Ponciano D. Cruz

Southwestern Medical Center

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Kiyoshi Ariizumi

The University of Texas Southwestern Medical Center

Jin‐Sung Chung

Southwestern Medical Center

Paul R. Bergstresser

Kyushu University

Irene Dougherty

The University of Texas Southwestern Medical Center

Mizuki Tomihari

Osaka Metropolitan University

Hideo Akiyoshi

Osaka Metropolitan University

Jin-Sung Chung

The University of Texas Southwestern Medical Center

Makoto Bonkobara

Nippon Veterinary and Life Science University

Masato Kobayashi

Osaka University

Vijay Ramani

Washington University in St. Louis

Cooperative Institutions

The University of Texas Southwestern Medical Center

177

Southwestern Medical Center

University of Massachusetts Chan Medical School

American Academy of Dermatology

University of Pennsylvania

Icahn School of Medicine at Mount Sinai

Medical University of South Carolina

UConn Health

University of California, San Francisco

Harvard University

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Grenz Ray Therapy in the New Millennium: Still a Valid Treatment Option?

Dermatitis (2008)

Jennifer A. Warner Ponciano D. Cruz

Grenz ray therapy has declined in the United States during the past several decades with respect to availability, use, and resident training. However, it remains a simple, affordable, and effective option for managing several refractory dermatoses, especially psoriasis, hand eczema, and allergic contact dermatitis. Though high-dose grenz irradiation has been associated with the development of nonmelanoma skin cancers, grenz ray therapy is considered a safe treatment modality when administered according to the guidelines recommended herein. We review the value of grenz irradiation in contemporary dermatology.

Refractory (planetary science)

Treatment modality

10.2310/6620.2008.07078

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Citations (27)

Data from DC-HIL/Glycoprotein Nmb Promotes Growth of Melanoma in Mice by Inhibiting the Activation of Tumor-Reactive T Cells

Mizuki Tomihari Jin‐Sung Chung Hideo Akiyoshi Ponciano D. Cruz Kiyoshi Ariizumi

<div>AbstractDC-HIL/glycoprotein nmb (Gpnmb) expressed on antigen-presenting cells attenuates T-cell activation by binding to syndecan-4 (SD-4) on activated T cells. Because DC-HIL/Gpnmb is expressed abundantly by mouse and human melanoma lines, we posited that melanoma-associated DC-HIL/Gpnmb exerts similar inhibitory function on melanoma-reactive T cells. We generated small interfering RNA–transfected B16F10 melanoma cells to completely knock down DC-HIL/Gpnmb expression, with no alteration in cell morphology, melanin synthesis, or MHC class I expression. This knockdown had no effect on B16F10 proliferation in vitro or entry into the cell cycle following growth stimulation, but it markedly reduced the growth of these cells in vivo following their s.c. injection into syngeneic immunocompetent (but not immunodeficient) mice. This reduction in tumor growth was due most likely to an augmented capacity of DC-HIL–knocked down B16F10 cells (compared with controls) to activate melanoma-reactive T cells as documented in vitro and in mice. Whereas DC-HIL knockdown had no effect on susceptibility of melanoma to killing by cytotoxic T cells, blocking SD-4 function enhanced the reactivity of CD8+ T cells to melanoma-associated antigens on parental B16F10 cells. Using an assay examining the spread to the lung following i.v. injection, DC-HIL–knocked down cells produced lung foci at similar numbers compared with that produced by control cells, but the size of the former foci was significantly smaller than the latter. We conclude that DC-HIL/Gpnmb confers upon melanoma the ability to downregulate the activation of melanoma-reactive T cells, thereby allowing melanoma to evade immunologic recognition and destruction. As such, the DC-HIL/SD-4 pathway is a potentially useful target for antimelanoma immunotherapy. Cancer Res; 70(14); 5778–87. ©2010 AACR.</div>

10.1158/0008-5472.c.6500091

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Concurrent development of eruptive xanthogranulomas and hematologic malignancy: two case reports

Journal of the American Academy of Dermatology (2004)

Matthew Larson Chris Bandel Phillip J. Eichhorn Ponciano D. Cruz

Juvenile xanthogranuloma

10.1016/j.jaad.2003.08.019

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Citations (31)

Supplementary Fig. S1-S8 from Blocking Monocytic Myeloid-Derived Suppressor Cell Function via Anti-DC-HIL/GPNMB Antibody Restores the In Vitro Integrity of T Cells from Cancer Patients

Masato Kobayashi Jin‐Sung Chung Muhammad Shaalan Beg Yull Arriaga Udit Verma

Supplementary Fig. S1. Early stages CRC patients expressed very low DC-HIL expression on MDSCs. Supplementary Fig. S2. No effect of anti-CD14 mAb on T cell suppressor function of MDSCs. Supplementary Fig. S3. DC-HILneg MC38 and CT26 mouse colon cancer cell lines exhibit high response to anti-DC-HIL treatment. Supplementary Fig. S4. MDSCs in spleen and draining lymph nodes did not express DC-HIL. Supplementary Fig. S5. Combination treatment of anti-DC-HIL/anti-PDL1 mAb did not produce synergistic anti-tumor effects to MC38 colon cancer. Supplementary Fig. S6. Changes in health conditions of mice treated with anti-DC-HIL mAb. Supplementary Fig. S7. Representative FACS data showing frequency of immune cells in the tumor microenvironment of mice treated with mAb. Supplementary Fig. S8. Blood granulocytic MDSC of CRC patients expressed low-levels of DC-HIL and were not immunosuppressive in ex vivo cultures.

