David J. Volsky

Icahn School of Medicine at Mount Sinai

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Mary Jane Potash

Icahn School of Medicine at Mount Sinai

Wei Chao

Icahn School of Medicine at Mount Sinai

Eran Hadas

Icahn School of Medicine at Mount Sinai

Galina Bentsman

St. Luke's-Roosevelt Hospital Center

Alejandra Borjabad

Icahn School of Medicine at Mount Sinai

Faruk Sinangil

Global Solutions for Infectious Diseases

Paul B. Fisher

Virginia Commonwealth University

Jennifer Kelschenbach

Icahn School of Medicine at Mount Sinai

Zao-zhong Su

Virginia Commonwealth University

Barbara Volsky

New York University

Cooperative Institutions

Columbia University

University of Nebraska Medical Center

St. Luke's-Roosevelt Hospital Center

Johns Hopkins University

University of California, San Diego

Johns Hopkins Medicine

Icahn School of Medicine at Mount Sinai

Columbia University Irving Medical Center

Harvard University

National Institutes of Health

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EcoHIV Infection of Primary Murine Brain Cell Cultures to Model HIV Replication and Neuropathogenesis

Viruses (2024)

Boe‐Hyun Kim Wei Chao Eran Hadas Alejandra Borjabad Mary Jane Potash

EcoHIV is a chimeric HIV that replicates in mice in CD4+ T cells, macrophages, and microglia (but not in neurons), causing lasting neurocognitive impairment resembling neurocognitive disease in people living with HIV. The present study was designed to develop EcoHIV-susceptible primary mouse brain cultures to investigate the indirect effects of HIV infection on neuronal integrity.

10.3390/v16050693

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Citations (4)

Additional file 2: Figure S2. of Oncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicity

Shamsudheen Moidunny Marco Matos Evelyn M. Wesseling Santanu Banerjee David J. Volsky

Excitotoxicity

Neurotoxicity

Oncostatin M

Source

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Citations (0)

RECONSTITUTION OF THE ERYTHROCYTE ANION TRANSPORT SYSTEM: IN VITRO AND IN VIVO APPROACHES*

Annals of the New York Academy of Sciences (1980)

Z. Ioav Cabantchik David J. Volsky H. Ginsburg Abraham Loyter

Hebrews

10.1111/j.1749-6632.1980.tb47189.x

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Citations (30)

ANTIBODIES TO AIDS-ASSOCIATED RETROVIRUS (HTLV-III/LAV) IN DRUG ADDICTS FROM VIZCAYA, NORTHERN SPAIN

Aids Research (1986)

Fernando Merino B Esparza Javier Aizpiri José Luis Fernández L. de Masdelval

Serum samples from 313 asymptomatic intravenous (IV) drug users from Bilbao (Vizcaya, Vasque Country, Spain) were tested for antibodies to HTLV-III/LAV virus, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS). Viral antibodies were assayed by ELISA test. 41.9% of the sera gave positive reactions. No seropositivity was detected among 22 normal blood donors, 58 chronic alcoholics, or 20 members of the Drug Control Center personnel. Virus specific reactions were confirmed by indirect immunofluorescence using an HTLV-III/LAV producer cell line, and by Western blotting. 55% of the ELISA-positive sera were also positive in Western blot assay. No differences in seropositivity by age or sex were observed but it increased with the period of parenteral drug use. Presence of antibody statistically correlated with the frequency of syringe sharing, confirming the transmission of viral infection by blood products. Altered T4/T8 ratios and lower number of T4 positive lymphocytes were detected among HTLV-III/LAV positive drug addicts.

Asymptomatic carrier

Immunofluorescence

Drug addict

10.1089/aid.1.1986.2.133

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Citations (5)

Innate immune responses reverse HIV cognitive disease in mice: Profile by RNAseq in the brain

Virology (2023)

Alejandra Borjabad Baojun Dong Wei Chao David J. Volsky Mary Jane Potash

Antiretroviral therapy controls immunodeficiency in people with HIV but many develop mild neurocognitive disorder. Here we investigated HIV brain disease by infecting mice with the chimeric HIV, EcoHIV, and probing changes in brain gene expression during infection and reversal with polyinosinic-polycytidylic acid (poly I:C). EcoHIV-infected C57BL/6 mice were treated with poly I:C and monitored by assay of learning in radial arm water maze, RNAseq of striatum, and QPCR of virus burden and brain transcripts. Poly I:C reversed EcoHIV-associated cognitive impairment and reduced virus burden. Major pathways downregulated by infection involved neuronal function, these transcriptional changes were normalized by poly I:C treatment. Innate immune responses were the major pathways induced in EcoHIV-infected, poly I:C treated mice. Our findings provide a framework to identify brain cell genes dysregulated by HIV infection and identify a set of innate immune response genes that can block systemic infection and its associated dysfunction in the brain.

