Han Chang Sohn

Jeju National University

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Jehee Lee

Jeju National University

Sang Phil Shin

Kongju National University

Chang Nam Jin

Jeju National University

Bong Jo Kang

National Fisheries Research and Development Institute

Jee Youn Hwang

National Fisheries Research and Development Institute

Seong Don Hwang

Korea Maritime and Ocean University

Seongdo Lee

National Pension Service

SP Shin

Jeju National University

Chang-Nam Jin

Jeju National University

Mun Gyeong Kwon

National Pension Service

Cooperative Institutions

Jeju National University

National Fisheries Research and Development Institute

National Pension Service

National Fisheries Institute

Korea Fisheries Resources Agency

Research & Development Institute

Tokyo University of Marine Science and Technology

Aquatic Animal Health Research Laboratory

The University of Tokyo

Seoul National University

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Kudoa ogawai (Myxosporea: Kudoidae) Infection in Cultured Olive Flounder Paralichthys olivaceus

Korean Journal of Parasitology (2019)

Sang Phil Shin Chang Nam Jin Han Chang Sohn Jehee Lee

Since Kudoa septempuntata was identified as a causative agent of food poisoning associated with raw olive flounder Paralichthys olivaceus, interest and concern regarding the parasite have increased. However, there have been no investigations or reports of other Kudoa species infecting the fish (except for K. paralichthys, which infects the brain) in Korea. We found cysts filled with myxospores of Kudoa species in muscles of cultured olive flounder specimens and identified these to the species level. Mature spores were quadrate, measuring 8.7±0.5 μm in length, 9.2±0.4 μm in thickness, and 12.9±0.6 μm in width. The spores containing 4 polar capsules had a length of 2.1±0.2 μm and a width of 1.8±0.3 μm. The partial 18S and 28S rDNA of isolates showed 99-100% similarities with K. ogawai. Using these morphological and molecular analyses, the species was identified as K. ogawai. This study is the first report of K. ogawai infection in cultured olive flounder in Korea.

Paralichthys

Olive flounder

Myxosporea

Myxozoa

10.3347/kjp.2019.57.4.439

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Citations (1)

Evaluation of the effect of protease inhibitors on the viability of Miamiensis avidus using the WST-1 assay

Parasitology International (2020)

Sang Phil Shin Han Chang Sohn Chang Nam Jin Jehee Lee

Ethylenediaminetetraacetic acid

Viability assay

Olive flounder

10.1016/j.parint.2020.102093

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Citations (1)

Molecular diagnostics for verifying an etiological agent of emaciation disease in cultured olive flounder Paralichthys olivaceus in Korea

Aquaculture (2018)

Sang Phil Shin Han Chang Sohn Chang Nam Jin Bong Jo Kang Jehee Lee

Emaciation

Paralichthys

Olive flounder

Myxozoa

10.1016/j.aquaculture.2018.04.041

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Citations (14)

Sinuolinea capsularis (Myxosporea: Sinuolineidae) Isolated from Urinary Bladder of Cultured Olive Flounder Paralichthys olivaceus

Korean Journal of Parasitology (2019)

Sang Phil Shin Chang Nam Jin Han Chang Sohn Jehee Lee

Sinuolinea capsularis Davis, 1917 is myxosporean that infect the urinary system of the host fish. Insufficient morphological and molecular data of S. capsularis exits, and it is therefore difficult to make an accurate identification of the parasite. We tried a series of morphological and molecular analysis to identify an myxosporean isolated from urinary bladder of cultured olive flounder, Paralichthys olivaceus, from Jeju island in the Republic of Korea. Some of them were observed under a light microscope and SEM, and remain samples were used molecular and phylogenetic analysis. Mature spores were subspherical, measuring 13.9±0.6 μm in length and 13.8±0.8 μm in width. Two spherical polar capsules on opposite sides in the middle of the spore had a diameter range of 4.3±0.4 μm. Scanning electron microscopy revealed that spores a severely twisted the suture line. By the morphological comparison and analysis, it was identified as S. capsularis. In addition, we obtained the partial 18S rDNA of S. capsularis and first registered it in NCBI. Phylogenetic analysis showed that S. capsularis clustered with Zschokkella subclade infecting the urinary system of marine fish, and it supported the infection site tropism effect on phylogeny of marine myxosporeans as well as the origin of Sinuolinea is not monophyly.

