Marcin Michalak

University of Warmia and Mazury in Olsztyn

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Paweł Chmielarz

Institute of Dendrology

Beata P. Plitta‐Michalak

University of Warmia and Mazury in Olsztyn

Jan Barciszewski

Institute of Bioorganic Chemistry, Polish Academy of Sciences

Beata P. Plitta

Institute of Dendrology

Mirosława Z. Naskręt-Barciszewska

Institute of Bioorganic Chemistry, Polish Academy of Sciences

Szymon Kotlarski

Institute of Dendrology

T. Tylkowski

Polish Academy of Sciences

K. Bojarczuk

Polish Academy of Sciences

Jan Suszka

Institute of Dendrology

Teresa Hazubska-Przybył

Institute of Dendrology

Cooperative Institutions

Polish Academy of Sciences

Institute of Dendrology

Universidade Federal do Espírito Santo

Polish Academy of Learning

University of Bern

University of Milan

Sichuan University

East China University of Technology

Institute of Organic Chemistry

Beijing University of Chemical Technology

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DNA methylation of Quercus robur L. plumules following cryo-pretreatment and cryopreservation

Plant Cell Tissue and Organ Culture (PCTOC) (2013)

Beata P. Plitta Marcin Michalak Mirosława Z. Naskręt-Barciszewska Jan Barciszewski Paweł Chmielarz

Quercus robur

Germ plasm

10.1007/s11240-013-0417-9

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Citations (20)

Wpływ systemu uprawy na zawartość i proporcje składników pokarmowych w ziarnie jęczmienia jarego

Journal of Elementology (2003)

E. Makarska Marcin Michalak

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Epigenetic Marks, DNA Damage Markers, or Both? The Impact of Desiccation and Accelerated Aging on Nucleobase Modifications in Plant Genomic DNA

Cells (2022)

Beata P. Plitta‐Michalak Monika Litkowiec Marcin Michalak

Modifications of DNA nucleobases are present in all forms of life. The purpose of these modifications in eukaryotic cells, however, is not always clear. Although the role of 5-methylcytosine (m5C) in epigenetic regulation and the maintenance of stability in plant genomes is becoming better understood, knowledge pertaining to the origin and function of oxidized nucleobases is still scarce. The formation of 5-hydroxymetylcytosine (hm5C) in plant genomes is especially debatable. DNA modifications, functioning as regulatory factors or serving as DNA injury markers, may have an effect on DNA structure and the interaction of genomic DNA with proteins. Thus, these modifications can influence plant development and adaptation to environmental stress. Here, for the first time, the changes in DNA global levels of m5C, hm5C, and 8-oxo-7,8-dihydroguanine (8-oxoG) measured by ELISA have been documented in recalcitrant embryonic axes subjected to desiccation and accelerated aging. We demonstrated that tissue desiccation induces a similar trend in changes in the global level of hm5C and 8-oxoG, which may suggest that they both originate from the activity of reactive oxygen species (ROS). Our study supports the premise that m5C can serve as a marker of plant tissue viability whereas oxidized nucleobases, although indicating a cellular redox state, cannot.

Nucleobase

genomic DNA

10.3390/cells11111748

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Successful In Vitro Shoot Multiplication of Quercus robur L. Trees Aged up to 800 Years

Plants (2023)

Paweł Chmielarz Szymon Kotlarski Ewa Marzena Kalemba João Paulo Rodrigues Martins Marcin Michalak

The conservation of the genetic resources of old trees is crucial to their ecological role but is extremely difficult, especially for oak species (Quercus spp.) displaying recalcitrance in seed and vegetative propagation methods. Our study aimed to assess the regenerative potential of Quercus robur trees of different ages (up to 800 years) during micropropagation. We also aimed to determine how in vitro conditions can influence in vitro regeneration responses. Lignified branches collected from 67 selected trees were cultivated ex vitro in culture pots at 25 °C to obtain epicormic shoots (explant sources). The explants were cultivated on an agar medium supplemented with 0.8 mg L-1 6-benzylaminopurine (BAP) for at least 21 months. In a second experiment, two different shoot multiplication conditions (temporary immersion-RITA® bioreactor and agar medium) and two culture medium formulations (Woody Plant Medium and modified Quoirin and Lepoivre medium) were tested. The results showed that the mean length of the epicormic shoots obtained in a pot culture was a function of donor age and was similar among the group of younger trees (ca. 20-200 years), and varied between older trees (ca. 300-800 years). The efficiency of in vitro shoot multiplication strictly depended on the genotype. A sustainable in vitro culture (defined as survival after 6 months) was only possible for half of the tested old donor trees, even when they survived the first month of in vitro growth. A continuous monthly increase in the number of in vitro cultured shoots was reported in younger oaks and in some old oaks. We found a significant effect of the culture system and the macro- and micronutrient composition on in vitro shoot growth. This is the first report demonstrating that the in vitro culture can be successfully applied to the propagation of even 800-year-old pedunculate oak trees.

