Event Abstract Back to Event Detection of Illicit Drugs in Fingerprints Using LC-MS and MALDI Kardazia Murry1, Kristen Mallory1, Ann-Elodie Robert1* and Murrell Godfrey1* 1 University of Mississippi, Department of Chemistry & Biochemistry, United States The drug composition of sweat within latent fingerprints was analyzed using two different analytical techniques, matrix assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI/TOF) and liquid chromatography mass spectrometry (LC-MS). Both instruments were used to determine the results of exogenous exposure with specific drugs and will later be used to analyze endogenous consumption. Ibuprofen, aspirin, acetaminophen, and caffeine were the drugs of choice in this study. Volunteers were instructed not to consume any of the listed drugs in the two week period prior to the collection of fingerprints. The volunteers washed their hands with water and soap to ensure the fingertips were clear of external contaminants. Volunteers provided both drug free fingerprint samples and fingerprints exposed to each drug powder. The prints were analyzed using MALDI/TOF and LC-MS. Different matrices and solvents were examined to determine the optimal sample preparation method that would yield the best results. Standards of each drug were used to determine limits of detection for each instrument and to generate standard curves with r-squared values greater than 0.95. Standard curves were used in the determination of exogenous drug concentrations. Future work will include analyzing the fingerprints of volunteers who have consumed the drugs of interest to determine a method for endogenous detection. The results of this experiment will help develop an accurate method for quantification of illicit drug components within latent fingerprints which can be applied to the field of forensic science. Acknowledgements I would like to thank Dr. Murrell Godfrey, Ann-Elodie Robert, and Caroline Spencer for providing guidance and expertise on this project. I would also like to thank Ms. Vinson, IMAGE, and the National Science Foundation for financial support. Keywords: Detection in Fingerprints, LC-MS, MALDI, Ibuprofen, exogenous, Acetaminophen Conference: National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference , Orlando, Florida, United States, 17 Sep - 20 Sep, 2018. Presentation Type: Poster Presentation Topic: Analytical Chemsitry Citation: Murry K, Mallory K, Robert A and Godfrey M (2019). Detection of Illicit Drugs in Fingerprints Using LC-MS and MALDI. Front. Chem. Conference Abstract: National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference . doi: 10.3389/conf.fchem.2018.01.00018 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 16 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Miss. Ann-Elodie Robert, University of Mississippi, Department of Chemistry & Biochemistry, Oxford, United States, arobert@go.olemiss.edu Dr. Murrell Godfrey, University of Mississippi, Department of Chemistry & Biochemistry, Oxford, United States, mgodfrey@olemiss.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kardazia Murry Kristen Mallory Ann-Elodie Robert Murrell Godfrey Google Kardazia Murry Kristen Mallory Ann-Elodie Robert Murrell Godfrey Google Scholar Kardazia Murry Kristen Mallory Ann-Elodie Robert Murrell Godfrey PubMed Kardazia Murry Kristen Mallory Ann-Elodie Robert Murrell Godfrey Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Event Abstract Back to Event DNA extraction from Modern Teeth Using Commercially Available DNA Extraction Kits Designed for Buccal Samples Ann-Elodie Robert1*, Caroline Spencer1, Deedra Hughes2, Tracey Dawson-Cruz3 and Murrell Godfrey1 1 University of Mississippi, United States 2 Mississippi Forensic Laboratory, United States 3 Virginia Commonwealth University, United States DNA can be extracted through different media, which includes but is not limited to blood, saliva, bones and even teeth. Most nuclear DNA and mitochondrial DNA (mtDNA) extraction kits are applied to blood or buccal cell samples and many of them are not capable of extracting DNA from ancient remains. The aim of this study was to compare two mtDNA extraction kits used to the extract DNA from teeth. Two extraction kits were examined 1) the QIAmp DNA Blood Mini Kit, used along with a protocol modified by the Dawson-Cruz lab at Virginia Commonwealth University, and 2) a Mitochondrial DNA Polymorphisms in Human Evolution Kit designed by Carolina Biological Supply Company. The first step of this study involved the extraction of DNA from teeth samples. Five unknown male teeth and five unknown female teeth were used in this study. These teeth were extracted within the last 2 years. Once the DNA extraction from the teeth samples were completed, amplification of both the nuclear and mtDNA was performed. Quantitation of the DNA extract was the last part of the experiment to be performed. The successful extraction of both nuclear and mtDNA was initially confirmed through gel electrophoresis and UV-Vis spectroscopy. DNA analysis was then performed using the ABI 7500 Genetic Analyzer to obtain a DNA profile and sex confirmation. Future goals of this study include applying extraction methods on teeth and other remains that range from decades to centuries old. Ultimately the goal is to design a method for extraction and analysis of DNA from ancient remains Keywords: forensic, teeth, DNA, extraction, Analytical, modified kits Conference: National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference , Orlando, Florida, United States, 17 Sep - 20 Sep, 2018. Presentation Type: Oral Presentation Topic: Biochemistry Citation: Robert A, Spencer C, Hughes D, Dawson-Cruz T and Godfrey M (2019). DNA extraction from Modern Teeth Using Commercially Available DNA Extraction Kits Designed for Buccal Samples. Front. Chem. Conference Abstract: National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference . doi: 10.3389/conf.fchem.2018.01.00020 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Ms. Ann-Elodie Robert, University of Mississippi, Oxford, United States, arobert@go.olemiss.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ann-Elodie Robert Caroline Spencer Deedra Hughes Tracey Dawson-Cruz Murrell Godfrey Google Ann-Elodie Robert Caroline Spencer Deedra Hughes Tracey Dawson-Cruz Murrell Godfrey Google Scholar Ann-Elodie Robert Caroline Spencer Deedra Hughes Tracey Dawson-Cruz Murrell Godfrey PubMed Ann-Elodie Robert Caroline Spencer Deedra Hughes Tracey Dawson-Cruz Murrell Godfrey Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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