Matt Trau

University of Queensland

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Alain Wuethrich

University of Queensland

Muhammad J. A. Shiddiky

Charles Sturt University

Darren Korbie

University of Queensland

Abu Ali Ibn Sina

University of Queensland

Kevin M. Koo

University of Queensland

Yuling Wang

Xinyang Normal University

Bronwyn J. Battersby

University of Queensland

Eugene J. H. Wee

University of Queensland

Laura G. Carrascosa

University of Queensland

Lisbeth Grøndahl

University of Queensland

Cooperative Institutions

University of Queensland

289

Queensland University of Technology

University of Melbourne

Brisbane School of Theology

QIMR Berghofer Medical Research Institute

Translational Research Institute

Institute of Bioengineering and Nanotechnology

Monash University

Royal Brisbane and Women's Hospital

Princess Alexandra Hospital

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Fluorescent organosilica micro- and nanoparticles with controllable size

Journal of Colloid and Interface Science (2007)

Robert Vogel Peter P. T. Surawski Brad Littleton Chris R. Miller Gwendolyn Lawrie

10.1016/j.jcis.2007.01.092

Cite

Citations (55)

Characterising the phenotypic evolution of circulating tumour cells during treatment

Nature Communications (2018)

Simon Chang‐Hao Tsao Jing Wang Yuling Wang Andreas Behren Jonathan Cebon

Real-time monitoring of cancer cells' phenotypic evolution during therapy can provide vital tumour biology information for treatment management. Circulating tumour cell (CTC) analysis has emerged as a useful monitoring tool, but its routine usage is restricted by either limited multiplexing capability or sensitivity. Here, we demonstrate the use of antibody-conjugated and Raman reporter-coated gold nanoparticles for simultaneous labelling and monitoring of multiple CTC surface markers (named as "cell signature"), without the need for isolating individual CTCs. We observe cell heterogeneity and phenotypic changes of melanoma cell lines during molecular targeted treatment. Furthermore, we follow the CTC signature changes of 10 stage-IV melanoma patients receiving immunological or molecular targeted therapies. Our technique maps the phenotypic evolution of patient CTCs sensitively and rapidly, and shows drug-resistant clones having different CTC signatures of potential clinical value. We believe our proposed method is of general interest in the CTC relevant research and translation fields.

Circulating tumor cell

Targeted Therapy

10.1038/s41467-018-03725-8

Cite

Citations (100)

A Microfluidic SERS Assay to Characterize the Phenotypic Heterogeneity in Cancer-Derived Small Extracellular Vesicles

Methods in molecular biology (2023)

Jing Wang Matt Trau Alain Wuethrich

Extracellular Vesicles

Nanoparticle tracking analysis

Nanobiotechnology

10.1007/978-1-0716-3271-0_17

Cite

Citations (2)

Analysis of secreted small extracellular vesicles from activated human microglial cell lines reveals distinct pro‐ and anti‐inflammatory proteomic profiles

PROTEOMICS (2024)

Xueming Niu Zhen Zhang Quan Zhou Alain Wuethrich Richard J. Lobb

Abstract Microglia are a specialized population of innate immune cells located in the central nervous system. In response to physiological and pathological changes in their microenvironment, microglia can polarize into pro‐inflammatory or anti‐inflammatory phenotypes. A dysregulation in the pro‐/anti‐inflammatory balance is associated with many pathophysiological changes in the brain and nervous system. Therefore, the balance between microglia pro‐/anti‐inflammatory polarization can be a potential biomarker for the various brain pathologies. A non‐invasive method of detecting microglia polarization in patients would have promising clinical applications. Here, we perform proteomic analysis of small extracellular vesicles (sEVs) derived from microglia cells to identify sEVs biomarkers indicative of pro‐inflammatory and anti‐inflammatory phenotypic changes. sEVs were isolated from microglia cell lines under different inflammatory conditions and analyzed by proteomics by liquid chromatography with mass spectrometry. Our findings provide the potential roles of sEVs that could be related to the pathogenesis of various brain diseases.

Extracellular Vesicles

10.1002/pmic.202300094

Cite

Citations (1)

Microscopic patterning of orientated mesoscopic silica through guided growth

Nature (1997)

Matt Trau Nan Yao E. Kim Yingdong Xia George M. Whitesides

Mesoscopic physics

10.1038/37764

Cite

Citations (375)

Rapid DNA detection by beacon-assisted detection amplification

Nature Protocols (2011)

Ashley R. Connolly Matt Trau

Multiple displacement amplification

Molecular beacon

Primer (cosmetics)

DNA clamp

Sequencing by ligation

Primer dimer

A-DNA

10.1038/nprot.2011.326

Cite

Citations (35)

Quantitative data analysis methods for bead-based DNA hybridization assays using generic flow cytometry platforms

Journal of Molecular Diagnostics (2007)

Simon R. Corrie Gwendolyn Lawrie Bronwyn J. Battersby K A Ford Andreas Rühmann

Quantitative Analysis

Bead

Source

Cite

Citations (0)

A material odyssey for 3D nano/microstructures: two photon polymerization based nanolithography in bioapplications

Applied Materials Today (2020)

Caizhi Liao Alain Wuethrich Matt Trau

Biomolecule

10.1016/j.apmt.2020.100635

Cite

Citations (82)

SERS Multiplex Profiling of Melanoma Circulating Tumor Cells for Predicting the Response to Immune Checkpoint Blockade Therapy

Analytical Chemistry (2022)

Junrong Li Alain Wuethrich Zhen Zhang Jing Wang Lynlee L. Lin

Immune checkpoint blockade (ICB) therapy has achieved remarkable success in many cancers including melanoma. However, ICB therapy benefits only a small proportion of patients and produces severe side effects for some patients. Thus, there is an urgent need to identify patients who are more likely to respond to ICB therapy to improve outcomes and minimize side effects. To predict ICB therapy responses, we design a surface-enhanced Raman scattering (SERS) assay for multiplex profiling of circulating tumor cells (CTCs) under basal and interferon-γ (IFN-γ) stimulation. Through simultaneous ensemble and single-cell measurements of CTCs, the SERS assay can reveal tumor heterogeneity and offer a comprehensive CTC phenotype for decision-making. Anisotropic gold-silver alloy nanoboxes are utilized as SERS plasmonic substrates for improved signal readouts of CTC surface biomarkers. By generating a unique CTC signature with four surface biomarkers, the developed assay enables the differentiation of CTCs from three different patient-derived melanoma cell lines. Significantly, in a cohort of 14 melanoma patients who received programmed cell death-1 blockade therapy, the changes of CTC signature induced by IFN-γ stimulation to CTCs show the potential to predict responders. We expect that the SERS assay can help select patients for receiving ICB therapy in other cancers.

Multiplex

Immune checkpoint

Circulating tumor cell

Targeted Therapy

10.1021/acs.analchem.2c02398

Cite

Citations (26)

Porous functionalised silica particles: a potential platform for biomolecular screening

Chemical Communications (2005)

Angus P. R. Johnston Bronwyn J. Battersby Gwendolyn Lawrie Matt Trau

Novel, porous, functionalised silica particles have been developed with controlled morphology, which promote covalent attachment of fluorescent dyes which can act as an optical barcode.

Barcode

10.1039/b415376f

Cite

Citations (100)