Cervical cancer (CC) is the fourth leading cause of cancer‑associated mortalities among women worldwide. The chemotherapeutical platinum‑based agent cisplatin (DDP) is the standard therapy for locally advanced or recurrent CC; however, platinum resistance limits its clinical benefit. Therefore, the present study aimed to identify key genes associated with DDP resistance in patients with CC and investigate the underlying molecular mechanisms. Firstly, using the CRISPR‑Cas9 dataset of CC cells derived from DepMap portal, 699 genes associated with CC cell survival were identified. Subsequently, using the gene expression profiles of normal and CC samples with a response status to DDP, derived from The Cancer Genome Atlas (TCGA), hypoxia upregulated 1 (HYOU1) was further identified as significantly upregulated in CC samples and patients that did not respond to DDP (non‑responders) when compared with healthy controls and patients that did respond to DDP (responders), respectively, using unpaired student's t‑tests. Additionally, the log‑rank test revealed that the high expression of HYOU1 was significantly associated with the poor survival of patients receiving DDP. The association between the high HYOU1 expression levels and the poor survival of patients receiving DDP was validated in the remaining TCGA dataset of patients with CC. HYOU1 expression levels were positively associated with the half‑maximal inhibitory concentration value of DDP in CC cells using data derived from the Genomics of Drug Sensitivity in Cancer database. In vitro, western blotting experiments revealed high HYOU1 protein expression levels in DDP‑resistant HeLa cells compared with their parental HeLa cells. Furthermore, the knockdown of HYOU1 resulted in an increased sensitivity of HeLa cells to DDP. Finally, using the sequence‑based RNA adenosine methylation site predictor program, it was found that N6‑methyladenosine (m6A) was highly enriched in HYOU1. The expression levels of the m6A reader, EIF3A, was positively correlated with the expression levels of HYOU1 and was upregulated in the non‑response group compared with the response group in a dataset from TCGA database. Additionally, EIF3A had the highest probability of binding to the m6A motifs of HYOU1 compared with other genes. In GSE56363 obtained from the Gene Expression Omnibus, the non‑responders had significantly increased expression levels of EIF3A compared with the responders. In conclusion, high expression levels of HYOU1, which may be regulated by EIF3A due to m6A modifications, was associated with DDP resistance in patients with CC and could potentially be used as an indicator of DDP treatment resistance.
Space robots are an effective resource for astronauts working in a dangerous space environment. This paper proposes and implements a system to validate the performance of robot movement in space. A humanoid robot was designed with a vision-based self-calibration and navigation system. In addition, a path planning method was proposed to minimize joint torque. Simple gravity-compensation equipment with active and passive mechanisms was proposed. However, the flexible connection required for free movement between the robot and the gravity-compensation equipment meant that the space robot was likely to vibrate when moving. In order to address this challenge, a new hybrid force-position controller with joint torque feedforward was proposed. This controller was based on the system dynamics model with a particular focus on joint dynamics. Experimental test results validated the system design and methodology, showing that the humanoid space robot could move sufficiently using simple gravity-compensation equipment.
Abstract Background Begging brings benefits and costs for nestling birds: it can indicate their needs to their parents, but it can also be a cue used by predators to find the nest. The costs, like many variables related to nest predation, can be specific to what kinds of predators are present and their auditory capabilities. These costs and benefits could also be affected by human noise, as noise could disrupt communication to parents and eavesdropping by predators, although human-produced noise might be easily ignored if predators can hear high-frequency components of the begging. We studied nest predation on a generalist bird, the Red-whiskered Bulbul Pycnonotus jocosus , in a tropical rainforest in which there are many kinds of nest predators, including birds, mammals and reptiles. Results In a total of 160 artificial nests, we broadcast recordings of begging nestlings, with and without traffic noise, at two volume levels. We hypothesized that playback would increase predation relative to a silent control, and that mixing in traffic noise with the begging would decrease predation, as the begging signal was masked. However, we hypothesized that some predators, particularly small mammals with sensitive high-frequency hearing, might ignore the traffic noise. We found that predation was lowest for the control treatment, and lower for treatments mixed with traffic noise than for those without it. Small mammals, however, showed an unexpected pattern, strongly avoiding the treatments with traffic noise. Conclusion Our results demonstrate the human-associated noise can disturb nest predators and influence which kinds of predators use begging to locate nests.
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