Huiwan Han

Institute of Chemistry

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Guoquan Liu

Shanghai Jiao Tong University

Guoquan Liu

ARC Centre of Excellence for Plant Success in Nature and Agriculture

Dihua Shangguan

Chinese Academy of Sciences

Peter G. Osborne

Kaohsiung Medical University

Ru‐Song Zhao

Qilu University of Technology

Yu Xiao

Peking Union Medical College Hospital

Yuhui Dong

Chinese Academy of Sciences

Shaoxiang Xiong

Chinese Academy of Sciences

Rui Zhao

John H. Stroger, Jr. Hospital of Cook County

Bing Zang

Center for Disease Control

Cooperative Institutions

Chinese Academy of Sciences

Institute of Chemistry

Peking University

Beijing National Laboratory for Molecular Sciences

University of Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

University of the Andes

Sun Yat-sen University

Shanghai Jiao Tong University

Sichuan University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides

Journal of Chromatography A (2001)

Dihua Shangguan Huiwan Han Ru‐Song Zhao Yingxin Zhao Shaoxiang Xiong

Hydrazone

Hydrazine (antidepressant)

Gradient elution

10.1016/s0021-9673(00)01208-5

Cite

Citations (62)

Hyperoxia caused by microdialysis perfusion decreased striatal monoamines: involvement of oxidative stress

Neurochemistry International (2003)

Lei Wang Peter G. Osborne Yu Xiao Dihua Shangguan Rui Zhao

Microdialysis

Hyperoxia

Oxygen tension

10.1016/s0197-0186(02)00159-6

Cite

Citations (4)

Determination of carbohydrates as their 3-aminophthalhydrazide derivatives by capillary zone electrophoresis with on-line chemiluminescence detection

Journal of Chromatography A (2003)

Xiaoyan Wang Qiang Wang Yi Chen Huiwan Han

Luminol

Sodium hydroxide

Monosaccharide

Peroxyoxalate

10.1016/s0021-9673(03)00273-5

Cite

Citations (28)

Analysis of glucose and lactate in hippocampal dialysates of rats during the operant conditioned reflex using microdialysis

Neurochemistry International (2003)

Yuhui Dong Lei Wang Dihua Shangguan Yu Xiao Rui Zhao

Microdialysis

10.1016/s0197-0186(02)00192-4

Cite

Citations (16)

Detection of trace Cuii by a designed calix[4]arene based fluorescent reagent

New Journal of Chemistry (2002)

Huimin Ma Quanli Ma Meihong Su Lihua Nie Huiwan Han

A highly Cu2+ selective calix[4]arene based fluorescent reagent, 5,17-bis(4-methylcoumarin-7-azo)-25,26,27,28-tetrahydroxycalix[4]arene, has been designed, synthesized and evaluated. The reagent exhibits excellent selectivity for Cu2+ over a wide range of alkali, alkaline earth and other transition metal ions. Quenching of its fluorescence due to a strong Cu2+ affinity, induced binding and selective redox reaction is not influenced by the presence of 20- to 10000-fold excesses of Al3+, Ca2+, Cd2+, Co2+, Cr3+, Hg2+, K+, Mg2+, Mn2+, NH4+, Ni2+, Pb2+, Zn2+, Cl−, NO3−, CO32−, SO42− or PO43−. Furthermore, with this fluorescent reagent a simple, sensitive and highly selective method has been developed for measuring trace Cu2+ in real biological fluids. The combination of multiple selective responses presented here may provide a useful design strategy for preparing selective reagents of other species.

Calixarene

Alkaline earth metal

10.1039/b203331c

Cite

Citations (15)

Derivatization and Fluorescence Detection of Amino Acids and Peptides with 9-Fluorenylmethyl Chloroformate on the Surface of a Solid Adsorbent

Analytical Chemistry (2001)

Dihua Shangguan Yingxin Zhao Huiwan Han Ru‐Song Zhao Guoquan Liu

An approach that exploits the surface of a solid adsorbent is proposed for precolumn FMOC derivatization of amino acids and peptides. Amino acids (Ser, Glu, GABA, Val, Phe, Lys) and two neuropeptides (substance P and Leuenkephalin) were adsorbed on alkaline silica gel cartridges. After drying, they were reacted with 9-fluorenyl-methyl chloroformate (FMOC-Cl) in toluene. After washing off the excess FMOC-Cl with ethyl acetate, the derivatives were eluted with aqueous eluant. The eluates were separated and detected by means of HPLC with fluorescence detection. Compared with the traditional derivatization in the liquid phase, the extent of formation of byproducts of FMOC-Cl with water was greatly decreased, and the excess FMOC-Cl was eliminated completely.