Quercus robur

10.3390/plants12122230

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Linked PDF of Table of Contents

Synthesis (2022)

Andryj M. Borys Eva Hevia Regina Westphal Eva Venturini Filho Medici

Mechanisms of the Nickel-Catalysed Hydrogenolysis and Cross-Coupling of Aryl Ethers Ni OMe Nu Nucleophile L n Ni(II) Ar O Me LA L n Ni(0) Ar OMe Nu Ni(I) L n Nu Oxidative addition Lewis acid assisted Anionic nickelates Ni(I) intermediates

Table (database)

10.1055/s-0041-1737608

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SOMATIC EMBRYOGENESIS AND CRYOPRESERVATION OF ORNAMENTAL PICEA SPECIES: MODERN METHODS OF PROPAGATION AND LONG-TERM STORAGE

Acta Horticulturae (2012)

Teresa Hazubska-Przybył K. Bojarczuk Paweł Chmielarz Marcin Michalak

ISHS XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Advances in Ornamentals, Landscape and Urban Horticulture SOMATIC EMBRYOGENESIS AND CRYOPRESERVATION OF ORNAMENTAL PICEA SPECIES: MODERN METHODS OF PROPAGATION AND LONG-TERM STORAGE

Ornamental plant

10.17660/actahortic.2012.937.88

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Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Annals of Forest Science (2015)

Joanna Mucha Agata Szymańska Marcin Zadworny T. Tylkowski Marcin Michalak

Abstract Key message Seed storage temperature influences root anatomy of the endangered Populus nigra , and consequently may alter nutrient absorption. A lower temperature during seed storage (−20 and −196 °C) may preserve the potential for a suitable root system development after germination. Context Seed storage conditions can be an important determinant of later seedling growth of Populus nigra L., an endangered tree species. Aims We tested whether long-term seed storage temperature, −10, −20 or −196 °C, affects the pattern of seedling root traits responsible for resource acquisition as compared to seedlings of fresh seeds. Methods We analysed the morphology, anatomy, degree of mycorrhizal colonization, and biochemical composition of roots developed from seed stored for 24 months at five different temperatures (from 3 to −196 °C) commonly used to preserve genetic resources. Results Except for root anatomy, we found no relationship between seed storage temperature and the root traits of seedlings. Among the various storage conditions, the proportion of roots with primary development in the first four orders was similar in seedlings developed from fresh seeds of from seeds stored at −196 or −20 °C. Nitrogen content in the roots was positively correlated with the proportion of (i) roots with primary development and (ii) the cortex width in the root diameter. Conclusions Higher temperatures during seed storage reduced the proportion of roots with absorptive function (with primary development). Therefore, for preservation of P. nigra seeds we recommend lower temperatures such as −20 and −196 °C.

10.1007/s13595-015-0470-0

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Citations (9)

Kriogeniczne przechowywanie nasion

Sylwan (2013)

Beata P. Plitta Marcin Michalak Szymon Kotlarski Paweł Chmielarz

Nasion

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Effect of different conditions of storage on seed viability and seedling growth of six wild fruit woody plants. Figshare dataset

Figshare (2019)

Mikołaj Krzysztof Wawrzyniak Marcin Michalak Paweł Chmielarz

There are two datasets in this repository:
1. germination_stored_seeds - it is germination data of six wild fruit species after storage (Nonstored, 0.5 y, 1 y, 2 y and 3 y) at different seed moistire content (approximately 5%, 8% and 11%) and in different storage temperature (-3°, -18° and -196°C). We recorded germination (root apperance), seedling emergence (both root and shoot apperance). Seedling emergence was being recorded both at laboratory and nursery conditions.

2. seedlings_height - height (cm) and root collar diameter (mm) after one year growth of seedlings received from seeds stored at conditions mentioned above.

All data comes from study realised in Institute of Dendrology Polish Academy of Science.

10.6084/m9.figshare.9080513.v1

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The comet assay as a method for assessing dna damage in cryopreserved samples.

PubMed (2024)

Beata P. Plitta‐Michalak Alice A. Ramos Dominika Stępień Magdalena Trusiak Marcin Michalak

The preservation of the nuclear genome's integrity is paramount for the viability and overall health of cells, tissues, and organisms. DNA, being susceptible to damage under physiological conditions and vulnerable to both endogenous and environmental factors, faces constant threats. To assess DNA damage and repair within individual eukaryotic cells, the comet assay presents itself as a versatile, gel electrophoresis-based, relatively simple, and highly sensitive method. Originally designed to monitor DNA damage and repair within populations of mammalian cells, the comet assay has now found applications across diverse domains, including yeast, protozoa, plants, and invertebrates. This technique has proven invaluable in cryopreservation studies, serving as a valuable adjunct for determining suitable cryopreservation protocols. These protocols encompass choices related to cryoprotectants, sample preparation, as well as storage conditions in terms of time and temperature. In the realm of animal cryopreservation research, the comet assay stands as a gold-standard method for assessing DNA integrity. Nevertheless, when applied in plant-oriented investigations, additional efforts are essential due to the distinct nature of plant cells and associated technical challenges. This review elucidates the fundamental principles underlying the comet assay, discusses its current iterations, and delineates its applications in the cryopreservation of both animal and plant specimens. Moreover, we delve into the primary challenges confronting the comet assay's utility as a monitoring tool in the context of plant sample cryopreservation. https://doi.org/10.54680/fr24110110112.

Comet Assay

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