Nicolle L. Barbieri

University of Georgia

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Catherine M. Logue

University of Georgia

Lisa K. Nolan

University of Georgia

Daniel W. Nielsen

Oak Ridge Institute for Science and Education

Aline Luísa de Oliveira

University of Georgia

Darby M. Newman

University of Georgia

Heather K. Allen

National Animal Disease Center

Nicole Ricker

University of Guelph

Meaghan M. Young

University of Georgia

Yvonne Wannemuehler

Iowa State University

Miliane Moreira Soares de Souza

Universidade Federal Rural do Rio de Janeiro

Cooperative Institutions

University of Georgia

Iowa State University

Universidade Federal do Rio Grande do Sul

Universidade Federal Rural do Rio de Janeiro

Universidade Positivo

U.S. National Poultry Research Center

Ames National Laboratory

Georgia College & State University

Mississippi State University

Agricultural Research Service

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Apicoectomy after conventional endodontic treatment failure: case report

RSBO Revista Sul-Brasileira de Odontologia (2013)

Lorena Oliveira Pedroche Nicolle L. Barbieri Flávia Sens Fagundes Tomazinho Luciene Miranda Ulbrich Denise Piotto Leonardi

Resumen en: Introduction: Paraendodontic surgery is a safe and adequate alternative when teeth are not responding to conventional treatment and endodontic re-treatme...

Apicoectomy

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Accuracy of PCR universal primer for methicillin-resistant Staphylococcus and comparison of different phenotypic screening assays

Brazilian Journal of Microbiology (2019)

Dayanne Araújo de Melo Bianca da Silva Soares Cássia Couto da Motta Felipe C. Dubenczuck Nicolle L. Barbieri

Primer (cosmetics)

SCCmec

10.1007/s42770-019-00171-6

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INVESTIGAÇÃO DA PRESENÇA DE PARASITAS EM CRIANÇAS CARENTES RESIDENTES EM UM LOTEAMENTO DE PORTO ALEGRE

Marilise Brittes Rott Roberta Souza dos Reis Amanda Bencke Geórgia Lazzari Artuso Nicolle L. Barbieri

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Verificação da ativação da caspase 3/7 em macrófagos aviários HD11 por cepas patogênicas aviárias de Escherichia coli

Nicolle L. Barbieri Francine Johansson Azeredo

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Laser Capture Microdissection, Culture Analysis, and Bacterial Sequencing to Evaluate the Microbiota of Focal Duodenal Necrosis in Egg Layers

Avian Diseases (2023)

