Ana M. Coimbra

University of Trás-os-Montes and Alto Douro

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Luís Félix

University of Trás-os-Montes and Alto Douro

Sandra M. Monteiro

University of Trás-os-Montes and Alto Douro

L. Antunes

University of Trás-os-Montes and Alto Douro

Ana Luzio

University of Trás-os-Montes and Alto Douro

A. Fontaínhas‐Fernandes

University of Trás-os-Montes and Alto Douro

Manuela Matos

University of Trás-os-Montes and Alto Douro

Ana M. Valentim

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

Teresa Neuparth

Universidade do Porto

Marlene Pinheiro

Universidade do Porto

Dércia Santos

University of Trás-os-Montes and Alto Douro

Cooperative Institutions

University of Trás-os-Montes and Alto Douro

Universidade do Porto

University of Coimbra

Centro Interdisciplinar de Investigação Marinha e Ambiental

United States Military Academy

Association for the Development of Douro Viticulture

KU Leuven

Merck & Co., Inc., Rahway, NJ, USA (United States)

Yale University

Rede de Química e Tecnologia

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Biochemical blood parameters: are they effective biomarkers of chronic toxicity?

Conceição Fernandes A. Fontaínhas‐Fernandes Ana M. Coimbra María José Saavedra Maria Antónia Salgado

Chronic toxicity

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Chronic exposure to environmentally relevant levels of simvastatin disrupts zebrafish brain gene signaling involved in energy metabolism

Journal of Toxicology and Environmental Health (2020)

Susana Barros Ana M. Coimbra Nélson Alves Marlene Pinheiro José Benito Quintana

Simvastatin (SIM), a hypocholesterolaemic drug belonging to the statins group, is a widely prescribed pharmaceutical for prevention of cardiovascular diseases. Several studies showed that lipophilic statins, as SIM, cross the blood-brain barrier and interfere with the energy metabolism of the central nervous system in humans and mammalian models. In fish and other aquatic organisms, the effects of SIM on the brain energy metabolism are unknown, particularly following exposure to low environmentally relevant concentrations. Therefore, the present study aimed at investigating the influence of SIM on gene signaling pathways involved in brain energy metabolism of adult zebrafish (Danio rerio) following chronic exposure (90 days) to environmentally relevant SIM concentrations ranging from 8 ng/L to 1000 ng/L. Real-time PCR was used to determine the transcript levels of several genes involved in different pathways of the brain energy metabolism (glut1b, gapdh, acadm, accα, fasn, idh3a, cox4i1, and cox5aa). The findings here reported integrated well with ecological and biochemical responses obtained in a parallel study. Data demonstrated that SIM modulates transcription of key genes involved in the mitochondrial electron transport chain, in glucose transport and metabolism, in fatty acid synthesis and β-oxidation. Further, SIM exposure led to a sex-dependent transcription profile for some of the studied genes. Overall, the present study demonstrated, for the first time, that SIM modulates gene regulation of key pathways involved in the energy metabolism in fish brain at environmentally relevant concentrations.

Danio

10.1080/15287394.2020.1733722

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Citations (19)

Circulating thyroid hormone levels and iodothyronine deiodinase activities in Nile tilapia (Oreochromis niloticus) following dietary exposure to Endosulfan and Aroclor 1254

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology (2005)

Ana M. Coimbra M.A. Reis-Henriques Veerle Darras

Nile tilapia

Endosulfan

Iodothyronine deiodinase

10.1016/j.cca.2005.04.006

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Citations (66)

Toxicology Letters (2017)

Luís Félix Cindy Serafim Ana M. Valentim L. Antunes Manuela Matos

10.1016/j.toxlet.2017.07.888

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Refinement Techniques in Zebrafish Anaesthesia – Results from a Pilot Study

Microscopy and Microanalysis (2015)

Carla Santos Luís Félix Marco A. C. Neves Ana M. Coimbra Ana M. Valentim

Regional anaesthesia

10.1017/s1431927615014270

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Review on the use of zebrafish embryos to study the effects of anesthetics during early development

Critical Reviews in Toxicology (2019)