Neurocognitive

10.1016/j.virol.2023.109917

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Citations (2)

Activation of NF-κB by R5 and X4 Human Immunodeficiency Virus Type 1 Induces Macrophage Inflammatory Protein 1α and Tumor Necrosis Factor Alpha in Macrophages

Journal of Virology (2002)

Wonkyu Choe David J. Volsky Mary Jane Potash

ABSTRACT NF-κB is a transcriptional activator that often regulates inflammatory responses. We demonstrate that human immunodeficiency virus type 1 activates nuclear localization of NF-κB in macrophages in a manner dependent upon virus strain but independent of virus replication. Through the use of an inhibitor, NF-κB activation was found to be responsible for the cytokine and chemokine induction that we recently reported.

Macrophage inflammatory protein

Proinflammatory cytokine

10.1128/jvi.76.10.5274-5277.2002

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Citations (25)

Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV

Journal of Neuroimmune Pharmacology (2011)

Jennifer Kelschenbach Manisha Saini Eran Hadas Chaojiang Gu Wei Chao

Superinfection

Adoptive Cell Transfer

Cellular immunity

10.1007/s11481-011-9316-1

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Citations (36)

Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges

PLoS Pathogens (2011)

Eung‐Jun Im Jessie P. Hong Yaowaluck Maprang Roshorm Anne Bridgeman Sven Létourneau

Immunodominance in T cell responses to complex antigens like viruses is still incompletely understood. Some data indicate that the dominant responses to viruses are not necessarily the most protective, while other data imply that dominant responses are the most important. The issue is of considerable importance to the rational design of vaccines, particularly against variable escaping viruses like human immunodeficiency virus type 1 and hepatitis C virus. Here, we showed that sequential inactivation of dominant epitopes up-ranks the remaining subdominant determinants. Importantly, we demonstrated that subdominant epitopes can induce robust responses and protect against whole viruses if they are allowed at least once in the vaccination regimen to locally or temporally dominate T cell induction. Therefore, refocusing T cell immune responses away from highly variable determinants recognized during natural virus infection towards subdominant, but conserved regions is possible and merits evaluation in humans.

Subdominant

Immunodominance

Original antigenic sin

10.1371/journal.ppat.1002041

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Citations (64)

CD4 Confers Susceptibility to Human Immunodeficiency Virus Type 1 Infection in a Rat Fibroblast Cell Line

AIDS Research and Human Retroviruses (1996)

Yaffa Mizrachi Arye Rubinstein Mala Golodner Koji Sakai David J. Volsky

A new model system is delineated that will enable study of CD4 cofactors and gp120 binding proteins other than CD4. We have previously described a nontransformed rat fibroblast cell line that can efficiently produce HIV-1 upon transfection with an HIV-1 infectious clone, in contrast to other nonhuman mammalian cell lines tested. In the present study we analyzed the susceptibility to HIV-1 infection of Rat2 cells expressing the human CD4 protein. We have used the mammalian expression vector pKS286, in which HIV-1 LTR drives the expression of the CD4 gene. We show that the Rat2 cell line, expressing the human CD4 (Rat2/CD4), is susceptible to fusion with and infection by HIV-1. The virus produced by the Rat2/CD4 cells was infectious. CD4 expression in the Rat2/CD4 was down-regulated over time, similarly to HIV-1 expression in HIV-1-transfected Rat2 cells. Transfection of the Rat2/CD4 cells with a tat expression vector reestablished the CD4 expression on the surface of those cells, as expected. We conclude that the expression of a gp120 binding protein on the Rat2 cells' surface suffices to render the Rat2 cells susceptible to HIV-1 infection.

clone (Java method)

10.1089/aid.1996.12.1315

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Citations (1)

Molecular differences in the genomes of HTLV III/LAV viruses isolated on two different occasions from a patient with lymphadenopathy

Annales de l Institut Pasteur Virologie (1986)

Kyoko Sakai D Casareale Joseph Sonnabend David J. Volsky

10.1016/s0769-2617(86)80222-2

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Citations (10)