Yu-Yang Tsai Monique França Alvin C. Camus Lisa Stabler Nicolle L. Barbieri

Focal duodenal necrosis (FDN) is a common intestinal disease of table egg layers. In this research we aimed to identify the bacteria commonly found in FDN lesions as seen with histopathological analysis. Fifty-nine ethanol-fixed duodenum samples were collected from egg layers on eight FDN-affected farms, and 42 samples had typical FDN lesions. Excision of bacteria-containing lesions using laser capture microdissection was performed, followed by 16S rRNA gene sequencing of extracted DNA for bacterial identification. Bacterial sequencing analysis revealed no consistent bacterial species identified from samples with FDN. However, analysis of the relative phylum abundance revealed differences in the duodenal microbiota between layers with FDN and healthy birds. There were differences in the abundance of Proteobacteria, Firmicutes, and Actinobacteria between FDN-positive and FDN-negative control samples compatible with intestinal dysbiosis. In addition, 10 duodenal samples with FDN lesions were collected for bacteriological analysis, yielding 47 colonies on tryptone soy agar, MacConkey agar, and blood agar plates. Using 16S rRNA gene PCR, 39/47 (53.8%) colonies were identified as Escherichia coli. PCR for E. coli virulence genes identified 21/39 (53.8%) E. coli isolates as avian pathogenic E. coli-like. PCR analysis for 19 E. coli virulence genes associated with intestinal disease strains including inflammatory bowel disease found 11/39 (28.2%) isolates containing more than 10 of these virulence genes. In conclusion, FDN appears to be a multifactorial inflammatory intestinal disease associated with intestinal dysbiosis, and Gram-negative bacteria including E. coli may contribute to the pathogenesis of this disease.Microdisección por captura láser, análisis de cultivos y secuenciación bacteriana para evaluar la microbiota de la necrosis duodenal focal en aves de postura de huevo comercial. La necrosis duodenal focal (FDN) es una enfermedad intestinal común en las gallinas de postura de huevo comercial. En esta investigación, el objetivo fue identificar las bacterias que se encuentran comúnmente en las lesiones provocadas por la necrosis duodenal focal tal como se aprecian con el análisis histopatológico. Se recolectaron 59 muestras de duodeno fijadas con etanol de gallinas de postura de ocho granjas afectadas por necrosis duodenal focal, y 42 muestras tenían lesiones típicas de dicha enfermedad. Se realizó la escisión de las lesiones que contenían bacterias mediante microdisección por captura láser, seguida de la secuenciación del gene 16S rRNA del ADN extraído para la identificación bacteriana. El análisis de secuenciación bacteriana no reveló especies bacterianas consistentes identificadas a partir de muestras con necrosis duodenal focal. Sin embargo, el análisis de la abundancia relativa del phylum reveló diferencias en el microbiota duodenal entre gallinas de postura con necrosis duodenal focal y aves sanas. Hubo diferencias en la abundancia de Proteobacteria, Firmicutes y Actinobacteria entre las muestras controles positivas y negativas para la necrosis duodenal focal compatibles con disbiosis intestinal. Además, se recolectaron 10 muestras duodenales con lesiones de la necrosis duodenal focal para análisis bacteriológico, lo que produjo 47 colonias en placas de agar triptona soya, agar MacConkey y agar sangre. Utilizando un método de PCR para el gene 16S rRNA, 39/47 (53.8 %) colonias se identificaron como Escherichia coli. El método de PCR para genes de virulencia de E. coli identificó 21/39 (53.8 %) aislados de E. coli como similares a E. coli patogénica aviar. El análisis de PCR para 19 genes de virulencia de E. coli asociados con cepas que provocan enfermedades intestinales, incluida la enfermedad inflamatoria intestinal, detectó 11/39 (28.2 %) aislados que contenían más de 10 de estos genes de virulencia. En conclusión, la necrosis duodenal focal parece ser una enfermedad intestinal inflamatoria multifactorial asociada con disbiosis intestinal, y las bacterias Gramnegativas, incluida E. coli, pueden contribuir a la patogenia de esta enfermedad.

Laser capture microdissection

Dysbiosis

10.1637/aviandiseases-d-22-00088

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Evaluation of immunogenicity and efficacy of the enterobactin conjugate vaccine in protecting chickens from colibacillosis

Vaccine (2022)

Huiwen Wang Liu Cao Catherine M. Logue Nicolle L. Barbieri Lisa K. Nolan

Pathogenic Escherichia coli

Enterobactin

Booster dose

Inactivated vaccine

10.1016/j.vaccine.2022.12.057

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Antimicrobial resistance profile of Escherichia coli isolated from poultry litter

Poultry Science (2022)

M.J. Khong Ashlyn M. Snyder Anna K. Magnaterra Meaghan M. Young Nicolle L. Barbieri

