Free fatty acids (FFA), the uranyl ion, and the basic dye Rhodamine B form colored complexes, which are extractable into toluene or benzene.Fatty acids of different chain lengths above Clo and different degrees of unsaturation gave constant molar yield.Complexes in toluene alone are unstable, especially in the light, but a small amount of aqueous uranyl acetate stabilizes them sufficiently for determination.At constant uranyl and Rhodamine B concentrations, a plot of optical density vs. FFA concentration yields two straight lines of different slope, Le., a biphasic standard curve.Phospholipids interfere, and must be removed with zeolite during FFA extraction.Recovery of FFA added to rat plasma was very similar to that with titration.Assay of rat and dog plasma samples under fasting and fed conditions gave good agreement with the titration method.Values of human plasma samples tended to be higher by the colorimetric procedure; a few samples gave significant disagreement.The method compares well with previous methods in sensitivity and accuracy, and offers advantages in speed, simplicity, and possibly specificity.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHypocholesterolemic indole-2-carboxylic acidsTakashi Kariya, J. Martin Grisar, Norbert L. Wiech, and Thomas R. BlohmCite this: J. Med. Chem. 1972, 15, 6, 659–662Publication Date (Print):June 1, 1972Publication History Published online1 May 2002Published inissue 1 June 1972https://pubs.acs.org/doi/10.1021/jm00276a024https://doi.org/10.1021/jm00276a024research-articleACS PublicationsRequest reuse permissionsArticle Views242Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Abstract Die Synthese der im Titel genannten Verbindung (Va) und des entsprechenden Thions (Vb) gelingt, ausgehend von (Ia), wie im Formelschema angegeben.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNaphthylalkyl lactamimides as inhibitors of blood platelet aggregationEdward M. Roberts, J. Martin Grisar, Robert D. Mackenzie, George P. Claxton, and Thomas R. BlohmCite this: J. Med. Chem. 1972, 15, 12, 1270–1273Publication Date (Print):December 1, 1972Publication History Published online1 May 2002Published inissue 1 December 1972https://pubs.acs.org/doi/10.1021/jm00282a017https://doi.org/10.1021/jm00282a017research-articleACS PublicationsRequest reuse permissionsArticle Views121Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Stypven, Russell’s viper venom, when added to plasma activates factor X and factor V. The rate of thrombin formation and clotting time are dependent on the amount of activity of platelet factor 3 (PF-3), a cofactor for the prothrombin-converting enzyme complex. The modified Stypven test reported not only sensitizes the procedure by plasma dilution, but also quantitates the amount of PF-3 activity present as it relates to the maximum amount of PF-3 made available by sonication of platelet-rich plasma, or to a standard PF-3 preparation.
