V. C. Rayulu

Sri Venkateswara Veterinary University

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B. Sudhakara Reddy

Sri Venkateswara Veterinary University

S. Sivajothi

Sri Venkateswara Veterinary University

P. Malakondaiah

Sri Venkateswara Veterinary University

K. Nalini Kumari

Andhra University

P. M. Kondaiah

Sri Venkateswara Veterinary University

Ch. Srilatha

Professor Jayashankar Telangana State Agricultural University

V. Gnani Charitha

Sri Venkateswara Veterinary University

V. Vaikunta Rao

Sri Venkateswara Veterinary University

D. Sreenivasulu

Sri Venkateswara Veterinary University

Manigandan Lejeune

New York State College of Veterinary Medicine

Cooperative Institutions

Sri Venkateswara Veterinary University

Dr. NTR University of Health Sciences

University of Veterinary Medicine

Acharya N. G. Ranga Agricultural University

Kerala Veterinary and Animal Sciences University

Sri Venkateswara University

Tamil Nadu Veterinary and Animal Sciences University

P.V. Narsimha Rao Telangana Veterinary University

University of Veterinary and Animal Sciences

New York State College of Veterinary Medicine

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Prevalence of canine trypanosomiasis in certain areas of Andhra Pradesh

Journal of Parasitic Diseases (2013)

K. Lakshmi Prasad P. M. Kondaiah V. C. Rayulu Ch. Srilatha

Trypanosoma evansi

Blood smear

Blood film

10.1007/s12639-013-0326-1

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Citations (13)

Comparative evaluation of flotation techniques for the detection of soil borne parasites

Journal of Parasitic Diseases (2012)

V. Gnani Charitha V. C. Rayulu P. M. Kondaiah Ch. Srilatha

Ascaris

Soil test

10.1007/s12639-012-0176-2

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Citations (12)

Cutaneous lesions due to babesiosis in a dog

Comparative Clinical Pathology (2015)

B. Sudhakara Reddy S. Sivajothi V. C. Rayulu

Blood smear

Diminazene

Skin lesion

10.1007/s00580-015-2063-6

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Citations (0)

Clinical coccidiosis in adult cattle

Journal of Parasitic Diseases (2013)

B. Sudhakara Reddy S. Sivajothi V. C. Rayulu

Emaciation

Anorexia

Bloody diarrhea

Alimentary tract

10.1007/s12639-013-0395-1

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Citations (29)

Linguatula serrata Worm Infections in Dogs and Cats

V. Gnani Charitha C. Sreedevi V. C. Rayulu P. M. Kondaiah

Pentastomids, otherwise known as linguatulids or tongueworms, are a relatively neglected and enigmatic class of endoparasites that occupy a unique position among invertebrates. Linguatula serrata , a zoonotic and obligated endoparasite, is a member of class Pentastomida. The adult form of this parasite is found in the nasal airway, frontal sinus, and tympanic cavity of dogs and cats. Usually, infected dogs and cats show no clinical signs, however, massive infections can cause rhinitis and nasopharyngitis with chronic sneezing and/or coughing and purulent or blood-tinged nasal discharge. A varied range of mammals serve as intermediate hosts for L. serrata , including goats, sheep, cattle, rodents, rabbits, and camels. In livestock, most infections with nymphs remain asymptomatic, but there is some evidence, depending on the affected organs, that some clinical signs may develop. Humans may act as an accidental host in the life cycle of L. serrata and manifest with the nasopharyngeal, visceral, and ocular clinical forms. Diagnosis in dogs and other carnivores is confirmed upon the detection of adult worms by rhinoscopy or rhinotomy and by the detection of eggs in nasal discharge or feces. Diagnosis in human is usually incidental while investigating for other diseases. Macrocyclic lactones (milbemycin oxime, ivermectin) were found to be effective in treating canine cases. If not addressed timely, Linguatula infections in dogs and cats can pose serious public health problems and require a comprehensive control program and treatment strategies. Enlightening the public of the risks of consuming raw and undercooked viscera combined with a stringent assessment of visceral organs and lymph nodes in slaughterhouses helps keep a check on L. serrata infection in humans.