Luís Félix Ana M. Coimbra Ana M. Valentim L. Antunes

Over the years, the potential toxicity of anesthetics has raised serious concerns about its safe use during pregnancy. As evidence emerged from research in animal models, showing that some anesthetic drugs are potential teratogenic, the determination of the risk of exposures to anesthetic drugs at early life stages became mandatory. However, due to inaccessibility and ethical constrains related to experimental conditions, the use of early life stages in mammalian models is limited. In this regard, some animal and nonanimal models have been suggested to surpass mammalian use in experimentation. Among them, the zebrafish embryo test has been recognized as a promising alternative in toxicology research, as well as an inexpensive and practical test. Substantial information collected from developmental research following compounds exposure, has contributed to the application of zebrafish assays in research, although only a few studies have focused on the use of early life stages of zebrafish to evaluate the developmental effects of anesthetics. Based on the recent advances of science and technology, there is a clear potential for zebrafish early life stages to provide new insights into anesthetics teratogenicity. This review provides an overview of recent anesthesia research using zebrafish embryos, demonstrating its usefulness to the anesthesia field, discussing the recent findings on various aspects related to the effects of anesthetics during early life development and the strengths and limitations of this model system.

Animal testing

Animal model

10.1080/10408444.2019.1617236

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Citations (19)

Effects of short-term exposure to genistein and overfeeding diet on the neural and retinal progenitor competence of adult zebrafish (Danio rerio)

Neurotoxicology and Teratology (2021)

Ana Luzio Mariana Sarto Figueiredo Manuela Matos Ana M. Coimbra Ana Rita Álvaro

Danio

Progenitor

10.1016/j.ntt.2021.107030

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Citations (8)

Mycobacterium szulgai: A Rare Cause of Non-Tuberculous Mycobacteria Disseminated Infection

Journal of Medical Cases (2022)

Ana Luísa Nunes Ana M. Coimbra Rúben Carvalho Carolina Figueiredo Vânia Almeida

Mycobacterium szulgai (MS) is a rare and slow-growing type of non-tuberculous mycobacteria (NTM), with a human isolation prevalence of less than 0.2% of all NTM cases. MS may cause pulmonary infection, extra-pulmonary localized disease involving the skin, lymph nodes, bone, synovial tissue or kidneys and disseminated infection, when two or more organs are affected. When disseminated infection is present, the patients usually have an underlying immunosuppressive condition. The authors report the case of a 25-year-old patient with systemic lupus erythematosus, presenting with recurrent fever, non-productive coughing, weight loss and asthenia, as well as two violaceous plaques with superficial ulceration in the gluteal region. MS was isolated from the bronchial lavage and skin biopsy cultures, confirming the rare disseminated form of MS infection. After 10 months of follow-up on isoniazid, rifampin, ethambutol and pyrazinamide, no signs of relapse were evident. To date, only 16 other cases of MS disseminated disease have been reported.

Mycobacterium avium complex

10.14740/jmc3885

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Citations (5)

Zum 85. Geburtstag von Prof. Dr. J. Meller

Ophthalmologica (1959)

A. Neetens G. Velický J. Sebestyén Ettore De Berardinis Giulio Bonavolontà

10.1159/000303653

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A new osteophyte segmentation method with applications to an anterior cruciate ligament transection rabbit femur model via micro-CT imaging

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2010)

Guoyuan Liang Jacob M. Elkins Ana M. Coimbra Le T. Duong Donald S. Williams

Osteophyte is an additional bony growth on a normal bone surface limiting or stopping motion in a deteriorating joint. Detection and quantification of osteophytes from CT images is helpful in assessing disease status as well as treatment and surgery planning. However, it is difficult to segment osteophytes from healthy bones using simple thresholding or edge/texture features in CT imaging. Here, we present a new method, based on active shape model (ASM), to solve this problem and evaluate its application to ex vivo μCT images in an ACLT rabbit femur model. The common idea behind most ASM based segmentation methods is to first build a parametric shape model from a training dataset and during application, find a shape instance from the model that optimally fits to target image. However, it poses a fundamental difficulty for the current application because a diseased bone shape is significantly altered at regions with osteophyte deposition misguiding an ASM method that eventually leads to suboptimum segmentation results. Here, we introduce a new partial ASM method that uses bone shape over healthy regions and extrapolate its shape over diseased region following the underlying shape model. Once the healthy bone region is detected, osteophyte is segmented by subtracting partial-ASM derived shape from the overall diseased shape. Also, a new semi-automatic method is presented in this paper for efficiently building a 3D shape model for rabbit femur. The method has been applied to μCT images of 2-, 4-, and 8-week post ACLT and sham-treated rabbit femurs and results of reproducibility and sensitivity analyses of the new osteophyte segmentation method are presented.

Rabbit (cipher)

10.1117/12.844440

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