynaptic density protein-95(PSD-95) in hippocampus of rats with morphine conditioned place preference(CPP) after environment induced reinstatement,and to evaluate the influence of PSD-95 on addiction memory.Methods:CPP rat model was established with morphine injection and reinstatement was induced by environment after extinction of CPP.The expression of PSD-95 protein and DNA in hippocampus of rats in reinstatement group of morphine CPP were observed,and was compared with those in extinction group of morphine CPP and control group.Results:The expression of PSD-95 in hippocampus was significantly decreased in rats in reinstatement and extinction group of morphine CPP compared with those in control group(P0.01),and it was signifi-cantly decreased in reinstatement group compared with those in extinction group(P0.01).Conclusion:The expression of PSD-95 in hippocampus is significantly decreased in rats in reinstatement and extinction group of morphine CPP.PSD-95 in hippocampus has take part or not in addict memory.
Objective To observe the protein expression of growth associated protein-43(GAP-43) in midbrain ventral tegmental area in morphine withdrawal rats at different time, and to evaluate the effect of GAP-43 on morphine withdrawal memory. Methods Rat models of morphine dependent 1 week, 2 weeks and 4 weeks were established by morphine hydrochloride intraperitoneal injection with increasing doses to establish natural withdrawal. The protein expression of GAP-43 in midbrain ventral tegmental area was observed by immunohistochemical staining and the results were analyzed by Image-Pro Plus 5.1 image analysis system. Results With prolongation of dependent time, the expression of GAP-43 was decreased then increased in midbrain ventral tegmental area. Conclusion GAP-43 could play a role in morphine withdrawal memory in midbrain ventral tegmental area.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)