Myeloid-derived Suppressor Cell

Ex vivo

10.1158/1078-0432.22474694.v1

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Supplementary Table S1 -S5 from Blocking Monocytic Myeloid-Derived Suppressor Cell Function via Anti-DC-HIL/GPNMB Antibody Restores the In Vitro Integrity of T Cells from Cancer Patients

Masato Kobayashi Jin‐Sung Chung Muhammad Shaalan Beg Yull Arriaga Udit Verma

Supplementary Table S1. Summary of MDSC analysis of metastatic cancer patents. aAll cancer patients are diagnosed as stage III or IV. Supplementary Table S2. Correlation of variables and expansion of DC-HIL+ MDSCs among colorectal cancer patients. Supplementary Table S3. % DC-HIL+ MDSC/PBMC in subgroups with vs. without radiotherapy. Supplementary Table S4. Reversing effects of anti-DC-HIL vs. anti-PDL1 mAb on MDSC suppressor function between two different treatments. Supplementary Table S5. DC-HIL and PDL1 expression in colorectal cancer tissues.

Myeloid-derived Suppressor Cell

10.1158/1078-0432.22474691.v1

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Data from DC-HIL/Gpnmb Is a Negative Regulator of Tumor Response to Immune Checkpoint Inhibitors

Jin‐Sung Chung Vijay Ramani Masato Kobayashi Farjana Fattah Vinita Popat

<div>AbstractPurpose:Immune checkpoint inhibitors (ICI) benefit only a minority of treated patients with cancer. Identification of biomarkers distinguishing responders and nonresponders will improve management of patients with cancer. Because the DC-HIL checkpoint differs from the PD1 pathway in expression and inhibitory mechanisms, we examined whether DC-HIL expression regulates ICI responsiveness.Experimental Design:Plasma samples were collected from patients with advanced non–small cell lung carcinoma (NSCLC) (n = 76) at baseline and/or follow-up after ICI monotherapy. Blood-soluble DC-HIL (sDC-HIL) was determined and analyzed for correlation with the early tumor response. To study the mechanisms, we measured effect of anti-DC-HIL versus anti-PDL1 mAb on growth of mouse tumor cells in experimentally metastatic lung. Influence of DC-HIL to anti-PDL1 treatment was assessed by changes in tumor response after deletion of host-DC-HIL gene, injection of DC-HIL–expressing myeloid-derived suppressor cells (MDSC), or induction of sDC-HIL expression.Results:Nonresponders expressed significantly higher levels of baseline sDC-HIL levels than responders. Among patients (n = 28) for fluctuation with time, nonresponders (14/15 cases) showed increasing or persistently elevated levels. Responders (12/13) had decreasing or persistently low levels. Among various tumors, B16 melanoma exhibited resistance to anti-PDL1 but responded to anti-DC-HIL mAb. Using B16 melanoma and LL2 lung cancer, we showed that deletion of host-derived DC-HIL expression converted the resistant tumor to one responsive to anti-PDL1 mAb. The responsive state was reversed by infusion of DC-HIL+MDSC or induction of sDC-HIL expression.Conclusions:sDC-HIL in the blood and probably DC-HIL receptor expressed by MDSC play an important role in regulating response to ICI in advanced NSCLC.</div>

Lewis lung carcinoma

10.1158/1078-0432.c.6529857.v1

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Supplementary Figure 3 from DC-HIL/Glycoprotein Nmb Promotes Growth of Melanoma in Mice by Inhibiting the Activation of Tumor-Reactive T Cells

Mizuki Tomihari Jin‐Sung Chung Hideo Akiyoshi Ponciano D. Cruz Kiyoshi Ariizumi

Supplementary Figure 3 from DC-HIL/Glycoprotein Nmb Promotes Growth of Melanoma in Mice by Inhibiting the Activation of Tumor-Reactive T Cells

10.1158/0008-5472.22382070

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Photodermatitis for the Allergist

Current Allergy and Asthma Reports (2017)

Samuel L. Coffin Jake E. Turrentine Ponciano D. Cruz

10.1007/s11882-017-0705-2

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Citations (8)

Spleen dendritic cells exhibit altered morphology and increased allostimulatory capacity after short‐term culture*

Experimental Dermatology (1992)

Giampiero Girolomoni Giovanna Zambruno V. Manca Paul R. Bergstresser Alberto Giannetti

Abstract la‐bearing dendritic cells (DC) are a class of bone marrow‐derived antigen‐presenting cells that appear to possess an increased capacity to stimulate resting T lymphocytes. DC from different tissues share several morphologic, phenotypic and functional attributes. For example, freshly isolated DC from spleen resemble phenotypically and functionally freshly isolated Langerhans cells (LC) from epidermis; in addition, during short‐term culture both DC and LC undergo several parallel changes including modifications affecting phenotype, capacity to present protein antigens, and ability to route surface la molecules into intracellular acidic compartments (J Immunol 1990: 145: 2820–2826). In the present study we show, using immunoelectron microscopy with anti‐la and anti‐33Dl monoclonal antibodies, freshly isolated DC in suspension to have a smooth cell surface with few and short cytoplasmic projections. By contrast, cultured DC display conspicuous bulbous cytoplasmic protrusions. In addition, spleen DC following culture for 24–48 hours exhibit an increased ability to stimulated allogeneic T lymphocytes in the primary mixed leukocyte reaction. These changes, similar to those described for freshly isolated and cultured LC respectively, further substantiate the close relationship between DC and LC.

Immunoelectron microscopy

10.1111/j.1600-0625.1992.tb00004.x

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Citations (6)

Expression of the Tumor Necrosis Factor Gene by Dermal Fibroblasts in Response to Ultraviolet Irradiation or Lipopolysaccharide

Journal of Investigative Dermatology (1995)

S de Kossodo Ponciano D. Cruz Irene Dougherty Patricia A. Thompson Maria Silva-Valdez

Epidermis (zoology)

10.1111/1523-1747.ep12665361

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Citations (41)