We have isolated a novel 40 amino acid neuropeptide, designated manserin, from the rat. Manserin is derived from secretogranin II (SgII), a member of granin acidic secretory protein family by proteolytic processing, as previously reported secretoneurin and EM66. Immunohistochemical analysis using anti-manserin antibody revealed that manserin localized in the endocrine cells of the pituitary anterior lobe, but not in the posterior lobe. Interestingly, manserin never co-localized with ACTH in the anterior pituitary, which is in contrast with SgII, suggesting specific immunoreactivity of the antiserum against manserin. Manserin immunostaining was also observed in the neuronal cells of several hypothalamic nuclei and the neurons in the median eminence. These results suggest that manserin exerts a specific role in the neuroendocrine system.

Median eminence

Immunostaining

Colocalization

Posterior pituitary

Enteroendocrine cell

10.1097/01.wnr.0000127634.38052.84

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Citations (33)

The cellular localization of 5-HT2A receptors in the spinal cord and spinal ganglia of the adult rat

Brain Research (1998)

Toru Maeshima Ryuzo Ito Shun Hamada Kouji Senzaki Kayoko Hamaguchi‐Hamada

GDF7

Dorsal root ganglion

Anterior Horn Cell

Cell bodies

10.1016/s0006-8993(98)00360-6

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Citations (63)

The central distribution pattern of primary afferent fibers innervating the thigh muscle posterior iliotibialis in the chicken.

PubMed (1999)

Toru Maeshima Ito R Masakatsu Matsukawa M Usuba Nobuo Okado

The anatomical relationship between motoneuron dendrites and primary afferent fibers innervating the same muscle were examined by using a modified cholera toxin tracing method. The cholera toxin B subunit conjugated with latex beads was injected into the posterior iliotibial muscle (PIT) of chicken. Both motoneuron dendrites and primary afferent fibers were intensely labeled. Labeled primary afferent fibers innervating the PIT were mainly located in laminae I (ventral part), V (lateral part), VI, VII (except ventromedial part) and lateral motor column (LMC, lamina IX) of the lumbar spinal cord. Numerous labeled primary afferent fibers were observed in proximity to the motoneuron pool of the PIT located in the dorsolateral region of the LMC; primary afferent fibers were closely apposed to somatic profiles of the PIT motoneurons.

Cholera toxin

Source

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Citations (1)

Aplications of cholera toxin subunit B for the labeling of primary afferents in the spinal cord

Neuroscience Research Supplements (1991)

Toru Maeshima Ryuzo Ito Kunio Kohno Nobuo Okado

Cholera toxin

Primary (astronomy)

10.1016/s0921-8696(06)80462-7

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Laser's effect on bone and cartilage change induced by joint immobilization: An experiment with animal model

Lasers in Surgery and Medicine (1997)

Masami Akai Mariko Usuba Toru Maeshima Yoshio Shirasaki Shozo Yasuoka

Objective Influence of low-level (810nm, Ga-Al-As semi-conductor) laser on bone and cartilage during joint immobilization was examined with rats' knee model. Materials and Methods The hind limbs of 42 young Wistar rats were operated on in order to immobilize the knee joint. One week after operation they were assigned to three groups; irradiance 3.9W/cm2, 5.8W/cm2, and sham treatment. After 6 times of treatment for another 2 weeks both hind legs were prepared for 1) indentation of the articular surface of the knee (stiffness and loss tangent), and for 2) dual energy X-ray absorptiometry (bone mineral density) of the focused regions. Results and Conclusions The indentation test revealed preservation of articular cartilage stiffness with 3.9 and 5.8W/cm2 therapy. Soft laser treatment has a possibility for prevention of biomechanical changes by immobilization. Lasers Surg. Med. 21:480–484, 1997. © 1997 Wiley-Liss, Inc.

Hindlimb

Indentation

10.1002/(sici)1096-9101(1997)21:5<480::aid-lsm10>3.3.co;2-q

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Citations (1)

The authors respond

Archives of Physical Medicine and Rehabilitation (2006)

Mariko Usuba Yutaka Miyanaga Shumpei Miyakawa Toru Maeshima Yoshio Shirasaki

Goniometer

Wonder

10.1016/j.apmr.2006.10.010

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