Antimicrobial resistance is a threat to animal and human health. As a commensal and zoonotic bacterium, Escherichia coli has the potential to be a pathogenic source of antimicrobial resistance. The purpose of this study aimed to investigate the antimicrobial resistance profile of E. coli isolated from litter collected from pens in a broiler chicken experiment. E. coli was isolated from litter samples (n = 68 isolates) of 16 pens housing broilers to d 53 of age. Resistance to 10 antimicrobials was observed by disc diffusion. The presence of 23 antimicrobial and heavy metal resistance genes, O serogroups, and avian pathogenic E. coli (APEC-like) minimal predictor genes were identified through PCR. E. coli isolates presented the greatest resistance to cephalothin (54.4%), tetracycline (27.9%), streptomycin (29.4%), ampicillin (20.6%), colistin (13.2%), sulphonamides (8.8%), and imipenem (1.5%). Multidrug resistance to at least 3 antimicrobials was observed in 22.1% of isolates. The identified O-types of the E. coli isolates were O15, O75, O78, and O91. There was a greater likelihood that the genes groEL, aph(3)IA, silP, sull, aadA, qacEdelta1, iroN, ompTp, and hlyF were present in isolates that exhibited ampicillin resistance (P ≤ 0.05). There was a greater likelihood that the groEL gene was present in isolates resistant to ampicillin, colistin, tetracycline, sulphonamides, or cephalothin (P ≤ 0.05). Further characterizing E. coli antimicrobial resistance is essential and aids in developing effective solutions, thereby furthering the One Health objective.

Colistin

Pathogenic Escherichia coli

10.1016/j.psj.2022.102305

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Additional file 3 of Outer membrane protein A (OmpA) of extraintestinal pathogenic Escherichia coli

Figshare (2020)

Daniel A. Nielsen Nicole Ricker Nicolle L. Barbieri Heather K. Allen Lisa K. Nolan

Additional file 3. Sequences for OmpA analyzed including the strain ID, ompA sequence, non dimer and dimer regions, polymorphisms identified, sub pathotype of the strains, polymorphism pattern ID, and phylogenetic group.

Pathogenic Escherichia coli

10.6084/m9.figshare.11785200

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Characterizing the Type 6 Secretion System (T6SS) and its role in the virulence of avian pathogenic Escherichia coli strain APECO18

PeerJ (2021)

Aline Luísa de Oliveira Nicolle L. Barbieri Darby M. Newman Meaghan M. Young Lisa K. Nolan

Avian pathogenic E. coli is the causative agent of extra-intestinal infections in birds known as colibacillosis, which can manifest as localized or systemic infections. The disease affects all stages of poultry production, resulting in economic losses that occur due to morbidity, carcass condemnation and increased mortality of the birds. APEC strains have a diverse virulence trait repertoire, which includes virulence factors involved in adherence to and invasion of the host cells, serum resistance factors, and toxins. However, the pathogenesis of APEC infections remains to be fully elucidated. The Type 6 secretion (T6SS) system has recently gained attention due to its role in the infection process and protection of bacteria from host defenses in human and animal pathogens. Previous work has shown that T6SS components are involved in the adherence to and invasion of host cells, as well as in the formation of biofilm, and intramacrophage bacterial replication. Here, we analyzed the frequency of T6SS genes hcp, impK, evpB, vasK and icmF in a collection of APEC strains and their potential role in virulence-associated phenotypes of APECO18. The T6SS genes were found to be significantly more prevalent in APEC than in fecal E. coli isolates from healthy birds. Expression of T6SS genes was analyzed in culture media and upon contact with host cells. Mutants were generated for hcp, impK, evpB, and icmF and characterized for their impact on virulence-associated phenotypes, including adherence to and invasion of host model cells, and resistance to predation by Dictyostelium discoideum. Deletion of the aforementioned genes did not significantly affect adherence and invasion capabilities of APECO18. Deletion of hcp reduced resistance of APECO18 to predation by D. discoideum, suggesting that T6SS is involved in the virulence of APECO18.

Pathogenic Escherichia coli

Virulence factor

10.7717/peerj.12631

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Adesão de uma cepa patogênica aviária de Escherichia coli a células epiteliais

Nicolle L. Barbieri Michele Bastiani Roberta Souza dos Reis Francine Johansson Azeredo

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