Ling Dong

Dalian Medical University

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Lu Zhang

Southern University of Science and Technology

Cheng-Bo Hou

Beijing University of Chinese Medicine

Meng-ya Jin

Kai Dong

Changhai Hospital

Yuanming Luo

University of Iowa

Yan Yan

First Affiliated Hospital of Jiangxi Medical College

Xiaoqian Gao

Beijing Anzhen Hospital

Zhen Sun

Tongji Hospital

Na Xu

Guangdong Polytechnic of Science and Technology

Lina Wang

Tianjin Anding Hospital

Cooperative Institutions

Dalian Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Xi'an Jiaotong University

Peking University

Beijing University of Chinese Medicine

First Affiliated Hospital of Xi'an Jiaotong University

Chinese Academy of Agricultural Sciences

Chinese PLA General Hospital

Tianjin Medical University

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In vitro and in vivo application of pH-sensitive colon-targeting polysaccharide hydrogel used for ulcerative colitis therapy

Carbohydrate Polymers (2015)

Yu Cui You Ling Dong Kai Dong Wei Xu Yan Yan

10.1016/j.carbpol.2015.03.075

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Citations (60)

[Comparative proteomics study of different processing technology for pilose antler using iTRAQ technology coupled with 2D LC-MS].

PubMed (2015)

Meng-ya Jin Ling Dong Yuanming Luo Yu Li Mei Mo

This study was designed to use iTRAQ technology coupled with 2D LC-MS/MS to study the comparative proteomics of different processing technology for pilose antler. 1015 proteins were identified with 2D LC combined with MOLDI TOF/TOF mass spectrometry. Comparative analysis with Protein Pilot (Version 4.5) revealed that 87 proteins were changed (P ≤ 0.05, the ratio of > 1.50 or < 0.60 as the threshold selection of difference proteins), of which 24 were up regulated and 33 were down regulated in the traditional frying process (TFP) compared with the fresh pilose antler (P ≤ 0.05). 7 significant different proteins (P ≤ 0.001), most of these significantly changed proteins were found to be involved in calcium ion binding and ATP binding associated with human healthy. Freeze drying with protective agent (FDP) (Trehalose) can improve the content of significantly different proteins (P ≤ 0.001) including Collagen alpha-1 (XII) chain (COL12A1) and Collagen alpha-1 (II) chain (COL2A1). The significant function involves in platelets activating, maintenance of spermatogonium, and disorder expression in tumor cells. The functional annotation by Hierarchical clustering and GO (gene ontology) showed that the main molecule functions of the proteins significantly changed in these processes were involved in binding (52.7%), catalytic (25.3%), structural molecule and transporter (6.6%).

Antler

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[Comparative study on the protein maps of different parts of Cervus nippon antler by two-dimensional electrophoresis].

PubMed (2013)

Qian-Nan Ding Chunmei Wang Jun-dong Dai Ling Dong

To optimize the two-dimensional electrophoresis (2-DE) method for the proteome analysis of the Cervus nippon antler, and compare the protein maps of different parts of Cervus nippon antler.The total proteins of Cervus nippon antler were extracted by protein lysate containing 7 mol/L Urea, 4% CHAPS, 2 mol/L Thiourea, 65 mmol/L DTT, 1 mmol/L PMSF and 0.2% Bio-Lyte. The proteins were separated by immobilized pH 3 - 10 linear gradient (IPG), 7 cm strips as the first dimension. Isoelectric focusing conditions were optimized. 12% SDS-PAGE was used as the second dimension electrophoresis. The gels were stained with Coomassie brilliant blue and analyzed by PDQuest analysis software.The contents of total protein and the numbers of protein points of three different parts of Cervus nippon antler reduced gradually from the top to the bottom. Comparing three maps of different parts of Cervus nippon antler, there were 18 different protein points. Isoelectric point, molecular weight and gray value of each different protein point were calculated.An optimized two-dimensional electrophoresis method for the proteome analysis of the Cervus nippon antler is established. The 2-DE profiles of different parts of Cervus nippon antler exist obvious differences. The different protein points can be used as reference for Cervus nippon antler quality control and evaluation.

Cervus

Antler

Proteome

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Cyclic GMP-AMP synthase recognizes the physical features of DNA

Acta Pharmacologica Sinica (2024)

Ling Dong Yue-ru Hou Na Xu Xiaoqian Gao Zhen Sun

IRF3

10.1038/s41401-024-01369-7

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Citations (1)