Chloroformate

10.1021/ac001243c

Cite

Citations (40)

Effects of salicylate on serotoninergic activities in rat inferior colliculus and auditory cortex

Hearing Research (2003)

Junxiu Liu Xuepei Li Lei Wang Yuhui Dong Huiwan Han

Inferior Colliculi

10.1016/s0378-5955(02)00708-6

Cite

Citations (43)

Affinity capillary electrophoresis coupled with on-line microdialysis by attachable electrode

Electrophoresis (1999)

Mei Fang Sheng Li Huiwan Han Yu Xiao Rui Zhao

Microdialysis

Line (geometry)

10.1002/(sici)1522-2683(19990701)20:9<1846::aid-elps1846>3.3.co;2-g

Cite

Citations (0)

Oxygen-sensing microdialysis probe for in vivo use

Journal of Neuroscience Research (2001)

Peter G. Osborne Xiaofeng Li Yuanzong Li Huiwan Han

Electrochemical conditions were optimized to allow the metal tube used for the shaft of commercial microdialysis (MD) probes to be coated with gold. In in vitro tests with phosphate-buffered Ringer's solution using double differential pulse amperometry (DDPA), the gold-coated shafts were capable of specifically measuring the reduction of oxygen and the oxidation of ascorbic acid in the presence of high concentrations of potentially interfering endogenous substances. By using fixed-potential amperometry (FPA), the gold-plated shaft also measured oxygen with minimal interference from high concentrations of potentially interfering endogenous substances. Concentric design MD probes were constructed that used a metal shaft (O.D = 0.4 mm), fused silica inlet and outlet tubes, and a 1.5 mm dialyzing membrane (O.D = 0.2 mm). A 0.5–0.7 mm gold collar was electroplated onto the metal shaft approximately 0.5 mm above the dialyzing membrane. The nongold outer surface of the MD probe was coated with an insulating polymer. In vivo tests demonstrated that DDPA was not suitable for use with this gold microdialyzing electrode (GMDE). However, brain oxygen levels were satisfactorily measured using FPA. In urethane-anesthetized rats, the reduction current to oxygen in the striatum was increased by brief (1 min) inhalation of O2 or CO2 and decreased by inhalation of N2. Transient application of noxious stimuli (foot pinch) increased cerebral O2, whereas bilateral carotid artery occlusion and death decreased striatal O2. The responses of the GMDE were indistinguishable from the reduction current simultaneously measured from a conventional carbon fiber electrode implanted adjacent to the gold-plated area of the MD shaft. Basal levels of striatal O2 were 20 ± 5 μM (n = 4) for the GMDE and 30 ± 11 μM (n = 3) for the carbon fiber. The GMDE was robust and could be used for at least three animals. This technique can be used to provide information about the oxygen status of the tissue adjacent to the dialyzing membrane without the need for implantation of an additional electrode. J. Neurosci. Res. 63:224–232, 2001. © 2001 Wiley-Liss, Inc.

Amperometry

Microdialysis

10.1002/1097-4547(20010115)63:2<224::aid-jnr1015>3.0.co;2-7

Cite

Citations (12)

A NOVEL METHOD FOR PREPARATION AND CHARACTERIZATION OF RESTRICTED-ACCESS MEDIA-ALKYL-DIOL SILICA (ADS)

Journal of Liquid Chromatography &amp Related Technologies (2001)

Xin Yu Rui Zhao Huiwan Han Guoquan Liu

A novel method had been developed to prepare ADS packings. Different alkyl-chains were bound onto Develosil60-5μ silica (particle size dp = 5 μm, s = 400–450m2/g) with γ-glycidoxypropy-ltrimethoxysilane as coupling agent and hydrophilic diol phase precursor. Characterization of the packings was carried out with elemental analysis, X-Ray Photoelectron Spectroscopy (XPS), Fourier Transform Infrared (FT-IR), solid-state 13C, and 29Si CP-MAS NMR. Chromatographic tests confirm the essence of the packings and RAS behavior.

Characterization

10.1081/jlc-100104902

Cite

Citations (2)