Abstract A novel fluorescent probe, LCH , based on dicyanisophorone and carbazole, was prepared for the visual detection of Cu 2+ . The probe LCH could recognize Cu 2+ by fluorescence quenching in EtOH/H 2 O (1/4, v/v) solution, which could be easily identified under the 365 nm UV lamp, and the detection limit was as low as 0.785 μM. The recognition mechanism of probe LCH with Cu 2+ was determined by combining 1 H NMR titration, MS, and theoretical calculations. Practical application experiments showed that probe LCH could be used to detect Cu 2+ in the test strip experiments. Cell imaging experiments showed that the probe LCH owned good cell permeability and could be applied to the imaging of Cu 2+ in HepG2 cells. In addition, fluorescence colocalization experiments showed that LCH could target lipid droplets. These results indicate that the probe LCH will have a good application prospect in environmental detection and clinical medicine.
Abstract Integrating perovskite solar cells with crystalline silicon bottom cells in a monolithic two‐terminal tandem configuration enables power conversion efficiency (PCE) surpassing the theoretical limits of single‐junction cells. However, wide bandgap (WBG) perovskite films face challenges related to phase stability and open circuit voltage ( V OC ) deficit, particularly due to severe non‐radiative recombination at the perovskite/C 60 interface. Here, the interfacial defects are passivated by incorporating a reactive passivator that reacts with lead halides to form low‐dimensional phases. The target product obtained by optimizing the reaction temperature not only suppresses recombination across the interface, but also facilitates the transfer of charge carriers. More importantly, this product can suppress phase segregation of WBG perovskite films under exposure to light illumination and moisture. This strategy enables a high V OC of 1.25 V for WBG perovskite device based on polymer hole transport layer and a certified stabilized PCE of 30.52% for a monolithic perovskite/silicon tandem solar cell. The unencapsulated tandem device retains 94% of its initial PCE over 200 h under continuous 1‐sun full spectrum illumination in air, demonstrating the improved phase stability.
Disruption of the physical barrier of intestinal epithelial cells is a hallmark of ulcerative colitis (UC). Deubiquitinating enzymes (DUBs), which modify the stability, localization, or activity of substrates by removing ubiquitin chains, have been involved in the development of UC. However, the biological role of DUBs in intestinal epithelial cells (IECs) has yet to be extensively investigated. In this work, we revealed that USP2 as the most differentially expressed DUB in the colon of mice with dextran sodium sulfate (DSS)-induced colitis and was downregulated mainly in the intestinal epithelium. Notably, the expression of USP2 was correlated with the levels of tight junction (TJ) proteins. Furthermore we showed that USP2 expression was reduced in the intestine of mice with acute colitis induced by DSS, as well as in IECs stimulated with LPS. Overexpression of USP2 restored the LPS-induced the reduction of TJPs. Mechanistically, USP2 attenuates the activation of NF-κB and the phosphorylation of the myosin light chain, possibly by reducing the K63-linked polyubiquitin chain on the tumour necrosis factor receptor-associated factor 6 (TRAF6). In vivo, colonic-specific overexpression of USP2 in mice ameliorated DSS-induced barrier damage. Together, our study proposed USP2 as a new deubiquitinating enzyme molecule involved in the course of UC regulating the function of IECs during the progression of UC.
Photothermal therapy (PTT) combined with chemotherapy has been highly desirable to improve the tumor treatment efficacy. Here, we report a novel controlled release nano-carrier system named Se-PEG-Au NPs-DOX (SePAD) for achieving chemo-photothermal synergistic tumor therapy. In this system, the diselenide-containing co-polymers (mPEG-Se–Se-PEGm) were anchored onto the surface of Au NPs via AuSe interactions and the soluble chemotherapeutic doxorubicin (Dox) was loaded into the monolayers. This structure is proved to be stable in a high biological thiols environment. Strikingly, the SePAD presents an ideal efficient photothermal capability and a controllable Dox release behavior by dynamic AuSe interaction in GSH-rich tumor cells when irradiated under 808 nm NIR laser. Following, the results of in vitro and in vivo experiments all demonstrat the superior antitumor properties of SePAD in murine breast cancer. Thus, this system provides a promising strategy to realize chemo-photothermal synergistic combination therapy for breast tumors.
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