Paralichthys

Myxosporea

10.3347/kjp.2019.57.2.127

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Citations (4)

A new species Myxodavisia jejuensis n. sp. (Myxosporea: Sinuolineidae) isolated from cultured olive flounder Paralichthys olivaceus in South Korea

Parasitology Research (2019)

Sang Phil Shin Chang Nam Jin Han Chang Sohn Hiroshi Yokoyama Jehee Lee

Paralichthys

Olive flounder

Myxosporea

10.1007/s00436-019-06454-z

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Citations (3)

Parvicapsula curvatura n. sp. in cultured olive flounder Paralichthys olivaceus and phylogenetic characteristics of the genus Parvicapsula

Diseases of Aquatic Organisms (2018)

SP Shin Chang-Nam Jin Han Chang Sohn Jehee Lee

Parvicapsula curvatura n. sp. (Myxozoa; Bivalvulida) was found in the urinary bladder of olive flounder Paralichthys olivaceus cultured in a fish farm on Jeju Island, ROK. When laterally viewed, the parasite has asymmetrical curved spores that measure 9.6-11.6 µm in length. Furthermore, it has 2 subspherical polar capsules at the apex. Based on the phenotypical traits, it is most similar to P. limandae but differs in the shape of polar capsule, locality, and host specificity (family level). BLAST analysis indicated that P. curvatura was closest to P. unicornis and P. petuniae via 18S and 28S rDNA sequences, respectively. The 18S rDNA from P. curvatura was used in molecular phylogenetic analyses of Parvicapsula spp. to examine the congruence of phylogeny with spore morphology, locality, and host specificity. The results demonstrated that the spore morphotype was correlated with the phylogeny of the genus Parvicapsula, and the parasites have speciated into an oblong and semicircular spore type.

Paralichthys

Olive flounder

Myxozoa

10.3354/dao03276

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Citations (5)

Improved multiplex PCR method for the detection of diverse Megalocytivirus in the Korea

Authorea (Authorea) (2021)

Han Chang Sohn Seongdo Lee Mun Gyeong Kwon Jee Youn Hwang Seong Don Hwang

Megalocytivirus is a genus of piscine viruses that belongs to the Iridoviridae family, and this family comprises red seabream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), and infectious spleen and kidney necrosis virus. RSIV causes high mortality and economic losses in the rock bream (Oplegnathus fasciatus) Korean aquaculture industry. The World Organization for Animal Health’s Office International des Epizooties has provided a manual for RSIV detection. However, detection against TRBIV has not been confirmed. In this study, a multiplex PCR method was established to detect two genotypes of Megalocytivirus, RSIV and TRBIV. New primer pairs were optimized for the PCR reaction. A mixture of one universal and two specific primer pairs could amplify three distinct products targeting three different genes. The sensitivity of the primer pairs was evaluated and results showed a detection limit of 2.0  105 copies for each target gene. Moreover, the primer pairs did not amplify any other viruses. The evaluation of multiplex PCR using 21 RSIV Korean isolates has shown that it can distinguish two genotypes of Megalocytivirus from 21 RSIV Korean isolates. Finally, we describe a new multiplex PCR method to detect different genotypes of Megalocytivirus simultaneously, which makes the diagnosis of viral diseases occurring in the Korean aquaculture industry more convenient.

Iridovirus

Primer (cosmetics)

10.22541/au.161248605.53028408/v1

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Citations (0)

Quantitative investigation of Enteromyxum leei (Myxozoa: Myxosporea) infection and relative condition factor in cultured olive flounder Paralichthys olivaceus (Temminck and Schlegel)

Journal of Fish Diseases (2018)

Sang Phil Shin Han Chang Sohn Chang Nam Jin Bong Jo Kang Jehee Lee

Enteromyxum leei has been reported to cause emaciation disease in various fish species. To determine the effect of parasite intensity on cultured olive flounder Paralichthys olivaceus, we investigated the relationship between the relative condition factor (rCF = CF/standard CF × 100) and parasite load with quantitative polymerase chain reaction (qPCR) and the challenge test. A total of 57 cultured olive flounders were obtained from 11 fish farms and divided into five groups based on their rCF. We investigated the parasite intensity in the posterior intestine of the fish. The parasite load was closely matched to severe loss of body weight. In addition, olive flounders were inoculated either orally or anally with intestinal scrapings of infected fish or phosphate-buffered saline. The fish were reared at natural water temperature and transferred to different tanks, and the water temperature was adjusted to 20°C after 6 weeks of inoculation. When the water temperature was increased to 20°C, the rCF decreased in the experimentally infected group. The results demonstrated that qPCR can be utilized to determine the relative abundance of E. leei in olive flounders and water temperature is an important factor to track the progress of the emaciation disease.

Paralichthys

Olive flounder

Myxozoa

Myxosporea

Emaciation

Parasite load

Trichodina

10.1111/jfd.12912

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Citations (5)