Sinus (botany)

Moxidectin

10.1002/9781394158256.ch5

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Histopathological Observations in Rabbits Experimentally Infected with Trypanosoma evansi

DOAJ (DOAJ: Directory of Open Access Journals) (2013)

S. Sivajothi V. C. Rayulu K. Sujatha B. Sudhakara Reddy P. Amaravathi

New Zealand white rabbits (n=6) were challenged with the South Indian local strain of Trypanosoma evansi. Each animal was infected with 5x105 trypanosomes subcutaneously. Animals were daily examined for the development of clinical signs using wet blood-films sampled from the ear veins. Clinically, intermittent pyrexia, undulating parasitaemia, anorexia and emaciation were predominant. Three months post infection, rabbits were sacrificed, detailed postmortem examination was carried out and representative tissue samples were fixed. Gross pathological changes including paleness of visceral organs, gelatinization of fat, congested and oedematous lungs,mucoid enteritis, hepatomegaly and splenomegaly were noticed. Histopathologically, internal organs elucidated clear changes consisted of severe hepatic fatty change, pulmonary congestion with thickened interstitial spaces and emphysema, degeneration of the renal epithelium associated with cystic tubular formation and congested red pulp.

Trypanosoma evansi

Trypanosoma vivax

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Monoclonal antibody based immunoassays for the detection of circulating antigens of Trypanosoma evansi in buffaloes

Italian Journal of Animal Science (2010)

V. C. Rayulu Ajit Singh S. S. Chaudhri

The present study reports the development and use of monoclonal antibody- based latex agglutination test (mAb-LAT) and antigen-detection ELISA (Ag-ELISA) for detection of Trypanosoma evansi infected buffaloes. MAb was produced for the first time against a surface antigen of an Indian isolate of T. evansi and used in development of mAb- LAT and Ag-ELISA for screening 285 buffalo sera samples collected from different regions of Haryana state in India during 2005 and 2006. For comparison, wet blood film (WBF) and microhematocrit technique (MHCT) were also used to detect the parasite in the blood samples from the same animals. Far more buffalo samples were declared positive by mAb-LAT and Ag-ELISA than those by WBF and MHCT. MAb-LAT was found to be a suitable fieldadaptable test for screening of large number of animals for T. evansi infections.

Trypanosoma evansi

10.4081/ijas.2007.s2.907

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Citations (17)

Development of Slide Enzyme Linked Immunosorbent Assay (SELISA) for Detection of Trypanosoma evansi Infection in Bovines

DOAJ (DOAJ: Directory of Open Access Journals) (2012)

S. Siva Jothi V. C. Rayulu B. Sudhakara Reddy

The Slide enzyme linked immunosorbent assay (SELISA) was standardized for detection of antibodies specific to Trypanosoma evansi and subsequently used for the screening of naturally infected bovine sera. A novel SELISA, a modification of the standard ELISA technique was used for the detection of antibodies against Trypanosoma evansi in bovines using positive and negative control sera. The test is based on immunostaining of the fixed whole Trypanosoma evansi organisms on microscopic glass slide, incubation with sera, antibovine IgG-HRPO conjugate and substrate Diaminobenzidine tetrahydrochloride (DAB). Finally the reaction was read under oil immersion of microscope. A total of 702 sera samples from bovines in Rayalaseema region of Andhra Pradesh were examined by SELISA and 192 were found positive for Trypanosoma evansi antibodies.

Trypanosoma evansi

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Intestinal coccidiosis infection in domestic rabbits

International Journal of Biological Research (2014)

S. Sivajothi B. Sudhakara Reddy V. C. Rayulu

The present study reports about the intestinal coccidiosis in a commercial rabbit farm. Seven New Zealand white rabbits were died in a commercial rabbit farm in Tirupati during the month of August. All the rabbits had signs of anorexia, diarrhea, dullness and weakness. Rabbits were necropsied and collected the intestinal contents for laboratory examination. Up on post mortem examination, hyperemic and oedematous intestinal mucosa was noticed. Oocysts of Eimeria spp. were detected in the faeces of all the rabbits. The collected oocysts were transferred into 2.5% aqueous potassium dichromate solution for sporulation. Faecal samples were examined by modified McMaster technique to determine oocyst counts which had ranged from 13,200 to 16,400. Keywords: Intestinal Coccidiosis, OPG, Rabbits, Sporulation.

Anorexia

Potassium dichromate

10.14419/ijbr.v2i2.2540

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Citations (9)

Diagnosis of bovine sarcocystosis by immunoflourescent antibody technique

Journal of Parasitic Diseases (2017)

Dasmabai Banothu Udayakumar Manchukonda V. C. Rayulu

Sarcocystosis

Antibody titer

Age groups

Infection rate

10.1007/s12639-017-0890-x

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Citations (3)